15 research outputs found
Methods for microbial DNA extraction from soil for PCR amplification
Get PDFAmplification of DNA from soil is often inhibited by co-purified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types (1). DNA is also suitable for PCR amplification using various DNA targets. DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation. This method was compared to other DNA extraction methods with regard to DNA purity and size
Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen
Full text linkEffects of long-term benzene pollution on bacterial diversity and community structure in groundwater.
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Introduction of anaerobic dechlorinating bacteria into soil slurry microcosms and nested-PCR monitoring
No full textComparative assessment of methods for metagenomic DNA isolation from soils of different crop growing fields
No full textComparative analysis of environmental DNA extraction and purification methods from different humic acid-rich soils
No full textSPION-mediated soil DNA extraction and comparative analysis with conventional and commercial kit-based protocol
No full textDevelopment of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis
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