DETERMINATION OF MICROSCOPIC OBJECTS DIMENSIONS USING DIGITAL IMAGES

The proposed method is less time consuming, since the microscope is needed only for obtaining the image of the measured object, as established of the object-micrometer or images of the Goryaev camera grid. Working with a scale ruler and an image is more comfortable and less difficult than working with a microscope, an ocular micrometer and a coordinate microscope table. Working with an image, unlike working with a microscope, is convenient for people using glasses as it reduces the burden on the researcher’s vision.</p

СРАВНИТЕЛЬНАЯ АНТИФУНГАЛЬНАЯ АКТИВНОСТЬ ИЗОЛЯТОВ BACILLUS SUBTILIS И BACILLUS THURINGIENSIS IN VITRO

ACTIVITATEA COMPARATIVĂ ANTIFUNGICĂ A IZOLATELOR DE BACILLUS SUBTILIS ŞI BACILLUS THURINGIENSIS IN VITROSunt arătate rezultatele evaluării comparative a acţiunii antifungice a trei culturi de B. subtilis şi a reprezentanţilor a trei subspecii de B. thuringiensis împotriva ciupercilor fitopatogene Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum var. orthoceras şi Alternaria alternata în condiţiile experimentului în vitro. Subspeciile de B. thuringiensis au activitate antifungică mai slabă decât B. subtilis. Cel mai activ s-a dovedit a fi B. thuringiensis var. kurstaki.COMPARATIVE ANTIFUNGAL ACTIVITY OF BACILLUS SUBTILIS AND BACILLUS THURINGIENSIS ISOLATES IN VITROThe results of antifungal activity comparative assessment of three B. subtilis culture and representatives of three B. thuringiensis subspecies against phytopathogenic fungi Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, Fusarium oxysporum var. orthoceras and Alternaria alternata in the in vitro experience are presented. B. turingiensis subspecies antifungal activity was less than B. subtilis one. B. thuringiensis var. kurstaki was the most active among B. thuringiensis subspecies.</p

СПОСОБ ОТСРОЧЕННОГО УЧЕТА ЧИСЛА КОЛОНИЙ НА ЧАШКАХ ПЕТРИ

METODĂ DE EVIDENŢĂ AMÂNATĂ A NUMĂRULUI COLONIILOR DE MICROORGANISME ÎN CUTII PETRISe propune o metodă de evidenţă a coloniilor de microorganisme în cutii Petri prin analiza imaginilor digitale, marcarea coloniilor evidenţiate într-un program pentru crearea şi editarea desenelor Microsoft Paint şi înregistrarea numărului lor într-o fereastră Microsoft Office Excel deschisă paralel. Obţinerea imaginilor digitale permite a documenta, arhiva şi evidenţia datele în orice moment potrivit. Posedând toate avantajele aparatului pentru numărarea coloniilor în cutii Petri, metoda propusă de noi evită necesitatea de echipament special şi permite a evidenţia coloniile oricărui microorganism.THE METHOD OF DEFERRED ACCOUNTING OF COLONIES NUMBER IN PETRI DISHESA method for colony account in Petri dishes by analyzing digital images of dishes and marking of recorded colonies by means of computer graphics editor Microsoft Paint and a registration of recorded colonies number in the new open Microsoft Office Excel window is proposed. Obtaining digital images allows documentation and archivation the data and to carry out account at any desired time. Possessing all device advantages for counting colonies in Petri dishes, the method allows to avoid any other special devices for this purpose and to account any kinds of microorganism colonies.</p

Case of Meningitis in a Neonate Caused by an Extended-Spectrum-Beta-Lactamase-Producing Strain of Hypervirulent Klebsiella pneumoniae

Klebsiella pneumoniae is one of the most important infectious agents among neonates. This pathogen has a potential to develop an increased antimicrobial resistance and virulence. The classic non-virulent strain of K. pneumoniae, producing an extended-spectrum beta-lactamases (ESBL), is associated with nosocomial infection mainly in preterm neonates. Hypervirulent K. pneumoniae strains are associated with invasive infection among previously healthy ambulatory patients, and most of them exhibit antimicrobial susceptibility. During the last few years, several cases of diseases caused by hypervirulent K. pneumoniae producing ESBL have been registered in different geographical regions of the world. However, reports of such cases in neonates are rare. Here, we reported that this pathogen can cause pyogenic meningitis in full-term neonate with poor prognosis. A previously healthy, full-term, 12-day-old neonate was admitted to the infectious diseases hospital with suspected meningitis. The clinical symptoms included loss of appetite, irritability, fever, seizures, and a bulging anterior fontanelle. The analysis of the cerebrospinal fluid confirmed the diagnosis of meningitis. Blood and cerebrospinal fluid cultures were positive for K. pneumoniae, producing ESBL. K. pneumoniae isolates were resistant to aminopenicillins, 3rd generation cephalosporins but were sensitive to imipenem and meropenem. The “string test” was positive. The study of the virulence factors of K. pneumoniae by PCR revealed the presence of the rmpA gene. A combination of K. pneumoniae virulence and drug resistance complicated by cerebral oedema led to the death of the neonate. We concluded that both the risk of developing severe forms of infection and the outcome of the disease due to K. pneumonia are associated with the phenotypic features of the pathogen such as its antibiotic susceptibility and virulence factors. Emergence of the ESBL-producing strain of hypervirulent K. pneumoniae could represent a new serious threat to public health, suggesting an urgent need to enhance clinical awareness and epidemiological surveillance