Field-theoretical approach to particle oscillations in absorbing matter

The $ab$ oscillations in absorbing matter are considered. The standard model based on optical potential does not describe the total $ab$ transition probability as well as the channel corresponding to absorption of the $b$-particle. We calculate directly the off-diagonal matrix element in the framework of field-theoretical approach. Contrary to one-particle model, the final state absorption does not tend to suppress the channels mentioned above or, similarly, calculation with hermitian Hamiltonian leads to increase the corresponding values. The model reproduces all the results on the particle oscillations, however it is oriented to the description of the above-mentioned channels. Also we touch on the problem of infrared singularities. The approach under study is infrared-free.Comment: 27 pages, 8 figure

A Universal Nucleoside For Use At Ambiguous Sites In Dna Primers

A NON-DISCRIMINATORY base analogue, or universal base, would be an invaluable component of oligonucleotide probes and primers for solving the design problems that arise as a result of the degeneracy of the genetic code, or when only fragmentary peptide sequence data are available. We have designed an alternative to previous universal nucleoside candidates(1-9), a new analogue, 1-(2'-deoxy-beta-D-ribofuranosyl)-3-nitropyrrole (designated M; Fig. 1), which maximizes stacking while minimizing hydrogen-bonding interactions without sterically disrupting a DNA duplex. Oligonucleotides containing M at several sites were used as primers for sequencing and the polymerase chain reaction. The sequencing primer d(5'-CGT AAM CAM AAM ACM AT-3') is as effective as the exact match d(5'-CGT AAT CAG AAA ACA AT-3'). It is also possible to sequence using a primer containing M at several contiguous positions, for example d(5'-CGT AAT MMM MMM MMM AT-3'). Melting curves show that duplexes formed on hybridization of the sequences d(5';CCT TTT TMT TTT TGG-3') and d(5'-CCA AAA AXA AAA AGG-3'), where X is A, C, G or T, melted at a lower temperature than the corresponding duplexes containing only d(A.T) and d(C.G) base pairs, but showing little variation among different X bases (T-m range 3 degrees C).Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62640/1/369492a0.pd