TOXOPLASMOSIS: MODERN STRATEGIES OF LABORATORY DIAGNOSTICS

Abstract. Modern conceptions concerning congenital and acquired toxoplasmosis and some indications for patients’ examination are discussed in the paper. Methods of toxoplasmosis laboratory diagnostics focused on DNA, toxoplasms’ antigens, IgM, IgA, IgG antibodies identification with avidity determination and Line-blot evidence have been elucidated. Liquor analysis specificity in cerebral damage in HIV patients and acquired toxoplasmosis has been reproduced

IMMUNOLOGIC MARKER OF INTRANATAL INFECTIONS

The results of the research established determination of immunologic marker for prognosis of intranatal infection. The authors performed a comparative study of the immunity values of women in a risk group for prenatal infection. As it was shown when prenatal infection develops, there is an increase in the content of proinflammatory cytokines in the pregnant woman serum and activation of the ingestion rate. Account the results to devise an algorithm the pregnant woman care

ESTIMATION OF PREVALENCE OF ADVERSE GENOTYPES AND POLYMORPHIC ALLELES OF GENES-CANDIDATES OF ARTERIAL HYPERTENSION IN CHILDREN WITH ENDEMIC GOITER IN ZABAYKALSKY KRAI

This study is devoted to study the prevalence of genotypes and polymorphic alleles of genes-candidates of arterial hypertension in children with endemic goiter (EZ) in the Zabaykalsky Krai. To identify the frequency of occurrence of adverse genotypes and alleles polymorphisms Gly16Arg ADRB2, Gln27Glu ADRB2 and Thr174Met gene AGT, as factors of risk of development of arterial hypertension, were examined 194 children aged 7-11 years with a diagnosis of endemic goiter. The frequency of mutant allele 16Gly genotype Gly16Arg associated with increased agonist-induced suppression of beta-2 adrenergic receptors has not been above (63,2 %) than in the group of healthy children (60,3 %) (p(j2) > 0.05). The frequency of allele Arg16 defining a greater degree of vasodilating effect, was 39.7 % and 36.8 %, respectively (p(x2) > 0.05). Thefrequency characteristics of alleles genotype Gln27Glu in group I were the following: wild type 27Glu 41 %, the mutant allele Gln27 59 %, in the control group 42,2 % (p(χ2) > 0.05) and 57.8 % (pfy2) > 0.05) respectively. The frequency of wild-type Thr174 genotype Thr174Met accounted for 85.3 % in group I and 86.2 % in the control group (p(x2) > 0.05) and the frequency of occurrence ofthe mutated allele 174Met 14.7 % in the group of children with diffuse nontoxic goiter and 13.8 % in healthy children, respectively (pCx2) > 0.05). Significant differences in the distribution of genotypes and alleles between groups are not identified, and the frequency characteristics were comparable with the prevalence in the population and the results of other authors. The distribution of genotypes and frequency of alleles of a gene Gly16Arg and Gln27Glu ADRB2, Thr174Metgene AGT in both groups corresponded to the expected according to the hardy-Weinberg equilibrium in the population sample. The results obtained in the course of molecular-genetic study led to the conclusion that polymorphisms Gly16Arg ADRB2, Gln27Glu ADRB2 and Thr174Met gene AGT are not factors of risk of development of arterial hypertension in children with endemic goiter

Study of apolipoprotein C3 S1/S2 (C3238G, rs5128) polymorphism in Caucasian population of Eastern Siberia

The prevalence of genotypes and alleles of polymorphic marker S1 /S2 Apolipoprotein C3 in healthy adolescent Caucasians (Russians) living in Eastern Siberia was studied. Material for research was the DNA extracted from the venous blood; genotype was determined by PCR-RFLP for each sample. In the studied sample in 88.9 % of cases genotype S1/ S1 was detected, genotype S1/S2 was found in 8.6 %, S2/S2 - in 2.5 %. The share of S2 allele was 6.8 %, which is not significantly different from the previously studied Caucasoid populations

NUTRITCIOGENETICS AND RISK FACTORS OF CARDIOVASCULAR DISEASE: ASSOCIATED RESEARCH IN EASTERN SIBERIA POPULATIONS

Purpose: to study nutritional habits as a risk factor of cardiovascular pathology as so as a role of apolipoprotein A1 gene in metabolism in native and alien population of Eastern Siberia. Methods: we used Kiselev questionnaire (1998) for estimation of "nutritional risk". Totally 863 adolescents were evaluated, including 445 (51,6 %) persons of the alien ethnogroup (Russian) and 418 (48,4 %) persons of the native ethnogroup (Buriat). We evaluated clinically, functionally and genetically healthy and hypertensive (having essential arterial hypertension) adolescents and compared the results. Out of 226 adolescents with essential arterial hypertension, 144 persons were from the alien ethnogroup (mean age is 16,22 ± 1,14 years) and 82 adolescents from the native ethnogroup (mean age is 16,57 ± 1,62 years). Out of 173 healthy adolescents (mean age is 15,12 ± 2,71 years), 79 (45,7 %) persons were buriats and 94 (54,3 %) persons were Russian. Ethnic data were gotten by questionnaire, which included information about nationality back to the third generation of ancestors. We studied a total genomic DNA, which was extracted from blood serum by non-enzymatic method. An amplification of DNA loci was performed by polymerase-chain reaction using an automatic thermocycler «Biometra» with «SNP-экспресс», «Litex» reagents. The results of our research indicated an imbalance of nutritional habits with domination of protein-lipid dietary compounds in adolescents of native and alien population ofEastern Siberia. We found differentiated contributions ofinsertion-deletional polymorphism of apolipoprotein A1 gene. We did no found differences of frequency response of alleles and genotypes. We found a positive linkofapolipoprotein Al gene deletion with increased level ofcholesterine, very low density lipoproteins, triglycerides as so as with elevation ofnumbers ofblood pressure indexes in the adolescents ofthe alien ethnogroup. We did not found such kind of links in adolescents of the native ethnogroup that, as we suppose, may be a reflection of the evolutionary developed nutritional stereotype with some predominance oflipid-protein dietary component. Developed through the millennia such kind ofdiet in the indigenous population of the North and Siberia is a response to cold stress and underlies formation ofthe genotype, which aimed to developing a biochemical phenotype with ability to compensate this nutritional atherogenic imbalance

COMPARATIVE CHARACTERISTICS OF REGIONAL FEATURES OF AVERAGE VALUES OF SP AND DP IN AGE AND ETHNOSEXUAL ASPECT

4615 children have been screened: 2410 [52.2 %] are Russians, and 2205 [47.8 %] are Buryats; 2159 [46,8 %] of them are boys, and2454 [53,2 %] are girls. The widely spread risk factors of arterial hypertension development are heritable heart diseases, smoking (including passive smoking), overweight, as well as a sedentary way of life in different sex, age and ethnic groups. The results of the screening showed that the hypodynamia are more typical for city-dwellers, than for villagers. It is proved that high blood pressure prevalence is dependent on the phase of sexual development of girls belonging to both ethnic groups

Features of the Processes of Lipoperoxidation and Antioxidant Protection in the Pathogenesis of Infertility in Men of Different Ethnic Groups, Carriers of Nonfunctional Polymorphisms of the GSTT1 and GSTM1 Genes

A comparative analysis was carried out of the parameters of lipoperoxidation and antioxidant protection in fertile and infertile men of two ethnic groups (Caucasians and Mongoloids), who were carriers of different genotypes of xenobiotic biotransformation genes (GSTT1 and GSTM1). In serum and ejaculate, we determined the content of thiobarbituric acid (TBA-AP), α-tocopherol, reduced glutathione (GSH) and oxidized glutathione (GSSG); the activity of glutathione-S-transferase (GST), glutathione peroxidase (GPO) and glutathione reductase (GR); and total antioxidant activity (AOA). It was found that in Caucasian fertile men, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), there was a decrease in GST activity in serum and ejaculate with an increase in AOA in these substrates in comparison with similar indicators in carriers of genotypes GSTT1(1+/1+)/GSTM1(1+/1+). In Mongoloid fertile men, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), in comparison with similar indices in carriers of genotypes GSTT1(1+/1+)/GSTM1(1+/1+), we found an increase in serum content of α-tocopherol and activity of GPO, a decrease in GST activity, and a decrease in the level of α-tocopherol in the ejaculate. In Caucasians with infertility, carriers of genotypes GSTT1(*0/*0)/GSTM1(0*/*0), we found decreased activity of GST in the blood and ejaculate. In this group, we also registered a decrease in the GSH level, with an increase in the level TBA-AP and GPO activity. In infertile Mongoloids, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), we found an increase in the level TBA-AP and GPO activity and a decrease in serum α-tocopherol level