MOESM1 of Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production in Aspergillus niger

Additional file 1: Figure S1. Design of acl1 and acl2 expression cassette. Figure S2. Shake flask cultivation of CitB#99 ACL G1 Z. Table S1. Relative copy numbers of introduced acl12 and IA titers achieved in shake flask cultivations. Figure S3. Southern-Blot results of selected CitB#99-acl transformants. Table S2. Carbon balance of 10L fed-batch bioreactor cultivation of ACL G1 Z. Figure S4. Dissolved oxygen profile and pH profile during 10 L controlled fed-batch cultivation of strain CitB#99-ACL G1 Z. Figure S5. Total nitrogen concentration during 10 L controlled fed-batch cultivation of ACL G1 Z. Figure S6. Repeat fed-batch cultivation of ACL G1 Z performed in 5 L BioFlo 320 (Eppendorf) controlled bioreactors. Table S3. Carbon balance of repeat fed-batch 5 L bioreactor cultivation of ACL G1 Z