Investigations of chair of the general pharmaceutical and biomedical technology in the field of bioengineering of vegetative producers of biologically active substances

Article is devoted to the complex researches of various morphological groups of raw materials, a cellular biomass and a productive part of metaboloms of vegetative superproducers of a natural and biotechnological origin. All investigations were carried out according to the Federal special purpose program «Scientific and scientific and pedagogical shots of innovative Russia» for 2009-2013, action item № 1.2.1"Carrying out of scientific researches by scientific groups under the direction of doctors of sciences», Project: «Bioengineering as a basis of mobilization of adaptive potential of bioobjects - superproducers BAS» GK П555/05.08.2009 - НК-97П

Phenotypic, biochemical and genomic variability in generations of the rapeseed (Brassica napus L.) mutant lines obtained via chemical mutagenesis.

The phenotypic, biochemical and genetic variability was studied in M2-M5 generations of ethyl methansulfonat (EMS, 0.2%) mutagenized rapeseed lines generated from canola, '00', B. napus cv. Vikros. EMS mutagenesis induced extensive diversity in morphological and agronomic traits among mutant progeny resulted in selection of EMS populations of B. napus- and B. rapa-morphotypes. The seeds of the obtained mutant lines were high-protein, low in oil and stabilized in contents of main fatty acids which make them useful for feed production. Despite the increased level of various meiotic abnormalities revealed in EMS populations, comparative karyotype analysis and FISH-based visualization of 45S and 5S rDNA indicated a high level of karyotypic stability in M2-M5 plants, and therefore, the obtained mutant lines could be useful in further rapeseed improvement. The revealed structural chromosomal reorganizations in karyotypes of several plants of B. rapa-type indicate that rapeseed breeding by chemical mutagenesis can result in cytogenetic instability in the mutant progeny, and therefore, it should include the karyotype examination. Our findings demonstrate that EMS at low concentrations has great potential in rapeseed improvement