Macro and microscopic evaluation of Gmelina arborea Roxb. – A botanical pharmacognostic approach for quality control of raw drug material

Gmelina arborea Roxb. of family Verbenaceae, is one of the highly valued medicinal plant used in numerous traditional medical formulations. It belongs to the ‘Dasamoola’ group of ayurvedic medicinal plants and hence widely exploited. Pharmacognostic techniques involving macroscopic, microscopic and also dry powder analysis serve as botanical methods which help in the correct identification of the crude drug. Leaf constants such as stomatal number, stomatal index, vein islet number, vein termination number also have been determined. Better quality control practices in nutraceutical and pharmaceutical industries demand the correct identification of the dried plants or powdered drug thereby detects and prevents the adulterations, if any. The challenge ahead of this investigation is to validate the therapeutic efficacy and safety of the plant following standard methodology

Evaluation of anticonvulsant activity of aqueous extract of Adhatoda vasica leaves by MES model in rats

Background: Epilepsy is the commonest neurological condition affecting people of all ages, race and social class. The present study was taken up to evaluate the anticonvulsant effect of aqueous extract of leaves of Adhatoda vasica in rats. To evaluate the effect of aqueous extract of Adhatoda vasica leaves on maximal electroshock model in albino rats and to compare the effect of aqueous extract of Adhatoda vasica leaves with standard dose of Phenytoin on Maximal electro shock model.Methods: Anticonvulsant activity of aqueous extract of Adhatoda vasica was analysed using MES (Maximal electroshock) model. Phenytoin (25mg/kg) as standard for Maximal electroshock, and two doses of aqueous extract of Adhatoda vasica (100mg/kg and 200mg/kg) were used as test drugs. Parameters observed in MES were abolition of hind limb tonic extension (HLTE) and time taken to regain righting reflex.Results: In MES model, control group showed 0% protection and standard phenytoin group showed 100% protection. Aqueous extract of Adhatoda vasica at 100mg/kg and 200mg/kg showed 33.33% and 50% protection from seizures respectively. The mean duration of time to regain righting reflex was significantly reduced in Adhatoda vasica groups when compared to control group (p 0.05) were obtained.Conclusions: Aqueous extract of leaves of Adhatoda vasica has shown significant anticonvulsant action in MES model

PHARMACOLOGY OF NOVAL CANNABINOIDS

Cannabis is a plant rich in various compounds that have a variety of impacts on the physiology of humans and the effects of these metabolites have a significant role in managing a variety of clinical diseases. A substantial increase in the use of SC (synthetic cannabinoids) had seen in the last few years especially infrequent cannabis users. The SCs will generate psychoactive effects that were similar to cannabis. However, the composition and pharmacological characteristics of these drugs make them possibly hazardous. Like all drugs, cannabis’ pharmacokinetics depends on the route of administration. Several studies showed that the bioavailability is less in oral administration when compared to inhalation. The main reason for this decrease in oral bioavailability is that cannabinoids undergo the first-pass metabolism before entering into the systemic circulation whereas in inhalation, it enters the circulation directly through the lungs. Cannabis sativa is a psychoactive plant that contains more than 500 components of which 104 cannabinoids had been identified. Of these, 2 components such as Δ9-THC (Δ9-tetrahydrocannabinol) and CBD (cannabidol) were under the scientific investigation. Δ9-THC is the primary cannabinoid which was responsible for the consequences of psychotrophy. The potency of cannabis is assessed based on the THC concentration of a sample that is the main psychoactive cannabinoid in cannabis. The adverse effects are in direct relation to the concentration of THC in the product after regular cannabis use. It can be assumed that several cannabinoids will find their way into the pharmacies from preclinical research within a century

Anticonvulsant activity of Adhatoda vasica leaves by pentylenetetrazol model: preliminary evaluation in rats

Background: Epilepsy is a disorder characterised by recurrent seizures of cerebral origin with episodes of sensory, motor phenomenon with or without loss of consciousness. The present study was taken up to evaluate the anticonvulsant effect of aqueous extract of leaves of Adhatoda vasica in rats. Objectives of this study is to evaluate the effect of aqueous extract of Adhatoda vasica leaves on Pentylenetetrazol induced seizures in albino rats and to compare the effect of aqueous extract of Adhatoda vasica leaves with standard dose of sodium valproate on Pentylenetetrazol induced seizures in albino rats.Methods: Anticonvulsant activity of aqueous extract of Adhatoda vasica was analysed using PTZ (Pentylenetetrazol) model. Groups used were distilled water as control group, Sodium valproate as standard for Pentylenetetrazol and two doses of aqueous extract of Adhatoda vasica (100mg/kg and 200 mg/kg) for this screening model. Parameters observed for PTZ models were abolition of clonic seizures and time duration between injection of PTZ and onset of seizures.Results: In PTZ model, test group at 200 mg/kg showed 33.33% protection for abolition of clonic seizures, though not comparable to standard group. There was significant increase in the duration of onset of clonic seizures after PTZ injection in both test groups (at 100 mg/kg and 200 mg/kg) when compared to control group.Conclusions: Aqueous extract of leaves of Adhatoda vasica has shown significant anticonvulsant action in PTZ model

Assessment and evaluation of drug information services provided by drug information centre at a multispecialty hospital in Erode, Tamil Nadu, India

Background: To evaluate the performance of Drug Information Centre (DIC) in providing quality drug information services to the health care professionals.Methods: It was a prospective study carried out for a period of six months (June-November 2017). The drug information services provided by the pharmacist at the DIC during ward rounds, direct access, telephone and mail were documented in the drug information request and documentation forms. The quality of drug information provided was assessed with the help of feedback questionnaire which was filled by receivers.Results: A total of 283 queries were received, with a maximum from ward rounds 140(49.47%). On evaluation, it was found that most of the queries were from physicians 207 (73.14%) followed by nurses 33(11.66%), pharmacists 26 (9.18%), patients 17 (6.07%). Among that 178 (62.89%) queries were received from department of general medicine. Most of the queries were intended to update knowledge 110 (38.86%) and for the purpose of better patient care 90 (31.81%). The time frame for an authentic reply ranged from minutes to 24 hours, where a maximum of 110 (38.86%) queries had sent a 24 hours deadline. The majority of the queries were for adverse drug reactions [ADR-60 (21.20%)] and most preferred resources were primary 188 (66.43%).Conclusions: The DIC has always presented and offered itself to be an impressive resource, which is used routinely as an information source by all levels of professionals involved in the health system, in addition to the patients themselves

Early evaluation of cocoa (Theobroma cacao L.) clones as intercrop in coconut gardens under red river region of Assam State, India

A field experiment was conducted at Horticultural Research Station (AICRP on Palms), Kahikuchi of Assam Agricultural University for three consecutive years (2018-20) to study the initial performance of cocoa clones as intercrop in adult coconut (Cocos nucifera L) garden spaced at 7.5 m × 7.5 m taking sixteen cocoa clones. Data on tree height, stem girth, number of pods, pod weight, number of beans pod-1, dry bean weight tree-1 year-1 and single dry bean weight revealed significant variations among the 16 cocoa clones. Dried beans are the prime economic produce of cocoa, and single dry bean weight ranged from 0.95 to 1.4 g. With respect to dry bean yield tree-1, VTLC-20 recorded a significantly higher dry bean yield of 2.2 kg tree-1 followed by VTLC-18 (1.5 kg tree-1) compared to other cocoa clones, and eight clones yielded more than 1.0 kg dry bean tree-1. Results showed that the number of leaves on the crown, number of inflorescences per palm and nut yield of coconut was not negatively affected; rather, there was an increase in the yield of coconut over a period

Melamine formaldehyde-metal organic gel interpenetrating polymer network derived intrinsic Fe-N-doped porous graphitic carbon electrocatalysts for oxygen reduction reaction

Fe, N doped porous graphitic carbon electrocatalyst (Fe-MOG-MF-C), obtained by pyrolysis of an Interpenetrating Polymer Network (IPN) comprised of melamine formaldehyde (MF as hard segment) and Metal-Organic Gel (MOG as soft segment), exhibited significant Oxygen Reduction Reaction (ORR) activity in alkaline medium. BET surface area analysis of Fe-MOG-MF-C showed high surface area (821 m2 g-1), while TEM, Raman and XPS results confirmed Fe and N co-doping. Furthermore, a modulated porous morphology with a higher degree of surface area (950 m2 g-1) has been accomplished for the system (Fe-MOG-MFN-C) when aided by a sublimable porogen, such as naphthalene. XPS results further demonstrated that these systems exhibited a better degree of distribution of graphitic N and an onset potential value of 0.91 V vs. RHE in 0.1 M KOH solution following an efficient four-electron ORR pathway. The electrocatalytic activity of Fe-MOG-MFN-C is superior to that of Fe-MOG-MF-C by virtue of its higher graphitic N content and surface area. Thus, the study presents a new class of IPN derived MF-MOG nanocomposites with the potential to generate extended versions of in situ Fe-N doped porous graphitic carbon structures with superior ORR activity

Defining novel functions for cerebrospinal fluid in ALS pathophysiology

Despite the considerable progress made towards understanding ALS pathophysiology, several key features of ALS remain unexplained, from its aetiology to its epidemiological aspects. The glymphatic system, which has recently been recognised as a major clearance pathway for the brain, has received considerable attention in several neurological conditions, particularly Alzheimer's disease. Its significance in ALS has, however, been little addressed. This perspective article therefore aims to assess the possibility of CSF contribution in ALS by considering various lines of evidence, including the abnormal composition of ALS-CSF, its toxicity and the evidence for impaired CSF dynamics in ALS patients. We also describe a potential role for CSF circulation in determining disease spread as well as the importance of CSF dynamics in ALS neurotherapeutics. We propose that a CSF model could potentially offer additional avenues to explore currently unexplained features of ALS, ultimately leading to new treatment options for people with ALS.</p