93 research outputs found
Biogenesis of the polymeric IgA receptor in rat hepatocytes. II. Localization of its intracellular forms by cell fractionation studies.
Import of rat ornithine transcarbamylase precursor into mitochondria: two-step processing of the leader peptide.
ARF GTPases and their GEFs and GAPs: concepts and challenges
Detailed structural, biochemical, cell biological, and genetic studies of any gene/protein are required to develop models of its actions in cells. Studying a protein family in the aggregate yields additional information, as one can include analyses of their coevolution, acquisition or loss of functionalities, structural pliability, and the emergence of shared or variations in molecular mechanisms. An even richer understanding of cell biology can be achieved through evaluating functionally linked protein families. In this review, we summarize current knowledge of three protein families: the ARF GTPases, the guanine nucleotide exchange factors (ARF GEFs) that activate them, and the GTPase-activating proteins (ARF GAPs) that have the ability to both propagate and terminate signaling. However, despite decades of scrutiny, our understanding of how these essential proteins function in cells remains fragmentary. We believe that the inherent complexity of ARF signaling and its regulation by GEFs and GAPs will require the concerted effort of many laboratories working together, ideally within a consortium to optimally pool information and resources. The collaborative study of these three functionally connected families ( \u3e /=70 mammalian genes) will yield transformative insights into regulation of cell signaling
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Pulmonary Carcinoid Surface Receptor Modulation Using Histone Deacetylase Inhibitors
Study describes the use of a non-cytotoxic dose of histone deacetylase (HDAC) inhibitors to induce pulmonary carcinoid somatostatin receptor subtype 2 (SSTR2) expression to diagnose pulmonary carcinoids
Exocytic transport vesicles generated in vitro from the trans-Golgi network carry secretory and plasma membrane proteins.
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