Management of single-rooted maxillary central incisor with two canals: A case report

The aim of this study was to report the endodontic management of right maxillary central incisor having two canals with Vertucci canal configuration type IV. Internal morphology of root canals is variable and often complex. Therefore, to achieve a technically satisfactory endodontic outcome, the clinician must have adequate knowledge of the internal canal morphology and its variations in order to debride and obturate the root canal system thoroughly

A cross sectional study on depression among paramedical students in MES Institute of Paramedical Sciences

Background: Depression is characterized by persistent sadness and a lack of interest or pleasure in previously rewarding or enjoyable activities. It is very important to identify the factors predisposing to depression among paramedical students. The main objective of this study was to assess the prevalence of depression and the factors associated with depression among the paramedical students. Methods: A cross-sectional study was done among all the 208 paramedical students studying in MES using a predesigned questionnaire along with Beck’s depression inventory tool to identify depression.  Chi-square test and multivariate regression analysis were used to find the association between variables. Results: The prevalence of depression was found to be 24.5%. 11.54 % of the total study participants have borderline depression, while 10.58%, 1.92% and 0.48 % had moderate, severe and extreme form of depression respectively. Chi square test showed type of family, course of study, smoking habits, traumatic events in the past, bad interpersonal relationship, concern regarding the future were found to be statistically associated with depression. In multivariate analysis, bad interpersonal relationship between friends and family members (OR= 3.13), worrying about the future (OR=6.03) and those who never sought any form of help for mental stress (OR=1.5) were the risk factors identified. Conclusions: Course selection, type of family they came from, any traumatic events in the past, lack of interpersonal relationship between friends, worrying about the job opportunities after completing the course, not seeking help for mental stress were the factors contributing towards depression

Automated, image-based quantification of peroxisome characteristics with perox-per-cell.

SUMMARY: perox-per-cell automates cumbersome, image-based data collection tasks often encountered in peroxisome research. The software processes microscopy images to quantify peroxisome features in yeast cells. It uses off-the-shelf image processing tools to automatically segment cells and peroxisomes and then outputs quantitative metrics including peroxisome counts per cell and spatial areas. In validation tests, we found that perox-per-cell output agrees well with manually quantified peroxisomal counts and cell instances, thereby enabling high-throughput quantification of peroxisomal characteristics. AVAILABILITY AND IMPLEMENTATION: The software is coded in Python. Compiled executables and source code are available at https://github.com/AitchisonLab/perox-per-cell. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online

Successful root canal treatment of an atypical mandibular canine with a boomerangshaped root and canal system with a large periapical lesion—A case report with 9-year follow-up

Aim This report describes an atypical mandibular canine with a single boomerang-shaped root and root canal system with a large periapical lesion managed by root canal treatment. Methods The chief complaint of a 16-year-old male was acute pain and an abnormal sensation in the right mandibular canine (#27). The clinical examination revealed that tooth #27 had an unusual coronal morphology. The buccal aspect of the tooth resembled that of a normal canine but was significantly broader than expected. On the lingual aspect of the crown, however, there was an unusual cusp-like structure with ridges that was slightly less prominent than the buccal incisal tip. The intraoral periapical radiographs revealed a complex root with an obvious cow horn-shaped canal mesially and distally, but with the suggestion of a root structure joining the mesial and distal extensions. A large periapical lesion was present, Computed tomography revealed the presence of a single root and canal system shaped like a boomerang. Root canal treatment was performed, and the patient was followed-up for 9 years. Results Following root canal treatment, the patient had no symptoms, no mobility, no periodontal pockets, or root resorption. At 9 years, the cone beam computed tomography images confirmed that satisfactory healing of the periapical tissues had occurred. Conclusion The mandibular right canine had a unique boomerang-shaped root and canal system. Effective shaping and cleaning of the complex canal shape plus thermoplastic root filling aided the successful healing of the periapical lesion

PRIDASE 2024 guidelines for reporting diagnostic accuracy studies in endodontics: Explanation and elaboration

The Preferred Reporting Items for Diagnostic Accuracy Studies in Endodontics (PRIDASE) 2024 guidelines are based on the Standards for Reporting of Diagnostic Accuracy Studies (STARD) 2015 guidelines and the Clinical and Laboratory Images in Publications (CLIP) principles, with the addition of items specifically related to endodontics. The use of the PRIDASE 2024 guidelines by authors and their application by journals during the peer review process will reduce the possibility of bias and enhance the quality of future diagnostic accuracy studies. The PRIDASE 2024 guidelines consist of a checklist containing 11 domains and 66 individual items. The purpose of the current document is to provide an explanation for each item on the PRIDASE 2024 checklist, along with examples from the literature to help readers understand their importance and offer advice to those developing manuscripts. A link to the PRIDASE 2024 explanation and elaboration document is available on the Preferred Reporting Items for study Designs in Endodontology (PRIDE) website (https://pride‐endodonticguidelines.org/pridase/) and on the International Endodontic Journal website (https://onlinelibrary.wiley.com/page/journal/13652591/homepage/pride‐guidelines.htm)

PRILE 2021 guidelines for reporting laboratory studies in Endodontology: explanation and elaboration

Guidance to authors is needed to prevent their waste of talent, time and resources in writing manuscripts that will never be published in the highest-quality journals. Laboratory studies are probably the most common type of endodontic research projects because they make up the majority of manuscripts submitted for publication. Unfortunately, most of these manuscripts fail the peer-review process, primarily due to critical flaws in the reporting of the methods and results. Here, in order to guide authors, the Preferred Reporting Items for study Designs in Endodontology (PRIDE) team developed new reporting guidelines for laboratory-based studies: the Preferred Reporting Items for Laboratory studies in Endodontology (PRILE) 2021 guidelines. The PRILE 2021 guidelines were developed exclusively for the area of Endodontology by integrating and adapting the modified CONSORT checklist of items for reporting in vitro studies of dental materials and the Clinical and Laboratory Images in Publications (CLIP) principles. The process of developing the PRILE 2021 guidelines followed the recommendations of the Guidance for Developers of Health Research Reporting Guidelines. The aim of the current document is to provide authors with an explanation for each of the items in the PRILE 2021 checklist and flowchart with examples from the literature, and to provide advice from peer-reviewers and editors about how to solve each problem in manuscripts prior to their peer-review. The Preferred Reporting Items for study Designs in Endodontology (PRIDE) website (http://pride-endodonticguidelines.org/prile/) provides a link to the PRILE 2021 explanation and elaboration document as well as to the checklist and flowchart