Rapid determination of sulfonamides in millk using micellar electrokinetic chromatography with fluorescence detection.

A new method was developed for the determination of sulfonamides in milk by using micellar electrokinetic chromatography coupled with fluorescence detection. Separation of fluorescamine-derivatized sulfonamides was accomplished by using a buffer 13.32 mM disodium hydrogen phosphate, 6.67 mM potassium dihydrogen phosphate and 40 mM sodium dodecyl sulphate at pH 7.5 in addition to positive power supply at 21 kV at 25 °C. Detection was performed using UG-11 excitation filter and 495 nm emission filters. The proposed capillary electrophoresis method allows the separation of five sulfonamides within 7 min with a limit of detection of 1.59–7.68 nmol/L for all the sulfonamides considered for present study. A simple sample preparation method with fairly good recoveries 85–114% is also presented in current paper. Inter-day and intra-day validation of the separation method shows fairly good results. Robustness of the method has also been studied

Rapid determination of sulfonamides in milk using micellar electrokinetic chromatography with fluorescence detection

A new method was developed for the determination of sulfonamides in milk by using micellar electrokinetic chromatography coupled with fluorescence detection. Separation of fluorescamine-derivatized sulfonamides was accomplished by using a buffer 13.32 mM disodium hydrogen phosphate, 6.67 mM potassium dihydrogen phosphate and 40 mM sodium dodecyl sulphate at pH 7.5 in addition to positive power supply at 21 kV at 25 °C. Detection was performed using UG-11 excitation filter and 495 nm emission filters. The proposed capillary electrophoresis method allows the separation of five sulfonamides within 7 min with a limit of detection of 1.59–7.68 nmol/L for all the sulfonamides considered for present study. A simple sample preparation method with fairly good recoveries 85–114% is also presented in current paper. Inter-day and intra-day validation of the separation method shows fairly good results. Robustness of the method has also been studied. © 2005 Elsevier B.V. All rights reserved

Fast seperation and sensitive detection of carcinogenic aromatic amines by reversed phase liquid chromatography coupled with electrochemical detection.

A micro-LC method was developed for the fast and sensitive analysis of aromatic amines by electrochemical detection. The chromatographic separation of nine carcinogenic aromatic amines was performed on an ABZ + PLUS column with detection limits up to pM L(-1) levels. Mobile phase comprised of methanol-acetate buffer of pH 5 (45:55, v/v) used at a flow rate of 0.2 ml min(-1). The detection was performed with a 6 mm glassy carbon electrode at an applied potential of 0.8 V versus Ag/AgCl. An intraday RSD for retention time and peak area were between 0.22% and 0.73% and 1.86% and 4.03%, respectively. The interdays RSD for retention time and peak area were between 0.47% and 1.35% and 2.04% and 4.42%, respectively. The applicability of the assay has been demonstrated by analyzing these aromatic amines in lake water and synthetic food colour additives. A comparison is given between electrochemical and UV detection