National Primary Care Day \u2797

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Tropidosteptes forestierae (Hemiptera: Heteroptera: Miridae: Mirinae): a new species of Plant Bug injuring ornamental Florida Swampprivet, Forestiera segregata (Oleaceae), in South Florida

The mirine plant bug Tropidosteptes forestierae, new species (Hemiptera: Miridae) is described from Collier County, Florida, where it was found causing serious injury to an extensive ornamental hedge of Florida swampprivet, Forestiera segregata (Jacq.) Krug and Urb. (Oleaceae). Adult male and female, fifth instar, and egg are described. Color images of the adults, nymph, egg, and injury; scanning photomicrographs of selected adult structures; and illustrations of male genitalia are provided. A key to help distinguish the 16 species of Tropidosteptes known to occur in the southeastern United States is given

Wolves and Bears: An Interdisciplinary Activity Guide for Grades 3-5

A popular set of Discovery Boxes are used by educators across North Central Washington. These boxes are administered by the U.S. Forest Service and focus on such themes as wolves, bears, fire, plants and forests. These boxes are available to be checked out to educators for two-week periods, and reach approximately 9,000 students annually. Each box contains hands-on props which enhance the learning of each theme. Some of the boxes have activity guides in them to assist the educator in teaching the theme and thus using the box to its full potential. Two of the most popular boxes, the wolf and bear box, currently do not have an activity guide. The purpose of this project was to develop activity guides for the bear and wolf boxes owned by the Leavenworth Ranger District, U.S. Forest Service. The activities developed for this project incorporated aspects of the bears and wolves of Washington State and are to be used with the bear and wolf boxes. The activity guides were targeted for grades 3-6, the most popular grades that use the box. The guides are interdisciplinary in nature. Background information about bears and wolves were incorporated into the guides and each guide was aligned with the Washington State Essential Academic Learning Requirements. The lessons were reviewed by four North Central Washington teachers (grades 3-5), tested in the classroom for their usefulness, and modified as needed. i

Plasticity of plasma membrane compartmentalization during plant immune responses

Plasma membranes require high levels of plasticity to modulate the perception and transduction of extracellular and intracellular signals. Dynamic lateral assembly of protein complexes combined with an independent compositional lipid patterning in both membrane leaflets provide cells the opportunity to decorate this interface with specific proteins in an organized but dynamic manner. Such ability to dynamically reorganize the protein content of the plasma membrane is essential for the regulation of processes such as polarity of transport, development, and microbial infection. While the plant cell wall represents the first physical and mostly unspecific barrier for invading microbes, the plasma membrane is at the forefront of microbial recognition and initiation of defense responses. Accumulating evidence indicating dynamic compartmentalization of plasma membranes in response to environmental cues has increased the interest in the compositional heterogeneity of this bilayer. Here, we elucidate the recruitment of specific proteins into defined membrane structures that ensure functional compartmentalization of the bilayer during infection processes

Ultrasonic Evaluation of Low Density Powder Compacts

Ultrasonic evaluation of powder compacts provides valuable information about the density of the powder and incipient defects in the compact. Since the specimens would be contaminated if they were inspected with traditional immersion or contact techniques, procedures to dry couple or vacuum bag and immerse the powder samples were implemented. With these methods ultrasonic energy was propagated through the powder compacts and the acoustic velocity and attenuation were measured. The ability to immerse the powder samples not only provides consistent coupling but allows scanning which is a valuable tool to image variations and defects in the compacts

Use of probiotic Lactobacillus preparation to prevent diarrhoea associated with antibiotics: randomised double blind placebo controlled trial

Objective To determine the efficacy of a probiotic drink containing Lactobacillus for the prevention of any diarrhoea associated with antibiotic use and that caused by Clostridium difficile. Design Randomised double blind placebo controlled study. Participants 135 hospital patients (mean age 74) taking antibiotics. Exclusions included diarrhoea on admission, bowel pathology that could result in diarrhoea, antibiotic use in the previous four weeks, severe illness, immunosuppression, bowel surgery, artificial heart valves, and history of rheumatic heart disease or infective endocarditis. Intervention Consumption of a 100 g (97 ml) drink containing Lactobacillus casei, L bulgaricus, and Streptococcus thermophilus twice a day during a course of antibiotics and for one week after the course finished. The placebo group received a longlife sterile milkshake. Main outcome measures Primary outcome: occurrence of antibiotic associated diarrhoea. Secondary outcome: presence of C difficile toxin and diarrhoea. Results 7/57 (12%) of the probiotic group developed diarrhoea associated with antibiotic use compared with 19/56 (34%) in the placebo group (P=0.007). Logistic regression to control for other factors gave an odds ratio 0.25 (95% confidence interval 0.07 to 0.85) for use of the probiotic, with low albumin and sodium also increasing the risk of diarrhoea. The absolute risk reduction was 21.6% (6.6% to 36.6%), and the number needed to treat was 5 (3 to 15). No one in the probiotic group and 9/53 (17%) in the placebo group had diarrhoea caused by C difficile (P=0.001). The absolute risk reduction was 17% (7% to 27%), and the number needed to treat was 6 (4 to 14). Conclusion Consumption of a probiotic drink containing L casei, L bulgaricus, and S thermophilus reduce the incidence of antibiotic associated diarrhoea and C difficile associated diarrhoea. This has the potential to decrease morbidity, healthcare costs, and mortality if used routinely in patients aged over 50

Cloning of terminal transferase cDNA by antibody screening

A cDNA library was prepared from a terminal deoxynucleotidyltransferase-containing thymoma in the phage vector λgt11. By screening plaques with anti-terminal transferase antibody, positive clones were identified of which some had β-galactosidase-cDNA fusion proteins identifiable after electrophoretic fractionation by immunoblotting with anti-terminal transferase antibody. The predominant class of cross-hybridizing clones was determined to represent cDNA for terminal transferase by showing that one representative clone hybridized to a 2200-nucleotide mRNA in close-matched enzyme-positive but not to enzyme-negative cells and that the cDNA selected a mRNA that translated to give a protein of the size and antigenic characteristics of terminal transferase. Only a small amount of genomic DNA hybridized to the longest available clone, indicating that the sequence is virtually unique in the mouse genome

Evaluating the most promising sites for wind energy development in Arizona USA: Working paper series--05-09

During the summer of 2003, the state of Arizona took delivery of a set of high-resolution wind energy maps. After applying various exclusions, the developable wind energy potential is 23,290 MW of class 3 or higher, 2,630 MW of class 4 or higher, and 775 MW of class 5 or higher winds. Having determined the potential wind resource, the geographical information system data supplied with the wind maps was used to create a wind resource inventory and to systematically identify the most promising sites for wind energy development. In addition to wind energy potential, proximity to transmission lines and roads, and land ownership were considered in this analysis. Following that, the cost of energy was estimated at a few geographically diverse sites, including class 3, 4, and 5 wind resource areas, at a hub height of 70 m. These estimates revealed that the real levelized cost of energy in 2005 dollars ranged from 4.21 to 5.04 cents per kWh, as the wind class varies from 5 to 3. This paper documents the findings of the wind mapping process, describes the method and results of evaluating the most promising sites for wind development, and presents the cost of energy results