38 research outputs found
Magnesia Binder Preparation from Local Natural and Technogenic Raw Materials
The preparation of magnesia binders based on natural and technogenic mineral was the result of the research. The obtained magnesia binders possess the increased flexural and compressive strength which values are close to ones of natural materials. High flexural and compression strengths are associated with the features of the hardened caustic dolomite containing magnesium oxyhydrochlorides which crystallize as a fiber. The fibrous crystals not only increase the cement strength, but also act as a reinforcing material. The resulting magnesia binder does not require a moist environment at hardening. It is characterized by decorativeness and ecological compatibility, has a neutral hardening product composition
Magnesia Binder Preparation from Local Natural and Technogenic Raw Materials
The preparation of magnesia binders based on natural and technogenic mineral was the result of the research. The obtained magnesia binders possess the increased flexural and compressive strength which values are close to ones of natural materials. High flexural and compression strengths are associated with the features of the hardened caustic dolomite containing magnesium oxyhydrochlorides which crystallize as a fiber. The fibrous crystals not only increase the cement strength, but also act as a reinforcing material. The resulting magnesia binder does not require a moist environment at hardening. It is characterized by decorativeness and ecological compatibility, has a neutral hardening product composition
THE EXPERIENCE OF IMPLEMENTING P. YA. GALPERINβS APPROACH IN THE PROCESS OF FORMING STUDENTSβ COMMUNICATIVE COMPETENCE
The article substantiates the importance of the formation of communicative competence at the present stage of education development, examines the role of group forms of learning in the formation of communicative skills. The authors present the experience of applying the concept of step-by-step formation of mental actions of P. Ya. Galperin for the development of studentsβ communicative competence.Π ΡΡΠ°ΡΡΠ΅ ΠΎΠ±ΠΎΡΠ½ΠΎΠ²ΡΠ²Π°Π΅ΡΡΡ Π²Π°ΠΆΠ½ΠΎΡΡΡ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΠΎΡΡΠΈ Π½Π° ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΌ ΡΡΠ°ΠΏΠ΅ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ, ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°Π΅ΡΡΡ ΡΠΎΠ»Ρ Π³ΡΡΠΏΠΏΠΎΠ²ΡΡ
ΡΠΎΡΠΌ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠ²Π½ΡΡ
ΡΠΌΠ΅Π½ΠΈΠΉ. ΠΠ²ΡΠΎΡΠ°ΠΌΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ ΠΎΠΏΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅ΠΏΡΠΈΠΈ ΠΏΠΎΡΡΠ°ΠΏΠ½ΠΎΠ³ΠΎ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΌΡΡΠ²Π΅Π½Π½ΡΡ
Π΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π.Π―. ΠΠ°Π»ΡΠΏΠ΅ΡΠΈΠ½Π° Π΄Π»Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΠΎΡΡΠΈ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ²
Effect of Additive of Polymetallic Oresβ Tailings on Properties of Composite Cements
The article analyzes the use of polymetallic oresβ tailings as a basis for production of composite cements and concrete, having protective properties against gamma and X-rays radiation, as well as high strength and lifetime. The main practically significant result is: the development of scientific and technological production bases of new high-tech type of multicomponent hydraulic binders for concretes β composite cements; the identification of new hydration products in composite cements with addition of polymetallic oresβ tailings; the development of optimal compositions of composite cements for concretes. It is established that the composite cements, that developed by us on the basis of polymetallic oresβ tailings, meet modern requirements i.e. its improve the construction-technical properties of material, have positive effect to the environment situation and allow to reduce the production cost of the final product. Their technology is low metal-intensive and power-consuming. Studies of physical-chemical processes of composite cements structure formation with addition of polymetallic oresβ tailings have been conducted using methods such as chemical, X-ray phase, differential-thermal and electronmicroscopic analysis methods
Development and Testing of Low-Energy-Intensive Technology of Receiving Sulphate-Resistant and Road Portlandcement
The article presents the results of obtaining clinkers of sulfate resistant and road cement in the process of production tests. The use of man-made waste (lead slag, coal mining waste) as a clay component and additive is analyzed. X-ray, chemical analyzes of starting materials, clinkers and cements were carried out. The regularities of the influence of the compositions of charge, SC, modules, burning regimes on the chemical and mineralogical composition and quality of clinkers and cements, improvement of the rotary kiln operation, reduction of greenhouse gas emissions and fuel consumption are established. Various methods of analysis were used, calculations of the composition of raw mixtures were carried out according to the program "Shikhta" and "RCC". The strength of factory and experimental cements was tested after 3, 7 and 28 days and after steaming. The physico-mechanical characteristics and structure of cements have been studied. We have developed and tested low energy-intensive technology and compiled a technological procedure for obtaining sulfate-resistant and road cements
Development of low-temperature thermochemical conversion reactors for coal power engineering
The main principles applied in developing a technology for low-temperature thermochemical conversion of brown coals to obtain fuel gas and semicoke intended for being fired in two-fuel power installations are considered on the basis of a set of experimental and calculated investigations. The obtained results are compared with the experimental data obtained using other methods and with the results of previous industrial tests. Β© 2013 Pleiades Publishing, Inc
Improving the Ranking of Russian Smart Cities
ΠΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΠΉ ΡΠΈΡΡΠΎΠ²ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ ΡΡΠ±Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π±ΠΎΠ»ΡΡΡΡ ΡΠΎΠ»Ρ Π² ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΠ°ΡΠ°Π΄ΠΈΠ³ΠΌΡ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΠ³ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΡΠ°Π» ΠΈΠ³ΡΠ°ΡΡ ΡΠΌΠ½ΡΠΉ Π³ΠΎΡΠΎΠ΄, Π²ΡΡΡΡΠΏΠ°Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΠΌ ΠΎΠ±ΡΠ΅ΠΊΡΠΎΠΌ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ ΡΡΡΠΎΠΉΡΠΈΠ²ΡΠΌ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ΠΌ. Π£ΠΌΠ½ΡΠΉ Π³ΠΎΡΠΎΠ΄ Π΄ΠΎΠ»ΠΆΠ΅Π½ ΡΡΠ°ΡΡ ΡΡΡΠΎΠΉΡΠΈΠ²ΡΠΌ ΡΠΌΠ½ΡΠΌ Π³ΠΎΡΠΎΠ΄ΠΎΠΌ, ΠΈ ΠΏΠ΅ΡΠ²ΡΠΉ ΡΠ°Π³ Π² ΡΡΠΎΠΌ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠΈ - ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° ΡΠΈΡΡΠ΅ΠΌΡ Β«Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΡΠ΅ΠΊΡΡΠ΅Π³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡΒ», ΠΈΠ»ΠΈ ΡΠ΅ΠΉΡΠΈΠ½Π³Π° ΡΠΌΠ½ΡΡ
Π³ΠΎΡΠΎΠ΄ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠΉ ΡΡΠΈΡΡΠ²Π°Π΅Ρ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΡΠ΅ ΡΡΠ°Π½Π΄Π°ΡΡΡ, ΡΠΏΠ΅ΡΠΈΡΠΈΠΊΡ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΎΡΡΠΈΠΉΡΠΊΠΈΡ
Π³ΠΎΡΠΎΠ΄ΠΎΠ², Π½ΠΎ ΠΈ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ. ΠΠΈΠΏΠΎΡΠ΅Π·Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΡΡΠΎΠΈΡ Π² ΡΠΎΠΌ, ΡΡΠΎ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΡΠ΅ΠΉΡΠΈΠ½Π³ΠΎΠ²Π°Π½ΠΈΡ Π΄ΠΎΠ»ΠΆΠ½Π° ΠΎΡΡΠ°ΠΆΠ°ΡΡ ΡΡΠΈ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ ΠΈ Π±ΡΡΡ ΠΎΡΠ½ΠΎΠ²ΠΎΠΉ Π΄Π»Ρ Π°Π½Π°Π»ΠΈΠ·Π° Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΠ³ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π² ΡΠ°Π·ΡΠ΅Π·Π΅ Π²ΡΠ±ΡΠ°Π½Π½ΡΡ
ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π² ΠΈ ΡΠ°ΠΊΡΠΎΡΠΎΠ². Π ΠΎΡΠ½ΠΎΠ²Π΅ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠ΅ΠΉ ΠΎΡΠ΅Π½ΠΈΠ²Π°ΡΡ Π³ΠΎΡΠΎΠ΄Π° Ρ ΡΠΈΡΠ»Π΅Π½Π½ΠΎΡΡΡΡ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΠ²ΡΡΠ΅ 100 ΡΡΡ. ΡΠ΅Π». Π² ΡΠ°Π·ΡΠ΅Π·Π΅ ΡΠΎΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ, ΡΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ, ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ ΡΠΏΡΠ°Π²Π»Π΅Π½ΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ Π»Π΅ΠΆΠ°Ρ ΡΠ΅ΠΎΡΠΈΡ ΠΌΠ½ΠΎΠ³ΠΎΠΌΠ΅ΡΠ½ΡΡ
ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΉ ΠΈ ΠΌΠ΅ΡΠΎΠ΄ ΡΠ°ΡΡΡΠΎΡΠ½ΠΈΠΉ. ΠΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½Π°Ρ ΠΎΡΠ΅Π½ΠΊΠ° ΡΠΌΠ½ΠΎΠ³ΠΎ Π³ΠΎΡΠΎΠ΄Π° Π²ΠΊΠ»ΡΡΠ°Π΅Ρ 71 ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ, ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΡΠ³ΡΡΠΏΠΏΠΈΡΠΎΠ²Π°Π½Ρ Π² 8 ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π²: ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ, ΡΠΎΡΠΈΠ°Π»ΡΠ½Π°Ρ ΡΠΏΠ»ΠΎΡΠ΅Π½Π½ΠΎΡΡΡ, ΡΠΊΠΎΠ½ΠΎΠΌΠΈΠΊΠ°, ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠ΅, ΡΠΊΠΎΠ»ΠΎΠ³ΠΈΡ ΠΈ ΠΎΠΊΡΡΠΆΠ°ΡΡΠ°Ρ ΡΡΠ΅Π΄Π°, ΡΡΠ°Π½ΡΠΏΠΎΡΡ, Π³ΡΠ°Π΄ΠΎΡΡΡΠΎΠΈΡΠ΅Π»ΡΡΡΠ²ΠΎ, ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΡΠ΅ΠΉΡΠΈΠ½Π³, Π²ΠΊΠ»ΡΡΠ°ΡΡΠΈΠΉ 171 Π³ΠΎΡΠΎΠ΄, ΠΈ Π²ΡΡΠ²Π»Π΅Π½Ρ ΡΠ΅Π³ΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΠΎΡΡΠΈΠΉΡΠΊΠΈΡ
ΡΠΌΠ½ΡΡ
Π³ΠΎΡΠΎΠ΄ΠΎΠ² Π² ΡΠ°Π·ΡΠ΅Π·Π΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΡΡ
ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π². Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π±Π°Π·ΠΎΠΉ Π²ΡΡΡΡΠΏΠΈΠ»ΠΈ ΡΠΎΡΡΠΈΠΉΡΠΊΠΈΠ΅ ΠΈ Π·Π°ΡΡΠ±Π΅ΠΆΠ½ΡΠ΅ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π±Π°Π·Ρ Π΄Π°Π½Π½ΡΡ
, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΎΡΡΠ°ΡΠ»Π΅Π²ΡΡ
Π°Π³Π΅Π½ΡΡΡΠ². Π ΠΏΡΡΠ΅ΡΠΊΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠ³ΠΎ ΡΠ΅ΠΉΡΠΈΠ½Π³Π° Π²ΠΎΡΠ»ΠΈ Π³ΠΎΡΠΎΠ΄Π° ΠΠΎΡΠΊΠ²Π°, Π‘Π°Π½ΠΊΡ-ΠΠ΅ΡΠ΅ΡΠ±ΡΡΠ³, ΠΠ°Π»Π°ΡΠΈΡ
Π°, ΠΡΠ°ΡΠ½ΠΎΠ΄Π°Ρ ΠΈ ΠΠ°Π·Π°Π½Ρ. Π‘ΡΠ΅Π΄ΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ
Π²ΡΠ²ΡΠ»Π΅Π½Π½ΡΡ
ΡΠ΅Π³ΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ
ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ - Π»ΠΈΠ΄Π΅ΡΡΡΠ²ΠΎ Π¦Π΅Π½ΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π΄Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠΊΡΡΠ³Π°, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠΈΠ»ΡΠ½Π°Ρ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°ΡΠΈΡ ΠΏΠΎ Π³ΡΡΠΏΠΏΠ°ΠΌ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Β«Π³ΡΠ°Π΄ΠΎΡΡΡΠΎΠΈΡΠ΅Π»ΡΡΡΠ²ΠΎΒ» ΠΈ Β«ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈΒ». ΠΠΆΠ΅Π³ΠΎΠ΄Π½ΡΠΉ ΡΠ°ΡΡΠ΅Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΉ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Π²ΡΡΠ²ΠΈΡΡ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΡ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΌΠ½ΡΡ
Π³ΠΎΡΠΎΠ΄ΠΎΠ², ΠΎΡΠ΅Π½ΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π΅ΠΌΡΡ
ΡΠ΅ΡΠ΅Π½ΠΈΠΉ ΠΈ Ρ
ΠΎΠ΄ ΠΈΡ
ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ, ΠΏΠ»Π°Π½ΠΈΡΠΎΠ²Π°ΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ Π³ΠΎΡΠΎΠ΄ΡΠΊΠΎΠ³ΠΎ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π° Π² ΡΠ°ΠΌΠΊΠ°Ρ
ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ΅Π΄Π΅ΡΠ°Π»ΡΠ½ΡΡ
ΠΈ ΡΠ΅Π³ΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ
ΠΏΡΠΎΠ΅ΠΊΡΠΎΠ² ΡΠΈΡΡΠΎΠ²ΠΈΠ·Π°ΡΠΈΠΈ Π Π€, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΠΈ Π³ΠΎΡΠΎΠ΄ΡΠΊΠΈΡ
Π°Π³Π»ΠΎΠΌΠ΅ΡΠ°ΡΠΈΠΉ ΠΏΡΠΈ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΡΠΎΠ΅ΠΊΡΠ° Β«Π£ΠΌΠ½ΡΠΉ Π³ΠΎΡΠΎΠ΄Β».Digitalisation and urbanisation have led to smart city becoming a key element of the concept of sustainable development, acting as an object of sustainable development management. Thus, a smart city must become a sustainable smart city. The first step is the creation of the system of current state diagnostics or smart city ranking. This ranking should consider international standards, the specificity of Russian urban development, as well as various components of sustainable development. It is hypothesised that the ranking methodology reflecting these components can be used to analyse the dynamics of sustainable development based on the chosen criteria and factors. To assess social, environmental, economic and management components of cities with a population of more than 100,000 people, the proposed technique relies on the theory of multiple comparisons and the distance method. The comprehensive assessment of smart cities includes 71 indicators grouped into 8 criteria: people, social cohesion, economy, management, ecology and the environment, transport, urban planning, technology. The study analysed Russian and international statistical databases, as well as data from industry agencies. The ranking of 171 cities was developed; regional features of Russian smart cities were identified using the selected criteria. The cities of Moscow, St. Petersburg, Balashikha, Krasnodar and Kazan are at the top of the ranking. The leadership of the Central Federal District and a significant differentiation by the criteria βurban planningβ and βtechnologyβ are the key regional features. Annual comprehensive assessment can be used to track the dynamics of the development of smart cities, evaluate the effectiveness of decision-making and implementation, plan urban development based on federal and regional digitalisation projects, as well as create strategies to enhance the sustainability of Russian smart cities
Synthetic gene for the hepatitis C virus nucleocapsid protein.
A synthetic gene encoding the hepatitis C virus (HCV) nucleocapsid protein was constructed and expressed in E. coli. To synthesize this gene, we developed a new method that results in the enzymatic synthesis of long polydeoxyribonucleotides from oligodeoxyribonucleotides. The method, designated as the 'Exchangeable Template Reaction' (ETR), uses oligonucleotides as templates for DNA polymerase. A special mechanism was designed to exchange the templates during the polymerase reaction. The mechanism relies on the formation of a single-stranded 3'-protrusion at the 'growing point' of the elongating DNA such that it can be subsequently annealed, in a sequence-specific manner, with the next synthetic oligonucleotide. When annealed to the 3'-protrusion, the added oligonucleotide becomes a template for DNA polymerase, and the protruding 3'-end of the double-stranded DNA is used as the primer. The HCV nucleocapsid gene was assembled with DNA ligase from three fragments synthesized by ETR. The data verify that this method is efficient. The main advantage of ETR is the ability to combine more than two oligonucleotides in one tube together with polymerase and an enzymatic activity that produces a 3'-protrusion (e.g., BstXI) rather than the sequential addition of each component. The data demonstrate that as many as five oligonucleotides can be used simultaneously, resulting in a synthesized DNA fragment of designed sequence. The synthetic gene expressed in E. coli produced a 27 kDa protein that specifically interacted with antibodies from sera obtained from HCV-infected individuals
Πynamics of light-absorption variations induced in a bismuth silicate crystal by visible laser illumination
We present the results of experimental studies of the dynamics of the photoinduced optical absorption
in a bismuth silicate crystal subject to continuous laser irradiation with wavelengths of
532 and 655 nm. The semiconductor-laser light beam with the wavelength Ξ» = 655 nm causes
the crystal bleaching at this wavelength, whereas its exposure to a shorter-wavelength irradiation
from the optical-spectrum green region with a wavelength of 532 nm increases the optical
absorption at both wavelengths, of 532 and 655 nm. The experimental results are interpreted
using the theoretical model which assumes that the crystal has deep defect centers of two types
so that an electron at each of these centers can be in one of the states characterized by different
photoionization cross sections