Comment on "Stellar activity masquerading as planets in the habitable zone of the M dwarf Gliese 581"

This document is the Accepted Manuscript Version of the following article: Guillem Anglada-Escude and Mikko Tuomi, 'Comment on "Stellar activity masquerading as planets in the habitable zone of the M dwarf Gliese 581"', Science, Vol 347 (6226), 2015, the final, published version is available online at doi: 10.1126/science.1260796. © 2015 The American Association for the Advancement of Science. All rights reserved.Robertson et al. (Reports, 25 July 2014, p. 440) claimed that activity-induced variability is responsible for the Doppler signal of the proposed planet candidate GJ 581d. We point out that their analysis using periodograms of residual data is inappropriate and promotes inadequate tools. Because the claim challenges the viability of the method to detect exo-Earths, we encourage reanalysis and a deliberation on what the field-standard methods should be.Peer reviewedFinal Accepted Versio

Exploring islands of stability in the design space of cylindrical shell structures

<p><b>Fed-batch fermentation of glucose by <i>R</i>. <i>ornithinolytica</i> B6 with an agitation speed of 200 rpm;</b> (a) without pH control; (b) with pH maintained at 7.0; (c), pH controlled at 5.5 after pH dropped to 5.5 from the initial pH of 7.0.; ■, glucose; □, growth; ♢, ethanol; ●, acetoin; ○, 2,3-BD; ♦, acetic acid; ▲, succinic acid; △, lactic acid; +, butyric acid.</p

MOESM1 of RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain

Additional file 1: Table S1. Oligonucleotides used for gene cloning in this study. Table S2. Oligonucleotides used for RT-PCR and qRT-PCR in this study. Figure S1. Gel images of Fig. 2. Figure S2. Gel images of Fig. 4. Figure S3. Relative mRNA expression and phenotype analysis of single gene repressions in C. glutamicum strains for the double gene repression study. Figure S4. Relative mRNA expression and phenotype analysis of double gene repressions in C. glutamicum strains

MOESM3 of Effective isopropanol–butanol (IB) fermentation with high butanol content using a newly isolated Clostridium sp. A1424

Additional file 3: Table S1. Net NADH balance per one mole of product formation from the corresponding mole of glucose and glycerol

Effect of culture temperature on 2,3-BD production performance by <i>R</i>. <i>ornithinolytica</i> (initial glucose at 50 g/L and pH 6.5).

<p>Effect of culture temperature on 2,3-BD production performance by <i>R</i>. <i>ornithinolytica</i> (initial glucose at 50 g/L and pH 6.5).</p

Fed-batch fermentation of glucose by <i>R</i>. <i>ornithinolytica</i> B6 at various agitation speeds.

<p>pH controlled at 5.5 after the pH reached 5.5 from an initial pH of 7.0 under several agitation speeds (a) 300 rpm, (b) 400 rpm, (c) 500 rpm; ■, glucose; □, growth; ♢, ethanol; ●, acetoin; ○, 2,3-BD; ♦, acetic acid; ▲, succinic acid; △, lactic acid; +, butyric acid.</p

Phylogenetic tree derived from the analysis of the 16S rRNA gene sequences of isolate B6 and related strains.

<p>The tree was inferred using the Neighbor-Joining method. The evolutionary distances were computed using the Maximum Composite Likelihood method. Bootstrap values are from 1000 replications and only those greater than 50% are shown. <i>Serratia marcescens</i> ATCC 13880 was selected as the out group. Evolutionary analyses were conducted in MEGA7. The scale bar indicates 0.2% nucleotide substitution rate.</p

Photosynthetic CO₂ Conversion to Fatty Acid Ethyl Esters (FAEEs) Using Engineered Cyanobacteria

Metabolic engineering of cyanobacteria has received attention as a sustainable strategy to convert carbon dioxide to fatty acid-derived chemicals that are widely used in the food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered for the first time to produce fatty acid ethyl esters (FAEEs) from CO₂. Due to the lack of an endogenous ethanol production pathway and wax ester synthase (AftA) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 by expressing heterologous AftA and introducing the ethanol pathway, resulting in detectable peaks of FAEEs. To enhance FAEE production, a heterologous phosphoketolase pathway was introduced in the FAEE-producing strain to supply acetyl-CoA. Subsequent optimization of the cyanobacterial culture with a hexadecane overlay resulted in engineered S. elongatus PCC 7942 that produced photosynthetic FAEEs (10.0 ± 0.7 mg/L/OD₇₃₀) from CO₂. This paper is the first report of photosynthetic production of FAEEs from CO₂ in cyanobacteria

Production of 2,3-BD and other metabolites using various carbon sources at 25°C (initial carbon source at 50 g/L and pH 7.0).

<p>Production of 2,3-BD and other metabolites using various carbon sources at 25°C (initial carbon source at 50 g/L and pH 7.0).</p

Fed-batch fermentation of glucose by <i>R</i>. <i>ornithinolytica</i> B6 (pBbA5c-<i>budABC</i>); ■, glucose; □, growth; ♢, ethanol; ●, acetoin; ○, 2,3-BD; ♦, acetic acid; ▲, succinic acid; △, lactic acid; +, butyric acid.

<p>Fed-batch fermentation of glucose by <i>R</i>. <i>ornithinolytica</i> B6 (pBbA5c-<i>budABC</i>); ■, glucose; □, growth; ♢, ethanol; ●, acetoin; ○, 2,3-BD; ♦, acetic acid; ▲, succinic acid; △, lactic acid; +, butyric acid.</p