Trichosanthes kirilowii

Trichosanthes kirilowii tuber is a traditional medicine which exhibits various medicinal effects including antidiabetic and anticancer activities in several cancer cells. Recently, it was reported that Cucurbitacin D (CuD) isolated from Trichosanthes kirilowii also induces apoptosis in several cancer cells. Constitutive signal transducer and activator of transcription 3 (STAT3), which is an oncogenic transcription factor, is often observed in many human malignant tumor, including breast cancer. In the present study, we tested whether Trichosanthes kirilowii ethanol extract (TKE) or CuD suppresses cell growth and induces apoptosis through inhibition of STAT3 activity in breast cancer cells. We found that both TKE and CuD suppressed proliferation and induced apoptosis and G2/M cell cycle arrest in MDA-MB-231 breast cancer cells by inhibiting STAT3 phosphorylation. In addition, both TKE and CuD inhibited nuclear translocation and transcriptional activity of STAT3. Taken together, our results indicate that TKE and its derived compound, CuD, could be potent therapeutic agents for breast cancer, blocking tumor cell proliferation and inducing apoptosis through suppression of STAT3 activity

Herbal Extract SH003 Suppresses Tumor Growth and Metastasis of MDA-MB-231 Breast Cancer Cells by Inhibiting STAT3-IL-6 Signaling

Cancer inflammation promotes cancer progression, resulting in a high risk of cancer. Here, we demonstrate that our new herbal extract, SH003, suppresses both tumor growth and metastasis of MDA-MB-231 breast cancer cells via inhibiting STAT3-IL-6 signaling path. Our new herbal formula, SH003, mixed extract from Astragalus membranaceus, Angelica gigas, and Trichosanthes kirilowii Maximowicz, suppressed MDA-MB-231 tumor growth and lung metastasis in vivo and reduced the viability and metastatic abilities of MDA-MB-231 cells in vitro. Furthermore, SH003 inhibited STAT3 activation, which resulted in a reduction of IL-6 production. Therefore, we conclude that SH003 suppresses highly metastatic breast cancer growth and metastasis by inhibiting STAT3-IL-6 signaling path

Saussurea lappa clarkeDerived costunolide prevents TNF alpha induced breast cancer cell migration and invasion by inhibiting NF-

Saussurea lappa Clarke (SLC) has been used as a traditional medicine in Korea, China, and Japan for the treatment of abdominal pain and tenesmus. Costunolide, a sesquiterpene lactone isolated from SLC, has diverse medicinal effects. However, the anticancer effects of costunolide are still unclear in breast cancer. In this study, we demonstrate that costunolide suppresses tumor growth and metastases of MDA-MB-231 highly metastatic human breast cancer cells via inhibiting TNF -induced NF-B activation. Costunolide inhibited MDA-MB-231 tumor growth and metastases without affecting body weights in the in vivo mouse orthotopic tumor growth assays. In addition, costunolide inhibited in vitro TNF -induced invasion and migration of MDA-MB-231 cells. Costunolide further suppressed TNF -induced NF-B signaling activation, resulting in a reduced expression of MMP-9, a wellknown NF-B-dependent gene to mediate breast cancer cell growth and metastases. Therefore, we conclude that SLC and its derivative costunolide suppress breast cancer growth and metastases by inhibiting TNF -induced NF-B activation, suggesting that costunolide as well as SLC may be promising anticancer drugs, especially for metastatic breast cancer

Dual Fistulas of Ascending Aorta and Coronary Artery to Pulmonary Artery

Coronary artery fistula to pulmonary artery is common. However, to the best of our knowledge, a case of coronary artery fistula to pulmonary artery associated with aortopulmonary fistula remains unreported. We herein report a 64-year-old female with a left anterior descending coronary artery and ascending aorta to pulmonary artery fistulas, and conduct a brief review of the literature

Effect of Sipjeondaebo-Tang on Cancer-Induced Anorexia and Cachexia in CT-26 Tumor-Bearing Mice

Cancer-associated anorexia and cachexia are a multifactorial condition described by a loss of body weight and muscle with anorexia, asthenia, and anemia. Moreover, they correlate with a high mortality rate, poor response to chemotherapy, poor performance status, and poor quality of life. Cancer cachexia is regulated by proinflammatory cytokines such as interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor-α (TNF-α). In addition, glucagon like peptide-1 (GIP-1), peptide YY (PYY), ghrelin, and leptin plays a crucial role in food intake. In this study, we investigated the therapeutic effects of one of the traditional herbal medicines, Sipjeondaebo-tang (Juzen-taiho-to in Japanese; SJDBT), on cancer anorexia and cachexia in a fundamental mouse cancer anorexia/cachexia model, CT-26 tumor-bearing mice. SJDBT was more significantly effective in a treatment model where it was treated after anorexia and cachexia than in a prevention model where it was treated before anorexia and cachexia on the basis of parameters such as weights of muscles and whole body and food intakes. Moreover, SJDBT inhibited a production of IL-6, MCP-1, PYY, and GLP-1 and ameliorated cancer-induced anemia. Therefore, our in vivo studies provide evidence on the role of SJDBT in cancer-associated anorexia and cachexia, thereby suggesting that SJDBT may be useful for treating cancer-associated anorexia and cachexia

An Intelligent Process to Estimate the Nonlinear Behaviors of an Elasto-Plastic Steel Coil Damper Using Artificial Neural Networks

This study developed a nonlinear behavior prediction model for elasto-plastic steel coil dampers (SCDs) using artificial neural networks (ANN). To train the ANN, first, the input and output data of the behavior of the elasto-plastic SCD was prepared. This study utilized the design parameters and load–displacement curves of the SCD to train the ANN. The elasto-plastic load–displacement curve of the SCD was obtained from simulation results using an ANSYS workbench. The design parameters (wire diameter, internal diameter, number of active windings, yield strength) of the SCD were defined as the input patterns, while the yield deformation, first stiffness, and second stiffness were output patterns. During learning of the neural network model, 60 datasets of the SCD were used as the learning pattern, and the remaining 21 were used to verify the model. Although this study used a small number of learning patterns, the ANN predicted accurate results for yield displacement, first stiffness, and second stiffness. In this study, the ANN was found to perform very well, predicting the nonlinear response of the SCD, compared with the values obtained from a finite element analysis program

Effect of Frequency Content of Earthquake on the Seismic Response of Interconnected Electrical Equipment

To ensure the stable operation of safety-related nuclear power plant (NPP) equipment, they are tested by following the seismic qualification procedures. The in-cabinet response spectrum (ICRS) is used to test the mounted components. However, the ICRS varies significantly with the number of uncertainties that include (1) loaded and unloaded condition of the cabinets, (2) the number of connected cabinets (grouping effects), and (3) higher frequency contents in the seismic inputs. This study focuses on the ICRS generation and alteration induced due to the listed uncertainties. A prototype of an electrical cabinet was experimentally examined. Followed by the numerical modeling of the cabinet, the seismic analysis for the group of cabinets was performed using artificial ground motion compatible with the standard design spectrum and the real accelerograms of high and low frequency contents. The seismic response using finite element (FE) analysis manifests (1) natural frequency of loaded cabinets reduced due to the in-cabinet components while for the unloaded cabinets it increased significantly, (2) a consistent reduction in ICRS due to the grouping effect was recorded when excited by the lower-frequency motion, while it was amplified dramatically due to high-frequency pulses. Interconnected cabinets under the low-frequency input motions have a significant reduction of 50% in the ICRS that corresponds to the higher stiffness of the cabinets, while a 100% increase under the high frequency of ground motion was obtained. High frequency of ground motion, usually above 10 Hz, can cause the interconnected cabinets to resonate as the natural frequency of these equipment lies in this range