Sulphur fertilization on biochemical constituents of cabbage (Brassica oleracea var. capitata. L) in non-calcareous soil of Coimbatore district, Tamil Nadu

Sulphur is the fourth most important plant nutrient after nitrogen, phosphorus, and potassium, and it is becoming increasingly crucial in high-quality crop production (Bhoyar., 2019). Since limited work has been carried out regarding different sulphur sources on cabbage production in the Coimbatore district, the present study was undertaken to investigate the sulphur sources and levels on various biochemical constituents of cabbage. Hence a  field experiment was conducted in the farmer’s field at Viraliyur village, Thondamuthur block of Coimbatore district, Tamil Nadu to assess the effect of sulphur fertilization on improving the biochemical constituents of cabbage hybrid Saint. There were four different S sources (Elemental sulphur, Potassium sulphate, Gypsum, Single super phosphate) applied at five levels (0, 20, 40, 60 and 80 kg ha-1) and replicated thrice in a factorial randomized block design. The crop was fertilized with a  Soil Test Crop Response-prescribed dose of NPK (200:125:25 kg ha-1). The crop was harvested on 90th day and cabbage heads were analysed for various biochemical constituents like ascorbic acid, total soluble solids (TSS), titratable acidity (TA), chlorophyll content, glucosinolates (GLs), total phenol content (TPC), sulphur containing amino acid (methionine) and antioxidant enzyme activity (peroxidase). The influence of S fertilizers on biochemical constituents increased significantly with increasing levels of sulphur fertilization up to 80 kg S ha-1 excluding ascorbic acid content. The pooled data showed that gypsum applied at 80 kg ha-1 registered the maximum GLs (69.0 µmol g-1), TPC (31.9 mM 100g-1), methionine (32.3%), peroxidase activity (0.70 units min-1mg-1), TSS (7.64 0Brix), TA (0.64%), ascorbic acid (61.4 mg 100g-1) and total chlorophyll (1.21 mg g-1) in cabbage head. The lowest content of biochemical constituents viz., GLs (34.1 µmol g-1), TPC (8.10 mM 100g-1), methionine (17.6%) and peroxidase (0.31 units min-1mg-1) were observed in control applied NPK alone. There were positive and significant changes in the biochemical constituents of cabbage due to S application which confirms the improvement in the quality of cabbage head. The study concluded that gypsum was  the better sulphur source for improving the quality of cabbage

Ethyl 2-phenyl-3-(4-phenyl-1,2,3-selenadiazol-5-yl)-3-p-tolyl­propano­ate

In the title compound, C26H24N2O2Se, the selenadiazole ring is essentially planar [maximum deviation = 0.004 (3) Å]. The dihedral angle between the selenadiazole ring and the attached benzene ring is 50.17 (1)°. The crystal packing is stabilized by inter­molecular C—H⋯N inter­actions

シリアサンジャガイモYウイルス ニ タイスル ポリクローナルコウタイ ノ ショサクセ

ジャガイモYウイルス(PVY)はシリアのジャガイモ生産における主要ウイルスの一つとされ,ジャガイモ生産の阻害要因となっている。PVY感染の無い種イモの生産と供給が主要な防除手段であるが,そのためには,効果的なウイルス検査法が必要である。酵素結合抗体法(ELISA)は複数種類のジャガイモウイルス検出には最も一般的な技術であるが,それぞれのウイルスに対して特異的な抗血清が利用できることが必須条件となる。本研究では,シリアに発生したPVY分離株を純化し,ウサギに免疫することにより抗血清をはじめて作製した。この抗血清を利用し多くのシリア産PVYを用いたELISAによる検討では,市販の抗血清と同等の検出能力を有し,両抗血清間に結果の相違は認められなかった。また,非特異的反応も生じなかった。さらに,検出限界は高かった。以上より,本研究ではシリアで分離されたPVYに対して初めてシリア国産抗血清を得ることができ,抗血清の恒常的輸入を不要にしたことに大きな意義があると考えられる。Potato virus Y (PVY) is reported to be the main virus occurring in potato fields and it increases the cost of potato production in Syria. Producing and utilizing of certified seed potatoes with minimum PVY infection is the main control key that requires efficient virus detection methods. Enzyme linked immunosorbent assay (ELISA) using specific antisera is the most common method for the detection of potato viruses. In this study we report the production of PVY antiserum in Syria for the first time. This antibody was produced against a Syrian PVY isolate and its reliability was confirmed by testing a panel of PVY infected samples collected from Syria. The results of ELISA using this antibody were consistent with those obtained using Agdia PVY antibody and no significant differences were found. In addition this antibody was highly specific to PVY with undetectable cross reaction to plant proteins. Moreover it was shown to be very sensitive and PVY infection could be detected in highly diluted plant saps. Since this antibody was produced against a Syrian isolate of PVY it has advantages over imported PVY specific antiserum

Resonance enhanced isotope-selective photoionization of YbI for ion trap loading

Neutral Ytterbium (YbI) and singly ionized Ytterbium (YbII) is widely used in experiments in quantum optics, metrology and quantum information science. We report on the investigation of isotope selective two-photoionisation of YbI that allows for efficient loading of ion traps with YbII. Results are presented on two-colour (399 nm and 369 nm) and single-colour (399 nm) photoionisation and their efficiency is compared to electron impact ionisation. Nearly deterministic loading of a desired number of YbII ions into a linear Paul trap is demonstrated.Comment: 9 pages. Considerably extended and revised version including new dat

Detecting early kidney damage in horses with colic by measuring matrix metalloproteinase -9 and -2, other enzymes, urinary glucose and total proteins

BACKGROUND: The aim of the study was to investigate urine matrix metalloproteinase (MMP-2 and -9) activity, alkaline phosphatase/creatinine (U-AP/Cr) and gamma-glutamyl-transpeptidase/creatinine (U-GGT/Cr) ratios, glucose concentration, and urine protein/creatinine (U-Prot/Cr) ratio and to compare data with plasma MMP-2 and -9 activity, cystatin-C and creatinine concentrations in colic horses and healthy controls. Horses with surgical colic (n = 5) were compared to healthy stallions (n = 7) that came for castration. Blood and urine samples were collected. MMP gelatinolytic activity was measured by zymography. RESULTS: We found out that horses with colic had significantly higher urinary MMP-9 complex and proMMP-9 activities than horses in the control group. Colic horses also had higher plasma MMP-2 activity than the control horses. Serum creatinine, although within reference range, was significantly higher in the colic horses than in the control group. There was no significant increase in urinary alkaline phosphatase, gamma-glutamyltranspeptidase or total proteins in the colic horses compared to the control group. A human cystatin-C test (Dako Cytomation latex immunoassay(® )based on turbidimetry) did not cross react with equine cystatin-C. CONCLUSION: The results indicate that plasma MMP-2 may play a role in the pathogenesis of equine colic and urinary MMP-9 in equine kidney damage

Performance and characterization of the FinEsuseAMS beamline at the MAX IV Laboratory

FinEstBeAMS (Finnish-Estonian Beamline for Atmospheric and Materials Sciences) is a multidisciplinary beamline constructed at the 1.5 GeV storage ring of the MAX IV synchrotron facility in Lund, Sweden. The beamline covers an extremely wide photon energy range, 4.5-1300 eV, by utilizing a single elliptically polarizing undulator as a radiation source and a single grazingincidence plane grating monochromator to disperse the radiation. At photon energies below 70 eV the beamline operation relies on the use of optical and thin-film filters to remove higher-order components from the monochromated radiation. This paper discusses the performance of the beamline, examining such characteristics as the quality of the gratings, photon energy calibration, photon energy resolution, available photon flux, polarization quality and focal spot size