The Translation Initiation Factor 3f (eIF3f) Exhibits a Deubiquitinase Activity Regulating Notch Activation

The translation initiation factor complex eIF3f has an intrinsic deubiquitinase activity and regulates the Notch signaling pathway

Colon Capsule Endoscopy compared to Conventional Colonoscopy under routine screening conditions

Colonoscopy (CSPY) for colorectal cancer screening has several limitations. Colon Capsule Endoscopy (PillCam Colon, CCE) was compared to CSPY under routine screening conditions

The Functions of Auxilin and Rab11 in Drosophila Suggest That the Fundamental Role of Ligand Endocytosis in Notch Signaling Cells Is Not Recycling

Notch signaling requires ligand internalization by the signal sending cells. Two endocytic proteins, epsin and auxilin, are essential for ligand internalization and signaling. Epsin promotes clathrin-coated vesicle formation, and auxilin uncoats clathrin from newly internalized vesicles. Two hypotheses have been advanced to explain the requirement for ligand endocytosis. One idea is that after ligand/receptor binding, ligand endocytosis leads to receptor activation by pulling on the receptor, which either exposes a cleavage site on the extracellular domain, or dissociates two receptor subunits. Alternatively, ligand internalization prior to receptor binding, followed by trafficking through an endosomal pathway and recycling to the plasma membrane may enable ligand activation. Activation could mean ligand modification or ligand transcytosis to a membrane environment conducive to signaling. A key piece of evidence supporting the recycling model is the requirement in signaling cells for Rab11, which encodes a GTPase critical for endosomal recycling. Here, we use Drosophila Rab11 and auxilin mutants to test the ligand recycling hypothesis. First, we find that Rab11 is dispensable for several Notch signaling events in the eye disc. Second, we find that Drosophila female germline cells, the one cell type known to signal without clathrin, also do not require auxilin to signal. Third, we find that much of the requirement for auxilin in Notch signaling was bypassed by overexpression of both clathrin heavy chain and epsin. Thus, the main role of auxilin in Notch signaling is not to produce uncoated ligand-containing vesicles, but to maintain the pool of free clathrin. Taken together, these results argue strongly that at least in some cell types, the primary function of Notch ligand endocytosis is not for ligand recycling

Knowledge base to facilitate anthropogenic resource assessment

The traditional mining sector uses resource assessments to estimate the mineability of natural resources. The results are communicated to investors, authorities and corporate management boards in a standardized manner, at least on a country level. The recycling sector also requires estimates of recoverable anthropogenic resources. Evidence-based resource assessment, including the selection of parameters for characterising resources and methods for assessing their recoverability, is essential to obtain comparable estimates over time and across scales. Within this report, the COST Action MINEA presents a practical and user-friendly knowledge base for facilitating anthropogenic resource assessments. The fouces is on extractives industry residues, residues in landfills, residues from municipal solid waste incineration as well as construction & demolition waste flows. The key objectives are: To relate current knowledge levels, gaps and future needs to assessments of viability of anthropogenic resource recovery. To review case studies that demonstrate anthropogenic resource assessment in combination with resource classification in order to communicate the viability of anthropogenic resource recovery. We encourage academics, businesses and government organisations to use this report for: designing and developing case studies, future planning, developing standards for characterizing resource quantities and evaluating their recoverability, and collecting and harmonizing resource statistics. ************* The “Mining the European Anthroposphere” (MINEA) is a pan-European expert network, which received funding from the COST Association between 2016 and 2020. The network pools knowledge for estimating the future recoverability of raw materials from anthropogenic resources

Research on NLP for RE at Fraunhofer FKIE: A report on grouping requirements

In this report we describe the previous research done by our institute in the field of requirement analysis using different natural language processing methods. To represent the different degrees of similarity between words we implemented different methods that make use of synonyms and hyperonyms. We present the strengths of our methods and identify their weaknesses. For our future research we want to incorporate Word Embeddings as they solve most of the difficulties we faced with synonyms and hyperonyms. Copyright c 2018 by the paper's authors