Сучасний погляд «на обман, що розтягнувся на віки»

Сингаївська, І. В. Сучасний погляд «на обман, що розтягнувся на віки» / І. В. Сингаївська // Вісник Національного університету внутрішніх справ. - 2004. – Вип. 27. - С. 38-43Проведений історичний екскурс та сучасна характеристика фальшивомонетництва дозволила переконатись, що способи та види підробки набувають якості, масовості та широкого використання новітньої комп’ютерно-копіювальної техніки. Проведенный исторический экскурс и современная характеристика фальшивомонетничества позволила убедиться, что способы и виды подделки приобретают качество, массовость и широкое использование новейшей компьютерно-копировальной техники. The historical excursion and the modern counterfeiting characteristic made it possible to verify that the methods and types of counterfeiting acquire quality, mass character and widespread use of the latest computer copying equipment

Microanalysis of magnetic structure of yttrium-iron garnet films by using the scanning probe microscopy methods

In this paper, the features of the microstructure of magnetic domains observed in ferrite-garnet films (FGF) have been presented. The studied FGF with orientation (111) were grown on gallium-gadolinium substrate by using liquid-phase epitaxy. The study of distribution inherent to magnetic domains was carried out using magnetic force microscopy (MFM) with the scanning probe microscope NanoScope IIIa Dimension 3000TM. In the course of these researches, optimization of the MFM method was carried out to obtain high-quality and correct images of magnetic domains in FGF. Nanorelief and magnetic microstructure of FGF surface were studied, depending on their thickness, on external magnetic field and doses of boron ion implantation. For these objects, it was established that stripe domain structure is characteristic, the period of which depends on the film thickness. The nature of transformation of domain structure depending on thickness is close to that theoretically predictable at low thicknesses (up to 10 µm). Nanorelief of film surfaces is virtually unchanged depending on thickness. An external magnetic field with the magnitude 4 mT causes significant changes in domain configuration and allows to visualize heterogeneity of magnetic structure. Ion implantation leads to a slight smoothing of nanorelief films (roughness of 0.2 nm) and to more accurate displaying the magnetic microstructure, which is associated with processes of structural ordering under ionic bombardment

Asmeninių vartotojo savybių įtaka „žaliajam“ vartojimui: moderuojantis įmonių socialinės atsakomybės suvokimo vaidmuo.

The Impact of Personal Characteristics on Green Consumption Behavior: the Moderating Effect of Perception of Corporate Social Responsibility

Sensing metal ions-peptide and protein interfacial interactions

Metal ions play an important role in vital biochemical processes in the human body, interacting with peptides and proteins. It is known that too low as well as too high concentrations of metal ions can cause various health problems, damaging kidneys, bones, and/or the liver. The imbalance of metal ions is also associated with the development of neurodegenerative diseases, such as Alzheimer's and Parkinson's. Therefore, monitoring concentrations of metal ions and understanding their complexation mechanisms with peptides and proteins opens possibilities for early diagnosis and eventually for the development of a cure. In this work, we first demonstrate how peptides glycyl-glycyl-histidine (GGH) and Oxytocin (OT) can help for the selective detection of metal ions in potentiometric sensing devices. The GGH peptide selectively binds Cu2+ ions, and we investigate in detail the effects of environmental conditions such as electrolyte solution, pH, and the concentration range on the performance of the device. Then, we use the larger peptide OT, which is selective for Cu2+ and Zn2+ ions, for the detection and differentiation of the binding of these two ions in a solution. Finally, we combine atomic force microscopy (AFM), Raman spectroscopy, and molecular dynamic (MD) simulations to characterize the aggregation pathway of alpha-synuclein (αSyn), a protein that plays a key role in the pathogenesis of Parkinson's disease. We found that the presence of Cu2+ ions facilitates the aggregation process. Our measurements were conducted with the purest form of protein in buffer conditions, which can help to understand the aggregation processes happening in the brain in the future by correlating the results with clinical data from patients, obtained from cerebrospinal fluid (CSF) or blood

Detection of Cu2+ Ions with GGH Peptide Realized with Si-Nanoribbon ISFET

The presence of heavy metal ions such as copper in the human body at certain concentrations and specific conditions can lead to the development of different diseases. The currently available analytical detection methods remain expensive, time-consuming, and often require sample pre-treatment. The development of specific and quantitative, easy-in-operation, and cost-effective devices, capable of monitoring the level of Cu2+ ions in environmental and physiological media, is necessary. We use silicon nanoribbon (SiNR) ion-sensitive field effect transistor (ISFET) devices modified with a Gly–Gly–His peptide for the detection of copper ions in a large concentration range. The specific binding of copper ions causes a conformational change of the ligand, and a deprotonation of secondary amine groups. By performing differential measurements, we gain a deeper insight into the details of the ion–ligand interaction. We highlight in particular the importance of considering non-specific interactions to explain the sensors’ response

3D Holo-tomographic Mapping of COVID-19 Microclots in Blood to Assess Disease Severity

The coronavirus disease 2019 (COVID-19) has impacted health globally. Cumulative evidence points to long-term effects of COVID-19 such as cardiovascular and cognitive disorders, diagnosed in patients even after the recovery period. In particular, micrometer-sized blood clots and hyperactivated platelets have been identified as potential indicators of long COVID. Here, we resolve microclot structures in the plasma of patients with different subphenotypes of COVID-19 in a label-free manner, using 3D digital holo-tomographic microscopy (DHTM). Based on 3D refractive index (RI) tomograms, the size, dry mass, and prevalence of microclot composites were quantified and then parametrically differentiated from fibrin-rich microclots and platelet aggregates in the plasma of COVID-19 patients. Importantly, fewer microclots and platelet aggregates were detected in the plasma of healthy controls compared to COVID-19 patients. Our imaging and analysis workflow is built around a commercially available DHT microscope capable of operation in clinical settings with a 2 h time period from sample preparation and data acquisition to results

Single-Particle Resolution of Copper-Associated Annular α‑Synuclein Oligomers Reveals Potential Therapeutic Targets of Neurodegeneration

 Metal ions stabilize protein−protein interactions and can modulate protein aggregation. Here, using liquid-based atomic force microscopy and molecular dynamics simulations, we study the concentration-dependent effect of Cu2+ ions on the aggregation pathway of α-synuclein (α-Syn) proteins, which play a key role in the pathology of Parkinson’s disease. The full spectrum of α-Syn aggregates in the presence and absence of Cu2+ ions from monomers to mature fibrils was resolved and quantified at the gold−water interface. Raman spectroscopy confirmed the atomic force microscopy (AFM) findings on the heterogeneity in aggregated states of α-Syn. The formation of annular oligomers was exclusively detected upon incubating α-Syn with Cu2+ ions. Our findings emphasize the importance of targeting annular α-Syn protein oligomers for therapeutic intervention and their potential role as biomarkers for early detection and monitoring progression of neurodegeneration. </p