Predictive Value of Plasma MicroRNA-216a/b in the Diagnosis of Esophageal Squamous Cell Carcinoma

Esophageal squamous cell carcinoma (ESCC) is a common human malignancy with poor survival, which was usually diagnosed at an advanced stage. MicroRNAs (miRNAs), a class of single stranded noncoding RNAs with only 17-25 ribonucleotides, were demonstrated to play an important role in lots of cancers. In the recent years, increasing evidence revealed that circulating miRNAs exhibited great potential in the diagnosis of various types of cancers. The present study was designed to evaluate the diagnostic value of plasma miRNA-216a/b for ESCC. Our results showed that the expression level of plasma miRNA-216a/b was significantly lower in ESCC patients compared with that of healthy controls. The receiver operating characteristic (ROC) curve analysis yielded an area under the ROC curve (AUC) value of 0.877 [95% CI (confidence interval): 0.818-0.922] for miRNA-216a and 0.756 (95% CI: 0.685-0.819) for miRNA-216b. Clinical data indicated that plasma miRNA-216a/b were inversely correlated with lymph node metastasis and TNM stage. Additionally, the plasma miRNA-216b expression level was significantly upregulated in postoperative samples compared to preoperative samples. Our study, for the first time, demonstrated that plasma miRNA-216a/b might serve as potential biomarkers for the diagnosis of ESCC and dysregulation of miRNA-216a/b might be involved in the progression of ESCC

Expert Consensus on Microtransplant for Acute Myeloid Leukemia in Elderly Patients -Report From the International Microtransplant Interest Group

Recent studies have shown that microtransplant (MST) could improve outcome of patients with elderly acute myeloid leukemia (EAML). To further standardize the MST therapy and improve outcomes in EAML patients, based on analysis of the literature on MST, especially MST with EAML from January 1st, 2011 to November 30th, 2022, the International Microtransplant Interest Group provides recommendations and considerations for MST in the treatment of EAML. Four major issues related to MST for treating EAML were addressed: therapeutic principle of MST (1), candidates for MST (2), induction chemotherapy regimens (3), and post-remission therapy based on MST (4). Others included donor screening, infusion of donor cells, laboratory examinations, and complications of treatment

The characteristics analysis of strain variation associated with Wenchuan earthquake using principal component analysis

Borehole strainmeters that are installed deeply into bedrock are capable of recording both continuous stress and strain measurements, and have consequently become an important tool for monitoring crustal deformation. A YRY-4 borehole strainmeter installed at the Guza Station recorded anomalous changes in borehole strain data preceding the Wenchuan earthquake on May 12, 2008 (UTC) (=8.0). We apply principal component analysis (PCA) to analyze borehole strain data from the Guza Station. The first principal component eigenvalues and eigenvectors are calculated. The fitted results of the cumulative number of anomalous eigenvalues demonstrate that an acceleration occurred approximately 4 months before the earthquake (from January 2008). The results of the combined eigenvalue and eigenvector analyses show that the spatial distribution of eigenvectors and accelerated occurrence of eigenvalue anomalies represents the stress evolution characteristics of the fault from a steady state to a sub-instability state in rock experiments. We tentatively infer that this process may also be linked to the preparation phase of a large earthquake

Doyne honeycomb retinal dystrophy/malattia leventinese induced by EFEMP1 mutation in a Chinese family

Abstract Background Doyne honeycomb retinal dystrophy (DHRD)/malattia leventinese (ML) is a rare allelic condition with massive drusen in the posterior fundus caused by EFEMP1 gene mutation. Patients showed decreased vision when the lesion affected the macular area. At present, the treatment efficiency is not satisfactory. Case presentation In this study, we presented a family with DHRD/ML disease and analyzed the pathological and genetic information. A 28-year-old female patient presented to our department due to impaired visual acuity for 10 years especially in the right eye with deterioration for 5 months. Gene sequencing was performed by MyGenostics (Peking, China). Gene sequencing results revealed heterozygous mutations in EFEMP1 gene, which were consistent with the DHRD/ ML. Single heterozygous mutation (c.1033C > T) was observed in each of the three blood samples. This missense mutation triggered p.R345W. Conclusions DHRD/ML is a rare disease associated with EFEMP1 gene mutation. Up to now, we are not sure whether these lesions are associated with the onset of DHRD/ML. In future, we hope to find out the exact relationship between them

Theoretical Investigations on Shadow Band Correction Factors for Diffuse Radiation under Isotropic Conditions without Approximation

The precise measurement for irradiance is important for the performance evaluations of solar systems, because both beam and diffuse radiation measurements must be known to calculate the efficiency of a solar system. One of the widely used instruments for diffuse radiation measurement is a pyranometer with a ring. Hence, investigations on shadow band correction factors are necessary. The factors are normally calculated by the expressions of Drummond and Burek et al. under isotropic conditions. However, their calculation results are approximate. While the latitude φ, the inclination angle β and the declination δ are satisfied with the condition of |−tan(φ−β)tanδ|>1, the factors cannot be calculated by their expressions. In this paper, the expressions for the factors under isotropic conditions were obtained by coordinate transformations and discussed in two different coordinate systems without approximation. Our expressions can give precise correction factors in any case and overcome the disadvantages of their expressions. While the sensor is placed horizontally, the maximum relative deviation between the results by the expression in Drummond’s paper and by our expressions is less than 2.3%. When the sensor is placed on a sloped surface, the results by the expressions in Burek’s paper and by our expressions will have considerable differences in some ranges of slope angles and latitudes. The maximum relative deviation is more than 22%

Further Understanding of Degradation Pathways of Microcystin-LR by an Indigenous <i>Sphingopyxis</i> sp. in Environmentally Relevant Pollution Concentrations

Microcystin-LR (MC-LR) is the most widely distributed microcystin (MC) that is hazardous to environmental safety and public health, due to high toxicity. Microbial degradation is regarded as an effective and environment-friendly method to remove it, however, the performance of MC-degrading bacteria in environmentally relevant pollution concentrations of MC-LR and the degradation pathways remain unclear. In this study, one autochthonous bacterium, Sphingopyxis sp. m6 which exhibited high MC-LR degradation ability, was isolated from Lake Taihu, and the degrading characteristics in environmentally relevant pollution concentrations were demonstrated. In addition, degradation products were identified by utilizing the full scan mode of UPLC-MS/MS. The data illustrated that strain m6 could decompose MC-LR (1&#8315;50 &#956;g/L) completely within 4 h. The degradation rates were significantly affected by temperatures, pH and MC-LR concentrations. Moreover, except for the typical degradation products of MC-LR (linearized MC-LR, tetrapeptide, and Adda), there were 8 different products identified, namely, three tripeptides (Adda-Glu-Mdha, Glu-Mdha-Ala, and Leu-MeAsp-Arg), three dipeptides (Glu-Mdha, Mdha-Ala, and MeAsp-Arg) and two amino acids (Leu, and Arg). To our knowledge, this is the first report of Mdha-Ala, MeAsp-Arg, and Leu as MC-LR metabolites. This study expanded microbial degradation pathways of MC-LR, which lays a foundation for exploring degradation mechanisms and eliminating the pollution of microcystins (MCs)

Effects of Microcystin-LR on Metabolic Functions and Structure Succession of Sediment Bacterial Community under Anaerobic Conditions

Microcystins (MCs), which are produced by harmful cyanobacteria blooms, pose a serious threat to environmental health. However, the effect of MCs on the bacterial community under anaerobic conditions is still unclear. This study examined the dynamic changes of MC-degrading capacity, metabolic activity, and structure of the bacterial community in lake sediment repeatedly treated with 1 mg/L microcystin-LR (MC-LR) under anaerobic conditions. The results showed that the MC-degrading capacity of the bacterial community was increased nearly three-fold with increased treatment frequency. However, the metabolic profile behaved in exactly opposite trend, in which the overall carbon metabolic activity was inhibited by repeated toxin addition. Microbial diversity was suppressed by the first addition of MC-LR and then gradually recovered. The 16S amplicon sequencing showed that the dominant genera were changed from Exiguobacterium and Acinetobacter to Prosthecobacter, Dechloromonas, and Agrobacterium. Furthermore, the increase in the relative abundance of Dechloromonas, Pseudomonas, Hydrogenophaga, and Agrobacterium was positively correlated with the MC-LR treatment times. This indicates that they might be responsible for MC degradation under anaerobic conditions. Our findings reveal the relationship between MC-LR and the sediment bacterial community under anaerobic conditions and indicate that anaerobic biodegradation is an effective and promising method to remediate MCs pollution

Theoretical Investigations on Shadow Band Correction Factors for Diffuse Radiation under Isotropic Conditions without Approximation

The precise measurement for irradiance is important for the performance evaluations of solar systems, because both beam and diffuse radiation measurements must be known to calculate the efficiency of a solar system. One of the widely used instruments for diffuse radiation measurement is a pyranometer with a ring. Hence, investigations on shadow band correction factors are necessary. The factors are normally calculated by the expressions of Drummond and Burek et al. under isotropic conditions. However, their calculation results are approximate. While the latitude &phi;, the inclination angle &beta; and the declination &delta; are satisfied with the condition of |&minus;tan(&phi;&minus;&beta;)tan&delta;|&gt;1, the factors cannot be calculated by their expressions. In this paper, the expressions for the factors under isotropic conditions were obtained by coordinate transformations and discussed in two different coordinate systems without approximation. Our expressions can give precise correction factors in any case and overcome the disadvantages of their expressions. While the sensor is placed horizontally, the maximum relative deviation between the results by the expression in Drummond&rsquo;s paper and by our expressions is less than 2.3%. When the sensor is placed on a sloped surface, the results by the expressions in Burek&rsquo;s paper and by our expressions will have considerable differences in some ranges of slope angles and latitudes. The maximum relative deviation is more than 22%