3 research outputs found
Dose-Dependent Effects of Nanoscale Forms of Humic Acids in a Hydroponic Culture of Triticum aestivum: Induction of Oxidative Stress and an Increase in the Number of Border Cells
Π Π³ΠΈΠ΄ΡΠΎΠΏΠΎΠ½Π½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠ΅ Triticum aestivum L. ΠΈΠ·ΡΡΠ°Π»ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ
Π³ΡΠΌΠΈΠ½ΠΎΠ²ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ Π² ΡΠΎΡΠΌΠ΅ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΡΠ°Π·ΠΌΠ΅ΡΠΎΠΌ 6,5 Π½ΠΌ ΠΈ 68 Π½ΠΌ. ΠΡΠΌΠΈΠ½ΠΎΠ²ΡΠ΅ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ (ΠΌΠ΅Π»ΠΊΠΈΠ΅
ΠΈ ΠΊΡΡΠΏΠ½ΡΠ΅) Π² ΠΈΠ·ΡΡΠ΅Π½Π½ΡΡ
ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡΡ
Π½Π΅ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π»ΠΈ ΡΠΎΡΡ ΠΊΠΎΡΠ½Ρ ΠΈ Π½Π΅ Π²ΡΠ·ΡΠ²Π°Π»ΠΈ Π΄Π΅ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΠΊΠΎΡΠ½Π΅Π²ΠΎΠ³ΠΎ Π°ΠΏΠ΅ΠΊΡΠ° 2-Π΄Π½Π΅Π²Π½ΡΡ
ΠΏΡΠΎΡΠΎΡΡΠΊΠΎΠ². ΠΠ° ΡΡΠ΅Π΄Π°Ρ
Ρ Π³ΡΠΌΠΈΠ½ΠΎΠ²ΡΠΌΠΈ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΠ°ΠΌΠΈ
Π² ΠΊΠΎΡΠ½ΡΡ
ΠΏΡΠΎΡΠΎΡΡΠΊΠΎΠ² Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΎΡΡ Π΄ΠΎΠ·ΠΎΠ·Π°Π²ΠΈΡΠΈΠΌΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΊΠ°ΡΠ±ΠΎΠ½ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π±Π΅Π»ΠΊΠΎΠ², ΠΌΠ°Π»ΠΎΠ½ΠΎΠ²ΠΎΠ³ΠΎ Π°Π»ΡΠ΄Π΅Π³ΠΈΠ΄Π° ΠΈ ΠΏΡΠΎΠ»ΠΈΠ½Π° β ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΡΡΠ°.
ΠΠ»Ρ ΠΌΠ΅Π»ΠΊΠΈΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΠΌ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠΌ ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΠΎΡΠ°Π΄ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠΊΠΈΡΠ»Π΅Π½ΠΈΡ Π±ΡΠ» ΠΌΠ°Π»ΠΎΠ½ΠΎΠ²ΡΠΉ
Π΄ΠΈΠ°Π»ΡΠ΄Π΅Π³ΠΈΠ΄, Π΄Π»Ρ ΠΊΡΡΠΏΠ½ΡΡ
β ΠΊΠ°ΡΠ±ΠΎΠ½ΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ. ΠΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ Π³ΡΠΌΠΈΠ½ΠΎΠ²ΡΠΌΠΈ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΠ°ΠΌΠΈ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΡΡΠ΅ΡΡ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π»ΡΡ Π΄ΠΎΠ·ΠΎΠ·Π°Π²ΠΈΡΠΈΠΌΡΠΌ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠΈΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ
ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΡΡ
ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΡΠ°Π·ΠΌΠ΅ΡΠΎΠ² Π³Π΅Π»Π΅Π²ΠΎΠ³ΠΎ ΡΠ΅Ρ
Π»Π° Π² ΠΊΠΎΡΠ½Π΅Π²ΠΎΠΌ Π°ΠΏΠ΅ΠΊΡΠ΅ 2-Π΄Π½Π΅Π²Π½ΡΡ
ΠΏΡΠΎΡΠΎΡΡΠΊΠΎΠ². ΠΠ»Ρ ΠΌΠ΅Π»ΠΊΠΈΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ (6,5 Π½ΠΌ) ΡΡΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π±ΡΠ»ΠΈ Π²ΡΡΠ°ΠΆΠ΅Π½Ρ Π² Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ
Π±ΠΎΠ»ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ, ΡΠ΅ΠΌ Π΄Π»Ρ ΠΊΡΡΠΏΠ½ΡΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ (68 Π½ΠΌ). ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ
ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ Π³ΡΠΌΠΈΠ½ΠΎΠ²ΡΠ΅ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π°Π΄Π°ΠΏΡΠΎΠ³Π΅Π½ΠΎΠ²: ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ
ΡΠΈΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΡΠΈΡΡΠ΅ΠΌΡ Π³Π΅Π»Π΅Π²ΠΎΠ³ΠΎ ΡΠ΅Ρ
Π»Π° (ΠΊΠ°ΠΊ ΠΏΡΠΎΠ΄ΡΠΊΡΠ° ΡΠΊΡΠΊΡΠ΅ΡΠΎΡΠ½ΠΎΠΉ
Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ), Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°ΡΡ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡ ΠΊΠΎΡΠ½Π΅Π²ΠΎΠ³ΠΎ Π°ΠΏΠ΅ΠΊΡΠ°
ΠΊ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π½Π΅Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½ΡΡ
Π±ΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ Π°Π±ΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ²The biological activity of humic acids in the form of nanoparticles with a size of 6.5 nm and 68 nm was studied in a hydroponic culture of Triticum aestivum L. Both smaller and larger humic nanoparticles at the studied concentrations neither inhibited root growth nor caused destructive changes in the root apexes of 2-day-old seedlings. A dose-dependent increase in the contents of markers of induced oxidative stress such as carbonylated proteins, malonaldehyde, and proline was observed in the roots of seedlings on media with humic nanoparticles. Malondialdehyde was the main product of free radical oxidation for smaller nanoparticles and carbonylated proteins β for larger ones. Oxidative stress induced by humic nanoparticles was accompanied by a dose-dependent increase in the number of free border cells and the size of the mucilaginous sheaths on the root apexes of 2-day-old seedlings. For smaller (6.5 nm) nanoparticles, these changes were much more pronounced than for larger (68 nm) nanoparticles. The results obtained allow us to consider humic nanoparticles as adaptogens: contributing to an increase in the population of border cells and the mucilaginous sheath system (as a product of the excretory activity of border cells), nanoparticles enhance the resistance of the root apex to the effects of unfavorable biotic and abiotic factor
The Role of Periodic Structures in Light Harvesting
The features of light propagation in plant leaves depend on the long-period ordering in chloroplasts and the spectral characteristics of pigments. This work demonstrates a method of determining the hidden ordered structure. Transmission spectra have been determined using transfer matrix method. A band gap was found in the visible spectral range. The effective refractive index and dispersion in the absorption spectrum area of chlorophyll were taken into account to show that the density of photon states increases, while the spectrum shifts towards the wavelength range of effective photosynthesis
The Morphology and Phenotype of Monocyte-Macrophages When Cultured on Bionanofilms Substrates with Different Surface Relief Profiles
The effect of surface relief profiles of alkanoate-based bionanofilms to the monocyte-macrophages (MN-MPhs) from peripheral blood of patients with atherosclerosis was studied in vitro. Patients were subjected to coronary stenting. Cell morphology and phenotype (expression of CD antigens, levels of production of marker cytokines) in vitro were analyzed before and after the installation of stents. It was shown, that the mean square roughness (Rq) of the bionanofilms determined the variability of cell morphology, CD antigens spectraand activity of production interleukins-6 and -10. Also, it was revealed, that the “activity” of the surface topography of biopolymer substrates depends on the functional state of MNs, isolated in different time points: Before and after stenting the ratios of cell morphotypes and production of cytokines in MN-MPhs differed significantly