Rapid determination of sulfonamides in milk using micellar electrokinetic chromatography with fluorescence detection

A new method was developed for the determination of sulfonamides in milk by using micellar electrokinetic chromatography coupled with fluorescence detection. Separation of fluorescamine-derivatized sulfonamides was accomplished by using a buffer 13.32 mM disodium hydrogen phosphate, 6.67 mM potassium dihydrogen phosphate and 40 mM sodium dodecyl sulphate at pH 7.5 in addition to positive power supply at 21 kV at 25 °C. Detection was performed using UG-11 excitation filter and 495 nm emission filters. The proposed capillary electrophoresis method allows the separation of five sulfonamides within 7 min with a limit of detection of 1.59–7.68 nmol/L for all the sulfonamides considered for present study. A simple sample preparation method with fairly good recoveries 85–114% is also presented in current paper. Inter-day and intra-day validation of the separation method shows fairly good results. Robustness of the method has also been studied. © 2005 Elsevier B.V. All rights reserved

Rapid determination of sulfonamides in millk using micellar electrokinetic chromatography with fluorescence detection.

A new method was developed for the determination of sulfonamides in milk by using micellar electrokinetic chromatography coupled with fluorescence detection. Separation of fluorescamine-derivatized sulfonamides was accomplished by using a buffer 13.32 mM disodium hydrogen phosphate, 6.67 mM potassium dihydrogen phosphate and 40 mM sodium dodecyl sulphate at pH 7.5 in addition to positive power supply at 21 kV at 25 °C. Detection was performed using UG-11 excitation filter and 495 nm emission filters. The proposed capillary electrophoresis method allows the separation of five sulfonamides within 7 min with a limit of detection of 1.59–7.68 nmol/L for all the sulfonamides considered for present study. A simple sample preparation method with fairly good recoveries 85–114% is also presented in current paper. Inter-day and intra-day validation of the separation method shows fairly good results. Robustness of the method has also been studied

Determination of Biogenic Amines in Lake Water by Micellar Electrokinetic Chromatography with Fluorescence Detection after Derivatization with Fluorescamine1.

A simple and rapid method has been developed for the determination of biogenic amines in lake water using micellar electrokinetic chromatography with fluorescence detection. Separation of fluorescam�ine derivatized biogenic amines was accomplished by using borate buffer of pH 9.5 containing 40 mM of sodium dodecyl sulphate. The method has been optimized with respect to fluorescamine concentration, reaction pH, reaction time, separation voltage and injection time. Detection was performed by using UG�11 excitation filter and 495 nm emission filter. The proposed method for histamine, tyramine and dopamine allowed their separation within 2 min with detection limits in nM range. The interday and intraday reproduc� ibility of peak areas were less than 6.5%. Recovery of spiked samples was 95.76–116.31%

Synthesis and characterization of Poly (N- Isopropylacrylamide) ZnO nanocomposites for textile applications

High performance polymers exhibiting multifunctional characteristics can be achieved by the introduction of inorganic nanoparticles like ZnO(Zinc oxide) into the functional polymers. In the present work a copolymer epoxy poly (dimethylacrylamide) was synthesized to disperse the ZnO nanoparticles. The aim of the work is to develop a new method/process/material for the dispersion of nanoparticles and evaluation of the performance of these composites. FT-IR studies of the polymer adsorbed ZnO nanoparticles confirmed that the polymer molecules chain were anchored on the surface of the ZnO nanoparticles. The improved interfacial interaction between the particles and polymer enhanced the thermal properties of the composites. The results also show that the newly prepared polymer composite matrix uniformly has the ability to disperse the ZnO nanoparticles well as evidenced by SEM analysis, and the particles almost remain in their original shape and size even after incorporation into the polymer matrix. Nevertheless, it was also found by dynamic light scattering analysis that the mean particle size of the dispersion was increased with increasing ZnO content. The results were consistent with SEM observations. The value of zeta potential results, show how the Poly (N–isopropylacryl amide (PNIPAM) can adsorb onto the ZnO nanoparticles and impart – ve charge to the surface of the nanoparticles.Department of Science and Technology (DST), India and Fundação para a Ciência e a Tecnologia (FCT), Portugal