FRENECTOMY ON MAXILLARY LABIAL FRENULUM PENETRATING PAPILLA TYPE BY CONVENTIONAL SURGICAL TECHNIQUES: A CASE REPORT

Introduction: The frenulum is a fold of mucosa that attaches the lips and cheeks to the alveolar mucosa, gingiva, and underlying periosteum. If the attachment is too high and thick to approach the gingival margin (papilla penetrating type) it causes a central diastema on the upper incisor or recurrence after orthodontic treatment, besides causing oral hygiene problems and gingival recession as well as denture stability. An abnormal frenulum should be removed by frenectomy. History and Clinical Finding: An 18-year-old female patient with a tall and thick maxillary labial frenulum as a cause of central diastema on teeth 11, 21. The patient had been treated with removable orthodontics starting 2 years ago. Since 4-6 months ago the diastema has relapse. The maxillary labial frenulum appears to be attached to the incisive papilla (penetration of the papillae). The Blanch test shows movement of the interdental papillae and blanching (ischemia) of the incisor papillae. Case Management: Frenectomy using a scalpel until all the papillae and fibrous fibers are removed. Control 14 days postoperative, the wound was healed. During the blanching test, there was no movement of the papillae and the color was normally. Discussions: The high maxillary labial frenulum causes the two central incisors to erupt far apart. Orthodontic treatment  must be accompanied by removal of the etiological factor by frenectomy for the treatment to be successful. Conclusions: Penetrating papilla type frenectomy of the maxillary labial frenulum with the conventional technique of using a scalpel effectively eliminates the causes of central diastema

INHIBITORY POWER OF MAS BANANA PEEL EXTRACT (MUSA ACUMINATA LADY FINGER) ON THE GROWTH OF STAPHYLOCOCCUS AUREUS IN VITRO

Introduction: Various sources of infectious disease can be found in the oral cavity. Prevention efforts include brushing your teeth properly and regularly. Besides that, gargling with mouthwash is one of the best additional prevention methods. In the literature it is known that mas banana peels contain antibacterial ingredients. The aim of this research was to determine whether banana peel extract (Musa acuminata lady finger) was able to inhibit the growth of Staphylococcus aureus bacteria.  Material and Methods: Extracts of 25%, 50%, 75% concentration of dried mas banana peel were made, sterile distilled water as a negative control and 0.2% Chlorhexidine as a positive control. Staphylococcus aureus ATCC 25923 as many as 3 bacterial colonies, spread evenly on sterile Nutrient Agar (NA) media. The paper discs were dipped for ± 30 seconds into 25%, 50%, 75% gold banana peel extract, 0.2% Chlorhexidine as a positive control and sterile Aquades as a negative control then incubated in an incubator at 37°C for 24 hours. The inhibition zone (clear area around the paper disc) was measured with a caliper (accuracy 0.02mm). The data obtained were tested for normality and homogeneity to determine whether or not there was a difference in the inhibitory power of banana mas (Musa acuminata lady finger) peel extract against Staphylococcus aureus bacteria. Results and Discussion: The results showed that at a concentration of 75% there was an inhibitory power for the growth of Staphylococcus aureus bacteria of 6.32 mm. Mas banana peel extract (Musa acuminata lady finger) has antibacterial content in the form of flavonoids, alkaloids, saponins and tannins which are able to inhibit the growth of Staphylococcus aureus bacteria in vitro. Conclusion: Mas banana peel extract (Musa acuminata lady finger) can inhibit the growth of Staphylococcus aureus bacteria in vitro with moderate criteria

Vitamin D inhibits TNF-α serum level in wistar rats stimulated with Porphyromonas gingivalis

Periodontitis is a chronic inflammation of the periodontal tissues initiated by increased proinflammatory cytokines such as TNF-α due to the induction of Porphyromonas gingivalis (Pg). This study aimed to analyze the effect of vitamin D (cholecalciferol) on TNF-α serum level in Wistar rats stimulated with Pg to induce periodontitis. Twenty-seven male Wistar rats (n = 27) were divided into three equal groups. Group I was healthy Wistar rats that received 2000 IU vitamin D once a day. Group II was Wistar rats stimulated with Pg and received 2000 IU vitamin D, while group III was Wistar rats stimulated with Pg but did not receive vitamin D. Blood was collected through the orbital sinus and centrifuged to get the serum. TNF-α serum levels were assessed using Elisa method on the 7th, 14th, and 28th. The data were normally distributed and homogeneous. The mean TNF-α data was analyzed for differences between groups using the one-way ANOVA and LSD post hoc test. Significant differences were seen in group II (418.49 ± 161.08 ng/mL) and group III (172.16 ± 104.18 ng/mL) on the 28th day (p = 0.001). The findings suggest that vitamin D inhibits the TNF-α serum level in Wistar rats stimulated with Pg on the 28th day

OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO : Konsentrasi Optimal Dari Ekstrak Daun Kemangi (Ocimum Basilicum L.) Dalam Menghambat Pertumbuhan Bakteri Streptococcus Mutans In Vitro

Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro

THE EFFECTIVENESS OF PROBIOTIC LOZENGES LACTOBACILLUS REUTERI PRODENTIS IN INCREASING SALIVARY SECRETION : Efektivitas Tablet Hisap Probiotik Lactobacillus Reuteri Prodentis Terhadap Sekresi Saliva

Introduction: Indonesian people experience dental and oral health problemsas much as 57.6%, one of the dental and oral health problems that is oftentreated is periodontal disease. Alternative prevention of periodontal disease isby consuming probiotics (Lactobacillus reuteri). Probiotic (Lactobacillusreuteri) contains L. reuteri bacteria which inhibits periodontal and cariogenicpathogenic bacteria so that it is suspected to increase salivary flow rate. Objectives: to determine the effectiveness of probiotics (Lactobacillus reuteri) to increase salivary secretion. Materials and methods: this studyused an experimental approach with a pre-test and post-test design. The research subjects were 30 students of the Faculty of Dentistry, MahasaraswatiDenpasar University who met the research criteria. The sample was dividedinto two groups, namely the group that consumed the probiotic Interlac Pro-D lozenges and the group that consumed a placebo. The data taken werethe salivary flow rate, which was measured twice, before and after administration of the probiotic Interlac Pro-D lozenges and placebo. Dataanalysis: data is processed using paired t-test and independent t-test. Resultsand Discussion: The results showed that the average salivary secretion before consuming Interlac Pro-D probiotic tablets was 0.3073 ± 0.02492 ml/minute, increasing to 0.328 ± 0.03299 ml/minute, placebo increasing to 0.276± 0.02293 ml/minute to 0.2853 ± 0.0256 ml/minute. There was a differencein the effectiveness of increasing salivary secretion between the groups consuming the probiotic Interlac Pro-D tablets and placebo (p <0.005). Conclusion: taking probiotic lozenges Interlac Pro-D (Lactobacillus reuteriprodentis) is effective in increasing salivary secretion which is beneficial forpreventive therapy of periodontal disease

PLAQUE CONTROL IN PERIODONTAL DISEASE: KONTROL PLAK PADA PENYAKIT PERIODONTAL

Introduction: Periodontal disease results from a complex interplay between the subgingival biofilm and the host immune-immflamatory events that develop in the gingival and periodontal in response to the challenge presented by the bacteria. Gingivitis precedes periodontitis. In gingivitis , the inflammatory lesion is confined to the gingiva, however, with periodontits, the inflammatory processes extend to additionally affect the periodontal ligament and the alveolar bone. Review: The primary cause of gingival inflammattion is bacterial plaque. Other predisposing factors include calculus, faulty restoration complication associated with orthodontic therapy. Microbial plaque biofilm control is an effective way of treating and preventing gingivitis and is an essential part of all procedures involved in the treatment and prevention of periodontal diseases. Good supragingival biofilm control has also been shown to affect the growth and composition of subgingival plaque biofilm so that it favors a healthier microflora and reduces calculus formation. Carefully performed daily home plaque biofilm control, combined with frequent professionally delivered plaque biofilm and calculus removal, reduces the amount of supragingival biofilm, decreases the total number of microorganisms in moderately deep pockets, including furcation areas, and greatly reduces the quantity of periodontal pathogens. Chemical inhibitors of plaque biofilm and calculus that are incoporated in mouthwashes or dentifrices also play an important role in controlling microbial biofilms. Conclusion: Daily plaque biofilm control permits patients to assume responsibility for their own oral health every day