Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme.

A major mechanism of mRNA decay occurs by the process of deadenylation, decapping and 5' --> 3' exonucleolytic degradation. Recently, the product of the DCP1 gene has been shown to be required for decapping mRNAs in vivo and co-purifies with decapping activity in vitro. We have purified Dcp1p to homogeneity and shown that it is sufficient for decapping, thereby indicating that Dcp1p is the decapping enzyme. Characterization of Dcp1p activity in vitro indicated that the 7-methyl group of the cap structure contributes to the enzyme's substrate specificity. In addition, Dcp1p was effectively inhibited by uncapped mRNAs, and the enzyme efficiently cleaved substrates that were >/=25 nucleotides in length, with a preference for longer mRNA substrates. These properties suggest that Dcp1p recognizes the mRNA substrate by interactions with both the cap and the RNA moiety. The Dcp1p is also a phosphoprotein, suggesting its activity may be regulated by post-transcriptional modification

Perceiving musical identity: performer identification is dependent on performer expertise and expressiveness, but not on listener expertise

Can listeners distinguish unfamiliar performers playing the same piece on the same instrument? Professional performers recorded two expressive and two inexpressive interpretations of a short organ piece. Nonmusicians and musicians listened to these recordings and grouped together excerpts they thought had been played by the same performer. Both musicians and nonmusicians performed significantly above chance. Expressive interpretations were sorted more accurately than inexpressive ones, indicating that musical individuality is communicated more efficiently through expressive performances. Furthermore, individual performers’ consistency and distinctiveness with respect to expressive patterns were shown to be excellent predictors of categorisation accuracy. Categorisation accuracy was superior for prize-winning performers compared to non-winners, suggesting a link between performer competence and the communication of musical individuality. Finally, results indicate that temporal information is sufficient to enable performer recognition, a finding that has broader implications for research on the detection of identity cues

Valuing Value Plays with Bloomberg

International audienceValue analysts attempt to identify particularly undervalued securities in an attempt to produce positive returns and even positive alpha. This paper traces the process of that pursuit from company selection through valuation. The resource showcased throughout is the tool used by professional portfolio managers and security analysts – Bloomberg. Each iteration along the process is demonstrated through Bloomberg functions and screenshots, providing a step by step map for identifying potential value plays