Development and Applications of Fluorinated Oligomeric Nanocomposites Imparted by Ionic Liquids

弘前大学博士（工学）doctoral thesi

Application of Hansch’s Model to Capsaicinoids and Capsinoids: A Study Using the Quantitative Structure−Activity Relationship. A Novel Method for the Synthesis of Capsinoids

We describe a synthetic approach for two families of compounds, the capsaicinoids and capsinoids, as part of a study of the quantitative relationship between structure and activity

Nafamostat Mesilate（ FUT-175） Inhibits Cell Growth and Invasion of Malignant Pleural Mesothelioma Cell Line, MSTO-211H

Nafamostat mesilate（ FUT-175）, a synthetic serine protease inhibitor, has been reported to have antitumouractivities toward solid tumours. The objective of this study was to characterize the biological activities of FUT-175 ina malignant mesothelioma cell line. We used MSTO-211H, a biphasic-type human malignant pleural mesothelioma cellline. The effect on cell growth was evaluated by 3（- 4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay.Secretion of urokinase-type plasminogen activator（ u-PA） and plasminogen activator inhibitor-1（ PAI-1） was analysedby enzyme-linked immunosorbent assay. The eff ects on relative mRNA expression levels were measured by reversetranscription polymerase chain reaction. The eff ect on cell invasiveness was evaluated by cell invasion assay. FUT-175at 10-5 M signifi cantly inhibited cell growth and cell invasiveness. Cell growth reduced to 47.0 ± 2.1% compared withthe control. The number of invasive cells also reduced to 16.0 ± 0.7 cells/hpf, while that of control cells was 41.4 ± 8.0cells /hpf. U-PA and PAI-1 secreted from the cells were also reduced by FUT-175 in a dose-dependent manner. Theseresults suggest that FUT-175 has the potential to act as a therapeutic agent against local growth and invasion, andfunctions by reducing PAI-1 and u-PA production of the human malignant mesothelioma（195 words）弘前医学. 61(1), 2010, p.19-25journal articl

Studies on Silage-Making : On the Effect of Some Additives

The author has already published a few reports on the silage-making with some additives. In this paper the result of experiments carried on with 'Shochukasu' (lees of a distilled spirit made from sweet potatoes), starch feed (a by-product in starch manufacture), urea and A.I.V. acid will be reported. (1) A 7 : 3 mixture of white clover and wild plants was respectively preserved either with 'Shochukasu' or with starch feed as additives (cf. Table 1). Investigations on organic acids, the pH value, and the chemical composition of the silages, palatability to live-stock and on nutrient losses during the storage were made, and it was recognized that 'Shochukasu' and starch feed were effective as the additives to the silage-making. The ensiling may be expected as an effective preservation method of 'Shochukasu' itself. The silage prepared with 'Shochukasu' added at the rate of 43 per cent of the green plant seemed to be the most palatable feed to various amimals (cf. Table 4). (2) The use of the amount of 1 per cent of urea in silage-making with the sweet potato and rice bran (80 : 20) seemed to be harmless and the resulting silage was palatable to ruminants ; it had the following properties : pH 4.3, free lactic acid content 2.02, acetic acid content 0.53 and butyric acid content 0 per cent. And sweet potato vine silage prepared with the amount of 1 per cent of urea did not contain butyric acid either. (3) The teosinte silage prepared according to A.I.V. method had very good quality : pH 3.5, lactic acid content 1.58, acetic acid content 0.29 and butyric acid content 0 per cent. The nutrient losses in fermentation process were only 4 per cent in dry matter and 5 per cent in organic matter

Studies on the factors determining the quality of silage.

Analyzing the samples of silage obtained from dairyfarms in different districts of Japan and from the laboratory, the author investigated into factors that determine the quality of silage; and the results obtained on the quality of silage in various positions in a silo, the change of quality after the removal of silage from a silo, the relationship between the pH value and the ammoniac nitrogen content, and also the result of appraisal of silage by means of FLIEG'S method are described in this paper. The results are summarized as follows: (1) It is rarely possible to state that the quality of silage is always homogeneous throughout in a silo from the upper to the bottom layers, not to speak of the surface spoilage. On the average, the pH value of silage in the middle portion is lower than that in the outer, although it cannot be stated that the difference is always significant. (2) In winter season, the change of quality of silage is rather little within about a week after the removal of silage from a silo, if the silage is preserved without exposure to air. It is suggested therefore that an amount of silage for a few days use may be removed at a time for the purpose of labor-saving. (3) A significant positive correlation, γ=+0.603, was found between the pH value and the ammonia nitrogen content (p<0.001). (4) Examples of the results of the appraisal by means of FLIEG's method are presented in Table 9. A significant positive correlation has been found between the results of appraisals by means of FLIEG'S method and those obtained by GNEIST'S. When the two series of results of evaluation were different, the values obtained by FLIEG'S method were slightly higher than those by GNEIST'S

Juvenile Idiopathic Arthritis Subtype- and Sex-specific Associations with Genetic Variants in the PSMA6/PSMC6/PSMA3 Gene Cluster

BackgroundThe ubiquitin proteasome system plays an exceptional biological role in the antigen processing and immune response and it could potentially be involved in pathogenesis of many immunity-related diseases, including juvenile idiopathic arthritis (JIA).MethodsThe PSMB5 (rs11543947), PSMA6 (rs2277460, rs1048990), PSMC6 (rs2295826, rs2295827), and PSMA3 (rs2348071) proteasomal genes were genotyped on JIA subtype- and sex-specific association; plasma proteasome levels was measured in patients having risk and protective four-locus genotypes and eventual functional significance of allele substitutions was evaluated in silico.ResultsLoci rs11543947 and rs1048990 were identified as disease neutral and other loci as disease susceptible (p < 0.05). The rs2277460, rs2295826, and rs2295827 loci had the strongest association with oligoarthritis [odds ratio (OR) = 2.024, 95% confidence interval (CI) 1.101–3.722; OR = 2.371, 95% CI 1.390–4.044; OR = 2.183, 95% CI 1.272–2.737, respectively), but the rs2348071 locus was associated with polyarthritis in females (OR = 3.438, 95% CI 1.626–7.265). A strong (p < 0.001) association was detected between the rs2277460/rs2295826/rs2295827/rs2348071 four-locus genotypes and the healthy phenotype when all loci were homozygous on common alleles (OR 0.439, 95% CI 0.283–0.681) and with the disease phenotype when the rs2348071 and the rs2295826 and/or rs2295827 loci were represented by risk genotypes simultaneously (OR 4.674, 95% CI 2.096–10.425). Rarely observed in controls, the double rs2277460/rs2348071 heterozygotes were rather frequent in affected males and more strongly associated with polyarthritis (p < 0.05). Haplotypes carrying the rare rs2295826/rs2295827 and rs2277460 alleles showed a strong (p < 0.001) association with oligo- and polyarthritis, respectively. The plasma proteasome level was found to be significantly higher in females having four-locus risk genotypes compared with protective genotypes (p < 0.001). Sequence affinity to transcription factors and similarity to splicing signals, microRNAs and/or hairpin precursors potentially depend on allele substitutions in disease susceptible loci.ConclusionWe demonstrate for the first time evidence of a sex-specific association of PSMA6/PSMC6/PSMA3 genetic variants with subtypes of JIA and plasma proteasome concentrations. Theoretical models of the functional significance of allele substitutions are discussed

Studies on Silage-Making XI. : The Effects of Adding Morea Liquid Feed or Molasses to Rice Straw to make Silage

糖ミツ尿索液体飼料(Moreaと記す)および糖ミツのイネワラサイレージへの添加効果を知るために,乾燥イネワラの水分を約72%に調節し,5%量のMoreaまたは糖ミツを添加して常法により,サイレージを調製した. でき上りサイレージの成分・品質ならびに消化率を調査して,これらの添加効果を検討した. 実験結果の要約はつぎのようである. 1)各区サイレージのpH価は,Morea区5.18,無添加区4.92,糖ミツ区3.90で,糖ミツ区がもっとも低かった. 2)サイレージの乳酸含量は,糖ミツ区3.13%,Morea区0.94%,対照区0.32%であり,酪酸はいずれの区にもみられた. 品質は糖ミツ区82点,Morea区25点,対照区12点となり,糖ミツ添加の効果が認められた. 3)全窒素に対するアンモニア態窒素の比率は,糖ミツ区(4.6%)はきわめて低く,対照区(41.8%),Morea区(37.5%)はきわめて高かった

Studies on the Chemical Composition and Quality of Silage : XIV. The Influence of Wilting of Grass on the Feeding value of Silage

本実験は,サイレージの発酵的品質と飼料価値との関係を知るための研究の一環として計画したものである. 2番草のイタリアンライグラスを伸長期に破砕しながら収穫し,日乾法によって材科草の乾物を21.7%（DM20区),39.9%(DM40区),60.3%(DM60区)に調節して,それぞれ実験用サイロに埋蔵してサイレージを調製した. なお,DM60区は気密条件で調製した. でき上りサイレージについてヒツジによる消化試験を実施し飼料価値を求めた. 実験結果の要約は次のようである. 1)でき上りサイレージのpH価は,DM20区:3.80,DM40区:4.02,DM60区:4.25であり,評点は,DM20区:90点,DM40区およびDM60区:100点であり,アンモニア態窒素比率は,それぞれ,5.79,5.12,3.72で低い値であった. すなわち,乾物含量の異なる材料からほば近い発酵的品質のサイレージが調製された. 2)ヒツジによる消化試験の結果,粗タンパク質の消化率は日乾の程度の低い区ほど高く,NFEの消化率は日乾の程度の高い区ほど高くなる傾向が認められた. 乾物中のTDNも日乾の程度の高い区ほどわずかに高くなる傾向がうかがえた. しかし,分散分析の結果,各区の各成分の消化率およびDCP,TDN含量間に有意差は認められなかった. 3)本実験の結果などから,日乾の効果は主として発酵的品質の改善を通じて発現されるものであることが推測された