Are you worth it? Can you fix it? Investigating the sustainability of mundane activities using theories of everyday practice and human/ object interactions

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Novel splice variants of Rat CaV2.1 that lack much of the synaptic protein interaction site are expressed in neuroendocrine cells

Voltage-gated Ca(2+) channels are responsible for the activation of the Ca(2+) influx that triggers exocytotic secretion. The synaptic protein interaction (synprint) site found in the II-III loop of Ca(V)2.1 and Ca(V)2.2 mediates a physical association with synaptic proteins that may be crucial for fast neurotransmission and axonal targeting. We report here the use of nested PCR to identify two novel splice variants of rat Ca(V)2.1 that lack much of the synprint site. Furthermore, we compare immunofluorescence data derived from antibodies directed against sequences in the Ca(V)2.1 synprint site and carboxyl terminus to show that channel variants lacking a portion of the synprint site are expressed in two types of neuroendocrine cells. Immunofluorescence data also suggest that such variants are properly targeted to neuroendocrine terminals. When expressed in a mammalian cell line, both splice variants yielded Ca(2+) currents, but the variant containing the larger of the two deletions displayed a reduced current density and a marked shift in the voltage dependence of inactivation. These results have important implications for Ca(V)2.1 function and for the mechanisms of Ca(V)2.1 targeting in neurons and neuroendocrine cells

Modelling low-velocity impact damage and compression after impact of 3D woven structures considering compaction

A novel finite element modelling approach is presented which incorporates representative binder yarn compaction, for simulating the low-velocity impact (LVI) and compression after impact (CAI) response of 3D woven layer-to-layer carbon/epoxy composite architectures. Simulations of out-of-plane drop-weight impact tests were performed at energies of 32 J and 42 J. Warp and weft layers were modelled as continuous plies and three different approaches were explored to model the binder reinforcement; (i) with a rectangular cross-section and non-compacted, (ii) with an elliptical cross-section and non-compacted, and (iii) an elliptical cross-section which accounts for compaction. Predictions were compared with experimental results from literature and it is shown that modelling the binder reinforcement as an elliptical cross-section with compaction leads to a predicted damage area, on the impacted side, which is within 2%, and the non-impacted side within 6% of experimental measurements. The predicted CAI strength is within 11% of the experimental values. </p