Stream-dependent development of higher visual cortical areas

Multiple cortical areas contribute to visual processing in mice. However, the functional organization and development of higher visual areas are unclear. Here, we used intrinsic signal optical imaging and 2-photon calcium imaging to map visual responses in adult and developing mice. We found that visually driven activity was well-correlated among higher visual areas within two distinct subnetworks resembling the dorsal and ventral visual streams. Visual response magnitude in dorsal stream areas slowly increased over the first two weeks of visual experience. By contrast, ventral stream areas exhibited strong responses shortly after eye opening. Neurons in a dorsal stream area showed little change in their tuning sharpness to oriented gratings while those in a ventral stream area increased stimulus selectivity and expanded their receptive fields significantly. Together, these findings provide a functional basis for grouping subnetworks of mouse visual areas and revealed stream differences in the development of receptive field properties

Analysis of linezolid and tigecycline as candidates for local prophylaxis via antibiotic-loaded bone cement

Objectives To assess the Gram-positive-specific antibiotic linezolid and the broad-spectrum antibiotic tigecycline for use in local antibiotic delivery via antibiotic-loaded bone cement. Methods Linezolid and tigecycline were added to Biomet bone cement at varying concentrations. Antibiotic elution over 1 week was quantified by HPLC-MS. The effect of wear on elution over 51 h was determined using a modified TE-66 wear tester. Eluted antibiotics were used to determine the MICs for a panel of clinically relevant bacteria. The impact strength of antibiotic-loaded samples was determined using a Charpy-type impact testing apparatus. Cytotoxicity of eluted antibiotics against MG-63 cells was evaluated using an MTT assay. Results Linezolid and tigecycline eluted from bone cement to clinically relevant levels within 1 h and retained activity over 1 week. Mechanical wear significantly reduced elution of tigecycline, but had little effect on elution of linezolid. Linezolid showed low cytotoxicity towards MG-63 cells with ≤300 mg/mL resulting in >50% cell activity. Cytotoxicity of tigecycline was higher, with an IC50 of 5–10 mg/L. Conclusions Linezolid and tigecycline retain activity after elution from bone cement. The concentration of tigecycline may need to be carefully controlled due to cytotoxicity. The effect of wear on bone cement may need to be considered if tigecycline is to be used for local delivery. Up to 10% linezolid can be added without affecting the impact strength of the bone cement. These results are promising indications for future investigation of these antibiotics for use in local antibiotic delivery strategies

The Structure of the Homunculus. III. Forming a Disk and Bipolar Lobes in a Rotating Surface Explosion

We present a semi-analytic model for shaping the nebula around eta Carinae that accounts for the simultaneous production of bipolar lobes and an equatorial disk through a rotating surface explosion. Material is launched normal to the surface of an oblate rotating star with an initial kick velocity that scales approximately with the local escape speed. Thereafter, ejecta follow ballistic orbital trajectories, feeling only a central force corresponding to a radiatively reduced gravity. Our model is conceptually similar to the wind-compressed disk model of Bjorkman & Cassinelli, but we modify it to an explosion instead of a steady line-driven wind, we include a rotationally-distorted star, and we treat the dynamics somewhat differently. Continuum-driving avoids the disk inhibition that normally operates in line-driven winds. Our model provides a simple method by which rotating hot stars can simultaneously produce intrinsically bipolar and equatorial mass ejections, without an aspherical environment or magnetic fields. Although motivated by eta Carinae, the model may have generic application to other LBVs, B[e] stars, or SN1987A's nebula. When near-Eddington radiative driving is less influential, our model generalizes to produce bipolar morphologies without disks, as seen in many PNe.Comment: ApJ accepted, 9 page

The magnetic Bp star 36 Lyncis, I. Magnetic and photospheric properties

This paper reports the photospheric, magnetic and circumstellar gas characteristics of the magnetic B8p star 36 Lyncis (HD 79158). Using archival data and new polarised and unpolarised high-resolution spectra, we redetermine the basic physical properties, the rotational period and the geometry of the magnetic field, and the photospheric abundances of various elements.}{Based on magnetic and spectroscopic measurements, we infer an improved rotational period of $3.83475\pm 0.00002$ d. We determine a current epoch of the longitudinal magnetic field positive extremum (HJD 2452246.033), and provide constraints on the geometry of the dipole magnetic field (i\geq 56\degr, $3210 {\rm G}\leq B_{\rm d}\leq 3930$ G, $\beta$ unconstrained). We redetermine the effective temperature and surface gravity using the optical and UV energy distributions, optical photometry and Balmer line profiles ($T_{\rm eff}=13300\pm 300$ K, $\log g=3.7-4.2$), and based on the Hipparcos parallax we redetermine the luminosity, mass, radius and true rotational speed ($L=2.54\pm 0.16 L_\odot, M=4.0\pm 0.2 M_\odot, R=3.4\pm 0.7 R_\odot, v_{\rm eq}=45-61.5$ \kms). We measure photospheric abundances for 21 elements using optical and UV spectra, and constrain the presence of vertical stratification of these elements. We perform preliminary Doppler Imaging of the surface distribution of Fe, finding that Fe is distributed in a patchy belt near the rotational equator. Most remarkably, we confirm strong variations of the H$\alpha$ line core which we interpret as due to occultations of the star by magnetically-confined circumstellar gas.Comment: Accepted by Astronomy and Astrophysic

Alphabetic Letter Identification: Effects of perceivability, similarity, and bias

The legibility of the letters in the Latin alphabet has been measured numerous times since the beginning of\ud experimental psychology. To identify the theoretical mechanisms attributed to letter identification, we report\ud a comprehensive review of literature, spanning more than a century. This review revealed that identification\ud accuracy has frequently been attributed to a subset of three common sources: perceivability, bias, and simi-\ud larity. However, simultaneous estimates of these values have rarely (if ever) been performed. We present the\ud results of two new experiments which allow for the simultaneous estimation of these factors, and examine\ud how the shape of a visual mask impacts each of them, as inferred through a new statistical model. Results showed that the shape and identity of the mask impacted the inferred perceivability, bias, and similarity space of a letter set, but that there were aspects of similarity that were robust to the choice of mask. The results illustrate how the psychological concepts of perceivability, bias, and similarity can be estimated simultaneously, and how each make powerful contributions to visual letter identification

General and craniofacial development are complex adaptive processes influenced by diversity

Complex systems are present in such diverse areas as social systems, economies, ecosystems and Biology and, therefore, are highly relevant to dental research, education and practice. AComplex Adaptive System in biological development is a dynamicprocess in which, from interacting Components at a lowerlevel, higher level phenomena and structures emerge. Diversity makes Substantial contributions to the performance of Complex Adaptive Systems. It enhances the Robustness of the process, allowing multiple responses to external stimuli as well as internal changes. From Diversity comes variation in outcome and the possibility of major change; outliers in the distributionenhance the tipping points. The development of the dentition is a valuable, accessible model with extensive and reliable databases for investigating the role of Complex Adaptive Systems in craniofacial and general development. The general characteristics of such systems are seen during tooth development: self-organisation; bottom-up emergence; multitasking; self-adaptation; variation; tipping points; Critical phases; and robustness. Dental findings are compatible with the Random Network Model, the Threshold Model and also with the Scale Free Network Model which has a Power Law distribution. In addition, dental development shows the characteristics of Modularity and Clustering to form Hierarchical Networks. The interactions between the genes (nodes) demonstrate Small World phenomena, Subgraph Motifs and Gene Regulatory Networks. Genetic mechanisms are involved in the creation and evolution of variation during development. The genetic factors interact with epigenetic and environmental factors at the molecular level and form complex networks within the cells. From these interactions emerge the higher level tissues, tooth germs and mineralised teeth. Approaching development in this way allows investigation of why there can be variations in phenotypes from identical genotypes; the phenotype is the outcome of perturbations in the cellular systems and networks, as well as of the genotype. Understandingand applying complexity theorywill bring aboutsubstantial advances not only in dental research and education but also in the organisation and delivery of oral health care

The antimicrobial activity and biocompatibility of a controlled gentamicin-releasing single-layer sol-gel coating on hydroxyapatite-coated titanium

Aims The aim of this study was to develop a single-layer hybrid organic-inorganic sol-gel coating that is capable of a controlled antibiotic release for cementless hydroxyapatite (HA)-coated titanium orthopaedic prostheses. Methods Coatings containing gentamicin at a concentration of 1.25% weight/volume (wt/vol), similar to that found in commercially available antibiotic-loaded bone cement, were prepared and tested in the laboratory for: kinetics of antibiotic release; activity against planktonic and biofilm bacterial cultures; biocompatibility with cultured mammalian cells; and physical bonding to the material (n = 3 in all tests). The sol-gel coatings and controls were then tested in vivo in a small animal healing model (four materials tested; n = 6 per material), and applied to the surface of commercially pure HA-coated titanium rods. Results The coating released gentamicin at > 10 × minimum inhibitory concentration (MIC) for sensitive staphylococcal strains within one hour thereby potentially giving effective prophylaxis for arthroplasty surgery, and showed > 99% elution of the antibiotic within the coating after 48 hours. There was total eradication of both planktonic bacteria and established bacterial biofilms of a panel of clinically relevant staphylococci. Mesenchymal stem cells adhered to the coated surfaces and differentiated towards osteoblasts, depositing calcium and expressing the bone marker protein, osteopontin. In the in vivo small animal bone healing model, the antibiotic sol-gel coated titanium (Ti)/HA rod led to osseointegration equivalent to that of the conventional HA-coated surface. Conclusion In this study we report a new sol-gel technology that can release gentamicin from a bioceramic-coated cementless arthroplasty material. In vitro, local gentamicin levels are in excess of what can be achieved by antibiotic-loaded bone cement. In vivo, bone healing in an animal model is not impaired. This, thus, represents a biomaterial modification that may have the potential to protect at-risk patients from implant-related deep infection