6 research outputs found
Claudin-binder C-CPE mutants enhance permeability of insulin across human nasal epithelial cells
Histone deacetylase inhibition prevents cell death induced by loss of tricellular tight junction proteins in temperature-sensitive mouse cochlear cells
JAM-A as a prognostic factor and new therapeutic target in multiple myeloma
Cell adhesion in the multiple myeloma (MM) microenvironment has been recognized as a major mechanism of MM cell survival and
the development of drug resistance. Here we addressed the hypothesis that the protein junctional adhesion molecule-A (JAM-A)
may represent a novel target and a clinical biomarker in MM. We evaluated JAM-A expression in MM cell lines and in 147 MM
patient bone marrow aspirates and biopsies at different disease stages. Elevated JAM-A levels in patient-derived plasma cells were
correlated with poor prognosis. Moreover, circulating soluble JAM-A (sJAM-A) levels were significantly increased in MM patients as
compared with controls. Notably, in vitro JAM-A inhibition impaired MM migration, colony formation, chemotaxis, proliferation and
viability.In vivo treatment with an anti-JAM-A monoclonal antibody (αJAM-A moAb) impaired tumor progression in a murine
xenograft MM model. These results demonstrate that therapeutic targeting of JAM-A has the potential to prevent MM progression,
and lead us to propose JAM-A as a biomarker in MM, and sJAM-A as a serum-based marker for clinical stratification