Multi-cluster dynamics in Λ13C^{13}_\Lambda{\rm C} and analogy to clustering in 12C^{12}{\rm C}

We investigate structure of $^{13}_\Lambda{\rm C}$ and discuss the difference and similarity between the structures of $^{12}{\rm C}$ and $^{13}_\Lambda{\rm C}$ by answering the questions if the linear-chain and gaslike cluster states, which are proposed to appear in $^{12}{\rm C}$, survives, or new structure states appear or not. We introduce a microscopic cluster model called, Hyper-Tohsaki-Horiuchi-Schuck-R\"opke (H-THSR) wave function, which is an extended version of the THSR wave function so as to describe $\Lambda$ hypernuclei. We obtained two bound states and two resonance (quasi-bound) states for $J^\pi=0^+$ in $^{13}_\Lambda{\rm C}$, corresponding to the four $0^+$ states in $^{12}{\rm C}$. However, the inversion of level ordering between the spectra of $^{12}{\rm C}$ and $^{13}_\Lambda{\rm C}$, i.e. that the $0_3^+$ and $0_4^+$ states in $^{13}_\Lambda{\rm C}$ correspond to the $0_4^+$ and $0_3^+$ states in $^{12}{\rm C}$, respectively, is shown to occur. The additional $\Lambda$ particle reduces sizes of the $0_2^+$ and $0_3^+$ states in $^{13}_\Lambda{\rm C}$ very much, but the shrinkage of the $0_4^+$ state is only a half of the other states. In conclusion, the Hoyle state becomes quite a compact object with ${^{9}_\Lambda{\rm Be}}+\alpha$ configuration in $^{13}_\Lambda{\rm C}$ and is no more gaslike state composed of the $3\alpha$ clusters. Instead, the $0_4^+$ state in $^{13}_\Lambda{\rm C}$, coming from the $^{12}{\rm C}(0_3^+)$ state, appears as a gaslike state composed of $\alpha+\alpha+^{5}_\Lambda{\rm He}$ configuration, i.e. the Hoyle analog state. A linear-chain state in a $\Lambda$ hypernucleus is for the first time predicted to exist as the $0_3^+$ state in $^{13}_\Lambda{\rm C}$ with more shrunk arrangement of the $3\alpha$ clusters along $z$-axis than the $3\alpha$ linear-chain configuration realized in the $^{12}{\rm C}(0_4^+)$ state.Comment: 9 pages, 6 figures, figures rearranged, accepted for publication in PL

PROPOSAL OF AN ADDITIONAL-INERTIA TUNING METHOD TO VISCOUS LOAD FOR HIGH SPEED MOTION TRAINING

This research proposes a new training system for high speed motions. In the proposed training system, a relatively small inertia is added to a viscous load in order to increase the load without decreasing the maximum speed. Moreover, in order to increase energy consumption of humans, we artificially make the additional inertia zero during motions. Consequently, the additional inertia is tuned to increase the load torque and the energy with keeping the maximum speed. The effectiveness of the proposed system is verified by some experimental results

Probing ΞN\Xi N interaction through inversion of spin-doublets in ΞNαα\Xi N \alpha \alpha nuclei

A new way to study the spin-isospin dependence of the $\Xi N$ interaction is explored through the energy levels of $\Xi N\alpha$ and $\Xi N\alpha \alpha$ systems with $\alpha$ being a spectator to attract the $\Xi N$ pair without changing its spin-isospin structure. By using the Gaussian expansion method (GEM) with the state-of-the-art $\Xi N$ potential obtained from lattice QCD calculations, it is found that $\Xi N\alpha \alpha$ has spin-doublet bound states with $J^{\pi}={1^-}$ and $2^-$ in both isospin triplet and singlet channels. The inversion of the $1^-$-$2^-$ spin-doublet between the iso-triplet and the iso-singlet is found to be strongly correlated with the relative strengths of the $\Xi N$ interaction in the $^{11}{\rm S}_0, ^{13}{\rm S}_1,^{31}{\rm S}_0$ and $^{33}{\rm S}_1$ channels. The $(K^-, K^+)$ and $(K^-,K^0)$ reactions on the $^{10}$B target are proposed to produce those bound states.Comment: 5pages, 5figure

A case of wound dual infection with Pasteurella dagmatis and Pasteurella Canis resulting from a dog bite - limitations of Vitek-2 system in exact identification of Pasteurella species

<p>Abstract</p> <p>Background</p> <p><it>Pasteurella </it>species, widely known as indigenous orgganisms in the oral and gastrointestinal floras of many wild and domestic animals, are important pathogens in both animals and humans. Human infections due to <it>Pasteurella </it>species are in most cases associated with infected injuries following animal bites. We encountered a rare case of dual infections caused by different two <it>Pasteurella </it>species occurred in a previously healthy 25-year-old female sustaining injury by a dog-bite.</p> <p>Methodology</p> <p>Exudates from the open wound of her dog-bite site, together with the saliva of the dog were submitted for bacteriological examination. Predominantly appearing grayish-white smooth colonies with almost the same colonial properties but slightly different glistening grown on chocolate and sheep blood agar plates were characterized morphologically by Gram's stain, biochemically by automated instrument using Vitek 2 system using GN cards together with commercially available kit system, ID-Test HN-20 rapid panels, and genetically by sequencing the 16S rRNA genes of the organism using a Taq DyeDeoxy Terminator Cycle Sequencing and a model 3100 DNA sequencer instrument.</p> <p>Results</p> <p>The causative isolates from the dog-bite site were finally identified as <it>P</it>. <it>canis </it>and <it>P</it>. <it>dagmatis </it>from the findings of the morphological, cultural, and biochemical properties together with the comparative sequences of the 16S rRNA genes. Both the isolates were highly susceptible to many antibiotics and the patient was successfully treated with the administration of so-called the first generation cephalosporin, cefazolin followed by so-called the third generation cephalosporin, cefcapene pivoxil. The isolate from the dog was subsequently identified as <it>P</it>. <it>canis</it>, the same species as the isolate from the patient.</p> <p>Conclusions</p> <p>To the best of our knowledge, this was the second report of a dual infection with <it>Pasteurella </it>species consisting of <it>P</it>. <it>dagmatis </it>and <it>P. canis </it>resulting from a dog-bite, followed by the first report of dual infections due to <it>P</it>. <it>dagmatis </it>and <it>P. multocida </it>in 1988. Our isolate finally identified as <it>P</it>. <it>dagmatis </it>was misidentified as <it>P</it>. <it>pneumotripica by </it>means of the Vitek 2 system. The species name "<it>P</it>. <it>dagmatis" </it>was not included in the database of the system. It is also important for routine clinical microbiology laboratories to know the limitation of the automated Vitek 2 system for the accurate identification of <it>Pasteurella </it>species especially <it>P</it>. <it>dagmatis</it>. It should be emphasized that there still exists much room for improvement in Vitek 2 system. Significant improvement of Vitek 2 system especially in the identification of <it>Pasteurella </it>species is urgently desired.</p

Clinical significance of VEGF-A, -C and -D expression in esophageal malignancies

Vascular endothelial growth factors ( VEGF)- A, - C and - D are members of the proangiogenic VEGF family of glycoproteins. VEGF-A is known to be the most important angiogenic factor under physiological and pathological conditions, while VEGF-C and VEGF-D are implicated in the development and sprouting of lymphatic vessels, so called lymphangiogenesis. Local tumor progression, lymph node metastases and hematogenous tumor spread are important prognostic factors for esophageal carcinoma ( EC), one of the most lethal malignancies throughout the world. We found solid evidence in the literature that VEGF expression contributes to tumor angiogenesis, tumor progression and lymph node metastasis in esophageal squamous cell carcinoma ( SCC), and many authors could show a prognostic value for VEGF-assessment. In adenocarcinoma (AC) of the esophagus angiogenic properties are acquired in early stages, particularly in precancerous lesions like Barrett's dysplasia. However, VEGF expression fails to give prognostic information in AC of the esophagus. VEGF-C and VEGF-D were detected in SCC and dysplastic lesions, but not in normal mucosa of the esophagus. VEGF-C expression might be associated with lymphatic tumor invasion, lymph node metastases and advanced disease in esophageal SCC and AC. Therapeutic interference with VEGF signaling may prove to be a promising way of anti-angiogenic co-treatment in esophageal carcinoma. However, concrete clinical data are still pending

Mass Transfer by Stellar Wind

I review the process of mass transfer in a binary system through a stellar wind, with an emphasis on systems containing a red giant. I show how wind accretion in a binary system is different from the usually assumed Bondi-Hoyle approximation, first as far as the flow's structure is concerned, but most importantly, also for the mass accretion and specific angular momentum loss. This has important implications on the evolution of the orbital parameters. I also discuss the impact of wind accretion, on the chemical pollution and change in spin of the accreting star. The last section deals with observations and covers systems that most likely went through wind mass transfer: barium and related stars, symbiotic stars and central stars of planetary nebulae (CSPN). The most recent observations of cool CSPN progenitors of barium stars, as well as of carbon-rich post-common envelope systems, are providing unique constraints on the mass transfer processes.Comment: Chapter 7, in Ecology of Blue Straggler Stars, H.M.J. Boffin, G. Carraro & G. Beccari (Eds), Astrophysics and Space Science Library, Springe

Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos

hybridization. hybridization (WISH).Here we describe a modification to the WISH protocol that is essential to prevent DNA cross-hybridization and to specifically detect transgene mRNA transcripts in electroporated embryos. Our optimized WISH procedure can be applied not only to electroporated chick embryos but also to other embryos or adult tissues that have been transfected with large amounts of reporter- or expression construct DNA

Resequencing of genes for transforming growth factor β1 (TGFB1) type 1 and 2 receptors (TGFBR1, TGFBR2), and association analysis of variants with diabetic nephropathy

BACKGROUND: Diabetic nephropathy is the leading cause of end stage renal failure in the western world. There is substantial epidemiological evidence supporting a genetic predisposition to diabetic nephropathy, however the exact molecular mechanisms remain unknown. Transforming growth factor (TGFβ1) is a crucial mediator in the pathogenesis of diabetic nephropathy. METHODS: We investigated the role of five known single nucleotide polymorphisms (SNPs) in the TGFB1 gene for their association with diabetic nephropathy in an Irish, type 1 diabetic case (n = 272) control (n = 367) collection. The activity of TGFβ1 is facilitated by the action of type 1 and type 2 receptors, with both receptor genes (TGFBR1 and TGFBR2) shown to be upregulated in diabetic kidney disease. We therefore screened TGFBR1 and TGFBR2 genes for genomic variants using WAVE™ (dHPLC) technology and confirmed variants by direct capillary sequencing. Allele frequencies were determined in forty-eight healthy individuals. Data for all SNPs was assessed for Hardy Weinberg equilibrium, with genotypes and allele frequencies compared using the χ(2 )test for contingency tables. Patterns of linkage disequilibrium were established and common haplotypes estimated. RESULTS: Fifteen variants were identified in these genes, seven of which are novel, and putatively functional SNPs were subsequently genotyped using TaqMan™, Invader™ or Pyrosequencing(® )technology. No significant differences (p > 0.1) were found in genotype or allele distributions between cases and controls for any of the SNPs assessed. CONCLUSION: Our results suggest common variants in TGFB1, TGFBR1 and TGFBR2 genes do not strongly influence genetic susceptibility to diabetic nephropathy in an Irish Caucasian population