Optimalne potrebe juvenilnih morskih grgeča sebastes schlegeli u proteinima i lipidima

U ovom istraživanju su analizirane optimalne potrebe juvenilnih morskih grgeča Sebastes schlegeli u proteinima i lipidima. 810 juvenilnih riba je izabrano po principu slučajnosti i distribuirano u 27 tankova od po 50 L sa protočnih sistemom. Pripremljeno je 9 eksperimentalnih smeša u vidu 3x3 faktorijalne eksperimentalne postavke: tri nivoa proteina (45, 50 i 55%) x tri nivoa lipida (11, 15 i 19%). Nivo proteina je imao uticaj na prirast riba, dok nivo lipida nije. Prirast riba hranjenih smešom u odnosu 50P-15L (50% proteina i 15% lipida) je bio veći nego prirast riba hranjenih smešama sa 45% proteina, bez obzira na nivo lipida, ali je bio isti kao kod riba hranjenih sa smešama 50P-11L, 50P-19L, 55P-11L, 55P-15L i 55P-19L. Stopa efikasnosti hrane (FER) riba je bila pod uticajem proteina u hrani ali ne i nivoa lipida. Stopa efikasnosti proteina (PER) riba je takođe bila pod uticajem proteina u hrani ali ne i nivoa lipida. Može se zaključiti da je za juvenilne Sebastes schlegeli optimalan nivo proteina i lipida za dobar prirast i iskoristljivost hrane (PER and NRE) 50% i 15% odnosno 45% i 19%, dok je optimalan odnos proteina i energije 27.4 i 23.9 mg protein/kJ

Role of G{alpha}12 and G{alpha}13 as Novel Switches for the Activity of Nrf2, a Key Antioxidative Transcription Factor

G{alpha}12 and G{alpha}13 function as molecular regulators responding to extracellular stimuli. NF-E2-related factor 2 (Nrf2) is involved in a protective adaptive response to oxidative stress. This study investigated the regulation of Nrf2 by G{alpha}12 and G{alpha}13. A deficiency of G{alpha}12, but not of G{alpha}13, enhanced Nrf2 activity and target gene transactivation in embryo fibroblasts. In mice, G{alpha}12 knockout activated Nrf2 and thereby facilitated heme catabolism to bilirubin and its glucuronosyl conjugations. An oligonucleotide microarray demonstrated the transactivation of Nrf2 target genes by G{alpha}12 gene knockout. G{alpha}12 deficiency reduced Jun N-terminal protein kinase (JNK)-dependent Nrf2 ubiquitination required for proteasomal degradation, and so did G{alpha}13 deficiency. The absence of G{alpha}12, but not of G{alpha}13, increased protein kinase C {delta} (PKC {delta}) activation and the PKC {delta}-mediated serine phosphorylation of Nrf2. G{alpha}13 gene knockout or knockdown abrogated the Nrf2 phosphorylation induced by G{alpha}12 deficiency, suggesting that relief from G{alpha}12 repression leads to the G{alpha}13-mediated activation of Nrf2. Constitutive activation of G{alpha}13 promoted Nrf2 activity and target gene induction via Rho-mediated PKC {delta} activation, corroborating positive regulation by G{alpha}13. In summary, G{alpha}12 and G{alpha}13 transmit a JNK-dependent signal for Nrf2 ubiquitination, whereas G{alpha}13 regulates Rho-PKC {delta}-mediated Nrf2 phosphorylation, which is negatively balanced by G{alpha}12

A collagen gel-coated, aligned nanofiber membrane for enhanced endothelial barrier function

Herein, a collagen gel-coated and aligned nanofiber membrane named Col-ANM is developed, which remarkably improves endothelial barrier function by providing biochemical and topographical cues simultaneously. Col-ANM is fabricated by collagen gel coating process on an aligned polycaprolactone (PCL) nanofiber membrane, which is obtained by a simple electrospinning process adopting a parallel electrode collector. Human umbilical vein endothelial cells (HUVECs) cultured on Col-ANM exhibit remarkably enhanced endothelial barrier function with high expression levels of intercellular junction proteins of ZO-1 and VE-cadherin, a high TEER, and a cellular permeability compared with the artificial porous membranes in commercial cell culture well inserts. The enhanced endothelial barrier function is conjectured to be attributed to the synergistic effects of topographical and biochemical cues provided by the aligned PCL nanofibers and collagen gel in the Col-ANM, respectively. Finally, the reactive oxygen species is applied to the HUVEC monolayer formed on the Col-ANM to destroy the tight junctions between HUVECs. The destruction of the tight junctions is demonstrated by the decreased TEER value overtime. Results indicate the potential of Col-ANM in modeling endothelial barrier dysfunction-related diseases.11Ysciescopu

CO-COMBUSTION OF KOREAN ANTHRACITE WITH VARIOUS FUELS IN A COMMERCIAL CIRCULATING FLUIDIZED BED BOILER

The effect of co-combustion of various fuels such as bituminous coal, imported anthracite, RDF and wood pellet with Korean anthracite on the combustion and environmental performance was observed in the commercial CFB boiler. The temperatures in the furnace and cyclones exits decreased with increasing the cocombustion ratio of the bituminous coal, which could achieve more stable operation of the CFB boiler. During Co-combustion of the RDF and wood pellets, the temperature of the furnace exit increased slightly with due to volatiles re-combustion which could restrict to increase the co-combustion ratio of the RDF and wood pellets in the CFB boiler. It was limited for the electrostatic precipitator (EP) to maintain the stable operation above 5% of the RDF co-combustion ratio according to decrease of the output voltages of the EP collecting plate. High content of CaO in the RDF and the wood pellet made the required limestone flow rates decrease. The emissions NOx, HCl and dioxin during co-combustion of the RDF and wood pellets did not change appreciably when compared with firing only Korean anthracite, which were also low enough to meet Korean regulation limits. On the other hand, chlorine content in the ashes emitted from the boiler increased gradually with increasing the RDF co-combustion ratio because of absorption by limestone. The co-combustion of various fuels with Korean anthracite in the commercial CFB boiler was found to be of great use up to a certain co-combustion ratio of each fuel without the technical and environmental problems

Effect of Adenotonsillectomy on Attention in Korean Children With Sleep-Disordered Breathing

Objectives Pediatric sleep-disordered breathing (SDB) is a common debilitating disorder that can adversely affect the attention and academic performance of school-age children. Unfortunately, only a few studies have examined the effect of SDB treatment on attention in pediatric populations. Therefore, the aim of this study was to prospectively investigate the effect of SDB treatment on attention in children. Methods This study consecutively enrolled SDB children with adenotonsillar hypertrophy. All subjects underwent standard-of-care treatment (adenotonsillectomy or close observation) and were evaluated using a computerized comprehensive attention test at the initial visit. Comprehensive attention tests consisted of both sustained and divided attention tasks. Each completed task was assigned an attention score, which was based on the number of omission or commission errors. The comprehension attention test was repeated 1 year later. Results A total of 171 children who underwent adenotonsillectomy and 32 children who did not undergo adenotonsillectomy were included in this study. At baseline, there was no significant difference according to the score of all type comprehension attention tests between children in the adenotonsillectomy group and in the observation group. One year after treatment, children in the adenotonsillectomy group had significantly improved scores in all attention tasks. Children in the observation group had only significant improvement in omission errors on sustained attention tasks. Meanwhile, the attention score based on commission errors of divided attention tasks was significantly worse than at baseline for those. Conclusion Our study showed that adenotonsillectomy may be helpful in improving attention in children with SDB

μ-Aqua-κ2 O:O-di-μ-4-methyl­benzoato-κ4 O:O′-bis­[(4-methyl­benzoato-κO)(1,10-phenanthroline-κ2 N,N′)nickel(II)]

In the title dinuclear complex, [Ni2(C8H7O2)4(C12H8N2)2(H2O)], each NiII atom is six-coordinated by three carboxylate O atoms from three 4-methyl­benzoate ligands, two N atoms from two 1,10-phenanthroline ligands, and one μ2-bridging aqua ligand. The dimeric complex is located on a crystallographic twofold axis and each Ni atom displays a distorted octa­hedral coordination geometry. The crystal structure is stabilized via intra­molecular hydrogen bonding of the bridging water mol­ecule and the uncoordinated carboxyl­ate O atoms, and by C—H⋯O and π–π stacking inter­actions [centroid–centroid distances between neighbouring phenanthroline ring systems and between the benzene ring of a 4-methyl­benzoate unit and a phenanthroline ring system are 3.662 (2) and 3.611 (3) Å, respectively]

CD44 Disruption Attenuates Murine Hepatic Ischemia/Reperfusion Injury

Neutrophil adhesion and migration are critical in hepatic ischemia/reperfusion (I/R) injury. Despite very strong preclinical data, recent clinical trials failed to show a protective effect of anti-adhesion therapy in reperfusion injury. Therefore, the aim of this study was to assess the role of CD44 in neutrophil infiltration and liver injury from hepatic I/R. In this study, using a partial hepatic ischemic model in vivo, we determined the potential role of CD44 in neutrophil infiltration and liver injury from I/R. Reperfusion caused significant hepatocellular injury as it was determined by plasma ALT levels and liver histopathology. The injury was associated with a marked neutrophil recruitment and CD44 expression into the ischemic livers. Administration of anti-CD44 antibody to mice reduced the infiltration of neutrophil into the ischemic tissue, associated with liver function preservation. These results support crucial roles of CD44 in neutrophil recruitment and infiltration leading to liver damage in hepatic I/R injury. Moreover, they provide the rationale for targeting to CD44 as a potential therapeutic approach in liver I/R injury