10 research outputs found
Extension of StructureâReactivity Correlations for the Hydrogen Abstraction Reaction to the Methyl Radical and Comparison to the Chlorine Atom, Bromine Atom, and Hydroxyl Radical
No full textMultiplex transcriptional characterizations across diverse bacterial species using cellâfree systems
No full textAbstract Cellâfree expression systems enable rapid prototyping of genetic programs in vitro. However, current throughput of cellâfree measurements is limited by the use of channelâlimited fluorescent readouts. Here, we describe DNA Regulatory element Analysis by cellâFree Transcription and Sequencing (DRAFTS), a rapid and robust in vitro approach for multiplexed measurement of transcriptional activities from thousands of regulatory sequences in a single reaction. We employ this method in active cell lysates developed from ten diverse bacterial species. Interspecies analysis of transcriptional profiles from > 1,000 diverse regulatory sequences reveals functional differences in promoter activity that can be quantitatively modeled, providing a rich resource for tuning gene expression in diverse bacterial species. Finally, we examine the transcriptional capacities of dualâspecies hybrid lysates that can simultaneously harness gene expression properties of multiple organisms. We expect that this cellâfree multiplex transcriptional measurement approach will improve genetic part prototyping in new bacterial chassis for synthetic biology
Multiplex transcriptional characterizations across diverse bacterial species using cellâfree systems
No full textAbstract Cellâfree expression systems enable rapid prototyping of genetic programs in vitro. However, current throughput of cellâfree measurements is limited by the use of channelâlimited fluorescent readouts. Here, we describe DNA Regulatory element Analysis by cellâFree Transcription and Sequencing (DRAFTS), a rapid and robust in vitro approach for multiplexed measurement of transcriptional activities from thousands of regulatory sequences in a single reaction. We employ this method in active cell lysates developed from ten diverse bacterial species. Interspecies analysis of transcriptional profiles from > 1,000 diverse regulatory sequences reveals functional differences in promoter activity that can be quantitatively modeled, providing a rich resource for tuning gene expression in diverse bacterial species. Finally, we examine the transcriptional capacities of dualâspecies hybrid lysates that can simultaneously harness gene expression properties of multiple organisms. We expect that this cellâfree multiplex transcriptional measurement approach will improve genetic part prototyping in new bacterial chassis for synthetic biology
Interaction between Ionic and Nonionic Surfactants in the Adsorbed Film and Micelle: Hydrochloric Acid, Sodium Chloride, and Tetraethylene Glycol Monooctyl Ether
No full textHidden in plain sight: mixed methods and the marble orchards of the Vlach Rom (Gypsies) in Toledo, Ohio
No full textâGypsies to the Camps!â: Exclusion and Marginalisation of Roma in the Czech Republic
No full text
