34 research outputs found
Flow Injection Analysis of Hydrogen Peroxide with Peroxyoxalate Chemiluminescence Detection
This study reports a new, rapid and sensitive flow injection analysis (FIA) with peroxyoxalate chemiluminescence detection (PO-CL) for determination of hydrogen peroxide through merging zone principle. Di (N-Succinimidyl) oxalate was applied for the first time as peroxyoxalate chemiluminescence reagent. The CL was produced by the oxidation of Di (N-Succinimidyl) oxalate by hydrogen peroxide in the presence of a fluorescent compound, (9, 10 Bis phenyl ethynyl anthracene) and imidazole as a catalyst. Various parameters associated with this flow system were studied and essential optimizations were carried out. Calibration graph was constructed for determination of hydrogen peroxide in the range (0.02-0.34 mol.L–1) with correlation coefficient (R2) (0.982).The method was applied successfully for the determination of hydrogen peroxide in commercial pharmaceutical products and in tap water
Evaluation of oxidative stress in Vigna radiata L. in response to chlorpyrifos
This study aims to evaluate the effect of chlorpyrifos on several
metabolic and stress related parameters of Vigna radiata L.
Twenty-days-old plants were exposed to several concentrations of
chlorpyrifos, ranging from 0 – 1.5 mM through foliar spray in the
field condition. Analyses were done at pre flowering (Day 5), flowering
(Day 10) and post flowering (Day 20) stages after the treatment. Lipid
peroxidation rate, proline, dehydroascorbate, oxidized and total
glutathione were all ascended. Chlorpyrifos enhanced lipid peroxidation
rate and proline content with 1.5 mM at day 20 whereas
dehydroascorbate, oxidized and total glutathione were increased in 1.5
mM at day 10. However, dose dependence significantly declined in
content of ascorbate and reduced glutathione levels were observed at
all growth stages. Among the enzymatic antioxidants, activities of
superoxide dismutase, ascorbate peroxidase and glutathione reductase
enhanced significantly in all the concentrations at day 10. Maximum
catalase activity was observed at day 10 in control and it declined
thereafter. The above results clearly depicted the provoked state of
oxidative stress under chlorpyrifos exposure in Vigna radiata L. and
therefore can be used to evaluate the degree of insecticide
contamination to plant which may be serving as biomarker in Vigna
radiata L
Saturated fatty acid regulated lncRNA dataset during in vitro human embryonic neurogenesis
Human embryonic stem cells (hESCs) were used as a model of embryonic neurogenesis to identify the effect of excess fat uptake on neurodevelopment (Ardah et al., 2018). Herein, by directed differentiation of hESCs into neurons using established protocols, this data was generated for expression profiles of select lncRNAs during in vitro embryonic neurogenesis and their differential expression due to excess fat (palmitate) uptake. The undifferentiated hESCs were treated with 250 µM palmitate after identifying it as the highest concentration which is non-toxic to these cells. The palmitate treated hESCs were differentiated towards neurons keeping the levels of palmitate consistent throughout the differentiation process and fat uptake was confirmed by Oil Red O staining. The expression analysis of lncRNAs was performed by RT-qPCR on vehicle control and palmitate treated cells from 4 stages of differentiation, D0 (undifferentiated hESCs), D12 (neural stem cells), D44 (neural progenitors) and D70 (neurons) using lncRNAs array plates from Arraystar Inc. which contains 372 functionally identified lncRNAs found to be associated with lipid metabolism and other pathways (Cat# AS-NR-004)
Higher O-GlcNAc Levels Are Associated with Defects in Progenitor Proliferation and Premature Neuronal Differentiation during in-Vitro Human Embryonic Cortical Neurogenesis
The nutrient responsive O-GlcNAcylation is a dynamic post-translational protein modification found on several nucleocytoplasmic proteins. Previous studies have suggested that hyperglycemia induces the levels of total O-GlcNAcylation inside the cells. Hyperglycemia mediated increase in protein O-GlcNAcylation has been shown to be responsible for various pathologies including insulin resistance and Alzheimer's disease. Since maternal hyperglycemia during pregnancy is associated with adverse neurodevelopmental outcomes in the offspring, it is intriguing to identify the effect of increased protein O-GlcNAcylation on embryonic neurogenesis. Herein using human embryonic stem cells (hESCs) as model, we show that increased levels of total O-GlcNAc is associated with decreased neural progenitor proliferation and premature differentiation of cortical neurons, reduced AKT phosphorylation, increased apoptosis and defects in the expression of various regulators of embryonic corticogenesis. As defects in proliferation and differentiation during neurodevelopment are common features of various neurodevelopmental disorders, increased O-GlcNAcylation could be one mechanism responsible for defective neurodevelopmental outcomes in metabolically compromised pregnancies such as diabetes
Nutrient sensitive protein O-GlcNAcylation modulates the transcriptome through epigenetic mechanisms during embryonic neurogenesis
10.26508/lsa.202201385LIFE SCIENCE ALLIANCE58completedcomplete