The role of antioxidants and their use in animal breeding and poultry farming (review)

The article reviews the role of antioxidants and their use as additives in feed for various types of farm animals and poultry. Incoming antioxidants activate protection. This protection is based on preventing the leakage of free electrons in mitochondria by cleaning intermediate radicals with vitamins, glutathiones, uric acid, bilirubin, ubiquinone, etc. Damaged molecules are restored and removed and the synthesis of new molecules is activated. Feed additives of natural origin are characterized by a high content of antioxidants. The use of these additives can improve the efficiency of animal and poultry farming. Facts about the presence of natural antioxidants in plants are outlined in the review. Phenolic compounds and flavonoids forming the basis of natural antioxidants play a vital role in preventing diseases associated with both oxidative stress, which produces free radicals, and as inhibitors of pathogenic microflora. Organoleptic changes affecting the period of storing occur during the oxidation of livestock products during storage. The presence of antioxidants limits the degree of oxidation of livestock products. Synthetic antioxidants are forbidden in many countries although they are effective in inhibiting oxidation in food. A natural alternative to traditional synthetic antioxidants for the purpose of using them as preservatives for livestock products is becoming more preferable, since until now the harm for the consumer from the action of natural antioxidants in comparison with synthetic ones has not been proved: natural antioxidants effectively slow down lipid oxidation

CHEMOKINE MARKERS ASSOCIATED WITH EARLY REJECTION OF KIDNEY ALLOGRAFT

It is known at the present time that immunological biomarkers may become more sensitive, non-invasive methods of graft rejection diagnosis than those currently used. A growing amount of studies in animal models shows that chemokines, as active participants in the immune process, may be used to this purpose. Our earlier studies have shown an important prognostic significance of IL-6, IL-2, 17A and IL-1RA increase in pre-operative period as markers of acute kidney allograft rejection. When assessing changes in studied peripheral blood growth factors, we concluded that a sharp decrease in BDNF content is a diagnostically significant early sign of kidney allograft rejection. The aim of this study was to identify the prognostic role of serum chemokine levels at the preoperative stage, taking into account the production of anti-HLA antibodies during the post-transplant period as a risk factor of kidney allograft rejection. A comparative analysis of chemokine serum concentrations was performed in the patients with terminal-stage chronic kidney disease (CKD). In the patients from main clinical groups, the blood cytokine levels were measured 6 hours before transplantation, i.e., Eotaxin (CCL11), GRO-α (CXCL1), IL-8 (CXCL8), IP-10 (CXCL10), MCP-1 (CCL2), MIP-1α (CCL3), MIP-1β (CCL4), SDF-1α (CXCL12), RANTES (CCL5), MIG (CXCL9) by means of multiplex immunological assays, using appropriate test systems. The studies have shown significant changes in several chemokines in the CKD patients compared to age-matched controls. However, the following diagnostically significant biomarkers associated with early rejection of transplanted kidney should be considered: increased concentration of CCL2 and CCL4 chemokines, as well as an acute decrease in CCL11. Significantly decreased CXCL12 concentration in peripheral blood could be considered a marker of favorable posttransplant clinical course.  Occurence of HLA antibodies in recipients is also associated with elevated serum levels of CXCL8, CXCL10, CCL4, and CCL5

Effect of antioxidants in a liposomal form containing organic iodine of the blood serum biochemical composition and the structure of muscle tissue formation of young rabbits

The study of the effect of antioxidants containing organic iodine on the formation of the enteral environment of California young rabbits is described in the article. A significant increase in the level of thyroxine in the experimental groups was in the 2nd by 6.7% and in the 3rd by 8.9% (P<0.05). In the third group, there was a significant tendency to increase the total protein in the blood serum: compared with the control, it increased by 3.39 units (P<0.05). This trend continues in the level of albumin at 3.58 units. (P<0.05). There was a significant increase in creatinine in young rabbits of both experimental groups by 20.51-28.89 units (P<0.05). There was a significant excess of iodine content in the meat of rabbits of group 2 by 81.0 mcg (P<0.05), and in animals of group 3-by 234.1 % (P<0.05). There was an increase in the number of muscle fibers in both experimental groups by 68.0 and 78.0% (P<0.05-0.01). The diameter of the muscle fiber of experimental animals was significantly lower by 13.1 and 17.3 % (P<0.05). The animals of the experimental groups formed meat with a more delicate structure and an increased content of valuable parts-muscle and fat tissue

Mathematical methods in the breeding evaluation of small horned ruminants

Aim. Traditionally, prediction of breeding values of male small horned ruminants   (rams) by referring to levels of economically useful traits of their progeny is carried  out by methods of statistical analysis. However, at the same time, there is a forecasting method based on the use of a mixed biometric model. The solution of the system  of equations constituting a mixed biometric model is associated with certain difficulties caused by the peculiarity of the system matrix. It is proposed to use integrated  mathematical packages in the forecast, by which the system of equations can be  solved in several ways, followed by analysis of the results. The prediction of progeny  values is carried out by statistical methods using three statistical tests, as well as with  the use of a mixed biometric model. It is of interest to compare estimates obtained  by using statistical methods with estimates using a mixed biometric model. Material and Methods. The initial data set was the live weight of Qigai rams, the  progeny of a group of sixteen rams belonging to eight genetic groups.   Results. It was found that the forecast of breeding values of each animal using a  mixed biometric model substantially clarifies the rank of each animal in the group  being evaluated.   Conclusion. The refinement of the estimation of breeding value is related to the  effects of the genetic groups to which the animals belong in the mixed model, as well  as the degree of relationship between them. Also the mixed model also allows one to  isolate environmental effects from the overall assessment. Solving the system of  equations in several ways will improve the reliability of the forecast

Estimation of the current of sharp purulent inflammatory processes by means of algorithms of the computer analysis of images

The study describes the automatic algorithm for image analysis based on software Cellprofiler. For the main example of the principal implementation of the analysis process agar culture swabs of Staphylococcus aureus were taken. The proposed algorithms of recognition of microbial elements were verified and showed a significantly higher efficiency compared to the visual method of counting by the laboratory specialist