Modelling Alzheimer-like cognitive deficits in rats using biperiden as putative cognition impairer

To enable the development of effective treatments for dementias such as Alzheimer’s disease (AD), it is important to establish valid animal models of cognitive impairments. Scopolamine is widely used to induce cognitive deficits in animal models of AD, but also causes non-cognitive side effects. We assessed whether biperiden, a selective antagonist of M1 muscarinic receptors, which are predominantly expressed in brain areas involved in cognitive processes, causes cognitive deficits without inducing peripheral side-effects. Two different doses of biperiden (3 or 10 mg kg−1) on the acquisition of a spatial cone field task were assessed in male Lister Hooded rats. This task measures, among others, spatial working (WM) – and reference memory (RM) simultaneously. Biperiden did not impair learning of the task. The animals reached asymptotic levels for all variables except reference memory and the number of rewards collected. However, the 10 mg kg−1 dose decreased the tendency of rats to use searching strategies to solve the task and made them slower to start searching and completing the task. In conclusion, though no effects on WM and RM performance were seen, the present study cannot conclude that biperiden acts as a more selective cognition impairer than scopolamine in other rats strains and/or other doses than those tested

Modelling Alzheimer-like cognitive deficits in rats using biperiden as putative cognition impairer.

peer reviewedTo enable the development of effective treatments for dementias such as Alzheimer's disease (AD), it is important to establish valid animal models of cognitive impairments. Scopolamine is widely used to induce cognitive deficits in animal models of AD, but also causes non-cognitive side effects. We assessed whether biperiden, a selective antagonist of M1 muscarinic receptors, which are predominantly expressed in brain areas involved in cognitive processes, causes cognitive deficits without inducing peripheral side-effects. Two different doses of biperiden (3 or 10mgkg(-1)) on the acquisition of a spatial cone field task were assessed in male Lister Hooded rats. This task measures, among others, spatial working (WM) - and reference memory (RM) simultaneously. Biperiden did not impair learning of the task. The animals reached asymptotic levels for all variables except reference memory and the number of rewards collected. However, the 10mgkg(-1) dose decreased the tendency of rats to use searching strategies to solve the task and made them slower to start searching and completing the task. In conclusion, though no effects on WM and RM performance were seen, the present study cannot conclude that biperiden acts as a more selective cognition impairer than scopolamine in other rats strains and/or other doses than those tested

Modelling Alzheimer-like cognitive deficits in rats using biperiden as putative cognition impairer

To enable the development of effective treatments for dementias such as Alzheimer’s disease (AD), it is important to establish valid animal models of cognitive impairments. Scopolamine is widely used to induce cognitive deficits in animal models of AD, but also causes non-cognitive side effects. We assessed whether biperiden, a selective antagonist of M1 muscarinic receptors, which are predominantly expressed in brain areas involved in cognitive processes, causes cognitive deficits without inducing peripheral side-effects. Two different doses of biperiden (3 or 10 mg kg−1) on the acquisition of a spatial cone field task were assessed in male Lister Hooded rats. This task measures, among others, spatial working (WM) – and reference memory (RM) simultaneously. Biperiden did not impair learning of the task. The animals reached asymptotic levels for all variables except reference memory and the number of rewards collected. However, the 10 mg kg−1 dose decreased the tendency of rats to use searching strategies to solve the task and made them slower to start searching and completing the task. In conclusion, though no effects on WM and RM performance were seen, the present study cannot conclude that biperiden acts as a more selective cognition impairer than scopolamine in other rats strains and/or other doses than those tested

Testing the potential of the Sow Stance Information System (SowSIS) based on a force plate system built into an electronic sow feeder for on-farm automatic lameness detection in breeding sows

peer reviewedLameness is a common problem in breeding sows, which often goes undetected for long periods with severe consequences for animal welfare and farm productivity. Automatic lameness detection could help pig farmers to recognise and treat lameness sooner. The SowSIS (Sow Stance Information System) is a device consisting of four force plates and providing non-invasive force measurements per leg of the sow. In this study, the SowSIS was built into electronic sow feeders and validated for lameness detection in group-housed gestating sows. Data was automatically collected for 71 sows. Visual gait scoring was performed twice a week using a 150-mm tagged visual analogue scale to determine the sows' lameness status. Only data from 32 gait scoring days were included, adding up to 674 sow days. A sow was classified as lame using >60 mm as the cut-off value for the visual gait scores. Stance variables were calculated from the SowSIS data per sow per day. First, a multivariable linear mixed model was used to detect lameness, using stance variables with significant influence on the gait score. The model's performance was 78.5% sensitivity, 81.4% specificity, 80.7% accuracy, 57.4% lame predictive value and 92.2% non-lame predictive value. Second, five types of classification models were tested to determine the lame leg on a sub-dataset. The random forest model could predict the lame leg correctly 90% of the time. The SowSIS shows great promise as an on-farm lameness detection system, as it allows continuous non-invasive data collection in a practical setting