Work in Progress -- Tailoring Optics Resources for K-5 Pre-college Outreach

Pre-college instruction in science, technology, Engineering; and mathematics (STEM) is critical to preparing a workforce and citizens for a technology-based society. The K-5 years are especially important to creating interest and instilling basic concepts. Engineering-based pre-college outreach can provide useful resources in the K- 5 education classroom. However, interaction among K-5 teachers and STEM professionals is more effective if outreach is tailored to the K-5 environment. In particular, resources and outreach should accommodate K-5 assessment standards, level-appropriate concept definition, and grade continuity. This work uses a set of optics resources as an example of tailored outreach. Example lessons and activities illustrate pedagogy appropriate for the unique K-5 education environment, e.g. auditory, visual, and kinesthetic components, concept definition, terminology, and grade continuity. Optical engineering topics are related to Missouri science standards, STEM relevance and inquiry, and measurement activities

Work-in-Progress: Statistics Components in a Circuits Laboratory for ECE Sophomores

Statistics concepts are required for undergraduate curricula in electrical engineering and computer engineering (ECE). Accreditation guidelines specify that such instruction must include “applications appropriate to the program name” [ABET Criteria for Accrediting Engineering Programs, 2014-2015]. Curricula content in probability and statistics are commonly done in a stand-alone course, but additional exposure within engineering-content courses can provide great value. Many basic concepts can be introduced before the comprehensive stand-alone course. A good ECE context is an electrical circuits laboratory in which students need to understand electrical component tolerances and related design issues. Statistics-related assignments in a required, sophomore-level laboratory course are described for applications involving resistor tolerances. Exposure to basic statistics concepts and computer resources in an introductory in-major course facilitates more advanced instruction in follow-on in-major courses

RFID Instrumentation in a Field Application

The behavior of radio-frequency identification (RFID) tags is investigated in a field application. The RFID instrumentation is used to mark the position of point strain sensors in a fiber-reinforced polymer (FRP), short-span bridge. The tags are surface mounted on carbon FRP structural elements and covered with a resin wear layer, i.e. the deck of the bridge. The smart composite bridge was installed in 2000 and serves as a field testbed for various sensor technologies including the RFID tags. The operation of the RFID network is tested in the field and compared to laboratory tags. Specific research issues include long-term survivability after six years, behavior on carbon FRP material, and environmental effects of weather, especially the presence of water. The feasibility of short-range RFID tags as location markers is discussed. The results show the suitability of RFID technology for field environments

Involving Engineering with In-Service K-4 Teachers

A strong workforce in science and engineering and literate citizens in a technology-based society depends on an educational system that prepares students in science, mathematics, and engineering. Unfortunately, many K-12 students lose interest in such topics early in their school years. Engineering applications can capture the imagination of students and illustrate the concepts in science and mathematics. Level-appropriate resources are as necessary for primary teachers and our young scholars as for teachers and students in higher grades. A model for interaction between engineers and K-4 teachers is proposed. The State of Missouri defines curriculum standards in each subject area and assesses student performance three times during a student\u27s education. The first assessment for science is in the third grade and for mathematics is in the fourth grade. These standards were examined from an engineering perspective. In addition, in-service primary teachers were surveyed to determine their background, needs, and attitudes regarding science, mathematics, and engineering. Early years of an effective technical curriculum must introduce the basic concepts and begin layering this understanding with detail and connectivity. Our model shows how engineering applications can provide hearing, seeing, and doing components, i.e. auditory, visual, and kinesthetic educational activities, within the recommended pedagogy. The approach involves in-service teachers throughout the development process, addresses state standards and testing criteria, and partners specialists in engineering content and teacher training

Work in Progress -- Instrumentation on a Truss Adapted for Pre-College Outreach

Engineering content is a valuable addition to pre-college instruction in science, technology, engineering, and mathematics (STEM) since it applies scientific concepts, illustrates scientific relevance and technology, and provides measurement opportunities. Also, complex systems and interactions can be shown. This work describes outreach resources using a seven-member instrumented truss apparatus. This aluminum bench-top model is scaled to support up to fifty pounds. Electrical resistance gauges are installed on several members for strain measurement. The resource set includes the truss apparatus, instrumentation, a PowerPoint presentation, and a background document. The pre-college objective is a set of demonstration resources for middle or high school classrooms. Effective outreach design is modeled by tailoring to accommodate curriculum standards, level-appropriate concept terms, and grade continuity. The resources were developed by students in an interdisciplinary college class on sensors and structures. The development activities involved testing the models and measurements and refining the construction. Selected resources were implemented and evaluated in a local middle school classroom. The interdisciplinary content includes structural, force analysis, sensing, and measurement components

Vimentin DNA methylation predicts survival in breast cancer

The Vimentin gene plays a pivotal role in epithelial-to-mesenchymal transition and is known to be overexpressed in the prognostically poor basal-like breast cancer subtype. Recent studies have reported Vimentin DNA methylation in association with poor clinical outcomes in other solid tumors, but not in breast cancer. We therefore quantified Vimentin DNA methylation using MALDI-TOF mass spectrometry in breast tumors and matched normal pairs in association with gene expression and survival in a hospital-based study of breast cancer patients. Gene expression data via qRT-PCR in cell lines and oligomicroarray data from breast tissues were correlated with percent methylation in the Vimentin promoter. A threshold of 20 percent average methylation compared with matched normal pairs was set for bivariate and multivariate tests of association between methylation and tumor subtype, tumor histopathology, and survival. Vimentin was differentially methylated in luminal breast cancer cell lines, and in luminal A, luminal B, and HER2-enriched breast tumor subtypes, but was rare in basal-like cell lines and tumors. Increased methylation was strongly correlated with decreased mRNA expression in cell lines, and had a moderate inverse correlation in breast tumors. Vimentin methylation predicted poor overall survival independent of race, subtype, stage, nodal status, or metastatic disease and holds promise as a new prognostic biomarker for breast cancer patients

Differential methylation relative to breast cancer subtype and matched normal tissue reveals distinct patterns

Due to the heterogeneous nature of breast cancer and the widespread use of single-gene studies, there is limited knowledge of multi-gene, locus-specific DNA methylation patterns in relation to molecular subtype and clinical features. We, therefore, quantified DNA methylation of 70 candidate gene loci in 140 breast tumors and matched normal tissues and determined associations with gene expression and tumor subtype. Using Sequenom’s EpiTYPER platform, approximately 1,200CpGs were interrogated and revealed six DNA methylation patterns in breast tumors relative to matched normal tissue. Differential methylation of several gene loci was observed within all molecular subtypes, while other patterns were subtype-dependent. Methylation of numerous gene loci was inversely correlated with gene expression, and in some cases, this correlation was only observed within specific breast tumor subtypes. Our findings were validated on a larger set of tumors and matched adjacent normal tissue from The Cancer Genome Atlas dataset, which utilized methylation data derived from both Illumina Infinium 27 and 450k arrays. These findings highlight the need to control for subtype when interpreting DNA methylation results, and the importance of interrogating multiple CpGs across varied gene regions.Electronic supplementary materialThe online version of this article (doi:10.1007/s10549-013-2738-0) contains supplementary material, which is available to authorized users

Human Cytomegalovirus IE1 Protein Elicits a Type II Interferon-Like Host Cell Response That Depends on Activated STAT1 but Not Interferon-γ

Human cytomegalovirus (hCMV) is a highly prevalent pathogen that, upon primary infection, establishes life-long persistence in all infected individuals. Acute hCMV infections cause a variety of diseases in humans with developmental or acquired immune deficits. In addition, persistent hCMV infection may contribute to various chronic disease conditions even in immunologically normal people. The pathogenesis of hCMV disease has been frequently linked to inflammatory host immune responses triggered by virus-infected cells. Moreover, hCMV infection activates numerous host genes many of which encode pro-inflammatory proteins. However, little is known about the relative contributions of individual viral gene products to these changes in cellular transcription. We systematically analyzed the effects of the hCMV 72-kDa immediate-early 1 (IE1) protein, a major transcriptional activator and antagonist of type I interferon (IFN) signaling, on the human transcriptome. Following expression under conditions closely mimicking the situation during productive infection, IE1 elicits a global type II IFN-like host cell response. This response is dominated by the selective up-regulation of immune stimulatory genes normally controlled by IFN-γ and includes the synthesis and secretion of pro-inflammatory chemokines. IE1-mediated induction of IFN-stimulated genes strictly depends on tyrosine-phosphorylated signal transducer and activator of transcription 1 (STAT1) and correlates with the nuclear accumulation and sequence-specific binding of STAT1 to IFN-γ-responsive promoters. However, neither synthesis nor secretion of IFN-γ or other IFNs seems to be required for the IE1-dependent effects on cellular gene expression. Our results demonstrate that a single hCMV protein can trigger a pro-inflammatory host transcriptional response via an unexpected STAT1-dependent but IFN-independent mechanism and identify IE1 as a candidate determinant of hCMV pathogenicity