PHYTOCHEMICAL, MICROBIOLOGICAL, AND TECHNOLOGICAL STUDIES IN THE FIELD OF OBTAINING A HYDROALCOHOLIC EXTRACT WITH ANTIMICROBIAL ACTIVITY FROM THE LIQUORICE ROOT

Objective: The objective of this work was to carry out studies in the field of development of a hydroalcoholic extract from the liquorice root that has a high level of antimicrobial activity.Methods: For the antibacterial study of extracts, we have used the agar well diffusion method. In our research, we have utilized six test-strain microorganisms: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Proteus vulgaris ATCC 4636, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 885/653, and Bacillus subtilis ATCC 6633. Authors have applied vector theory for antimicrobial activity comparison of liquorice root extracts.Results: Phytochemical composition and antimicrobial activity of hydroalcoholic extracts from the liquorice root with the use of different concentrations of ethanol have been studied. The optimal range of ethanol concentration of 65±15% v/v has been found. The dependency between the integral index of antimicrobial activity of the extract and concentration of glycyram and licuroside has been found. The minimal concentration of glycyram and licuroside in the extract has been calculated for an exhibition of the target level of extract's antimicrobial activity, which is 1.0 and 0.11% w/v, respectively.Conclusion: It has been found that the concentration of licuroside has greater influence on the integral index of extract's antimicrobial activity in comparison with that one of glycyram. A highly effective technology for obtaining an extract with target phytochemical parameters has been suggested

Modeling of solvent effects on phytocompounds' extraction from glycyrrhizae radix

The aim of the article was to study and model solvent effects on phytocompounds' extraction from Glycyrrhizae radix for substantiation of rational choice of the extractant in the technology of drugs obtained from this type of plant raw material. The process of extraction was carried out by simple maceration for 24 h at temperature 25°C ± 1°C and extractant/plant raw material ratio 5:1 (v/w). The content of hydro-ethanolic extracts based on ethanol solutions with concentration of 22, 41, 50, 71, 82, and 96% ± 1% v/v and some other solvents was studied by reversed phase high-performance liquid chromatography. The optimal range of solvent concentration for simultaneous extraction of chalcones and glycyrrhizic acid derivatives from Glycyrrhizae radix was determined. It was found that dielectric constant of the solvent plays a key role in the distribution process of phytocompounds between the phases; there is a certain range of dielectric constant values of the solvent-water solution, within which maximum phytocompound concentration in the extract can be observed; the dependency between phytocompound concentration in the extract and dielectric constant of the solvent-water solution can be described by equation lnC = a + b/ε+d/ε2