28 research outputs found
Covalent enzyme coupling on cellulose acetate membranes for glucose sensor development
Get PDFInternational audienceMethods for immobilizing glucose oxidase (GOx) on cellulose acetate (CA) membranes are compared. The optimal method involves covalent coupling of bovine serum albumin (BSA) to CA membrane and a subsequent reaction of the membrane with GOx, which has previously been activated with an excess of p-benzoquinone. This coupling procedure is fairly reproducible and allows the preparation of thin membranes (5-20 µm) showing high surface activities (1-3 U/cm2) which are stable over a period of 1-3 months. Electrochemical and radiolabeling experiments show that enzyme inactivation as a result of immobilization is negligible. A good correlation between surface activity of membranes and their GOx load is observed
Theory for double potential step chronoamperometry, chronocoulometry, and chronoabsorptometry with a quasi-reversible electrode reaction
No full textSimultaneous electrochemical determination of diffusion and partition coefficients of potassium ferrocyanide for albumin-glutaraldehyde membranes
No full textDynamic and steady-state response of electrochemical detectors based on arrays of small electrodes
No full textEffect of chemical seed treatment on the germination and seedling growth of canola under different soil conditions
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