An exocyst component, Sec5, is essential for ascospore formation in Bipolaris maydis

In this study, we identified Sec5 in Bipolaris maydis, a homologue of Sec5 in Saccharomyces cerevisiae and a possible exocyst component of the fungus. To examine how Sec5 affects the life cycle of B. maydis, we generated null mutant strains of the gene (Δsec5). The Δsec5 strains showed a strong reduction in hyphal growth and a slight reduction in pathogenicity. In sexual reproduction, they possessed the ability to develop pseudothecia. However, all ascospores were aborted in any of the asci obtained from crosses between Δsec5 and the wild-type. Our cytological study revealed that the abortion was caused by impairments of the post-meiotic stages in ascospore development, where ascospore delimitation and young spore elongation occur

Intergenerational Correlation of Household Wealth : Evidence from the JHPS Second-Generation Supplement

IPSE DP 2021F00

Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection

In a previous study, we reported a transient transformation system using repeated screening for hygromycin B (Hyg) resistance in the basidiomycete Ceriporiopsis subvermispora. In the present study, by combining this technique with CRISPR/Cas9, we demonstrated successful marker-free genome editing in Pleurotus ostreatus, which is one of the most economically important cultivated mushrooms as well as a model white-rot fungus. At first, transformant selection mediated by the transient expression of marker genes was demonstrated using a plasmid harboring the Hyg resistance gene (hph) in P. ostreatus. Then, genome editing of fcy1, which confers 5-fluorocytosine (5-FC) resistance to the host cell, was performed by the transient expression of Cas9, gRNA, and hph and strains with 5-FC resistance and Hyg sensitivity were isolated. Additionally, genome editing of fcy1 in these strains was confirmed by Sanger sequencing. To our knowledge, this is the first report of marker-free genome editing through the transient expression of Cas9, gRNA, and hph in agaricomycetes, which opens the door for repeated genome editing in these fungi

植物病原菌における細胞内タンパク質分解系の研究

京都大学0048新制・課程博士博士(農学)甲第21829号農博第2342号新制||農||1068(附属図書館)学位論文||H31||N5201(農学部図書室)京都大学大学院農学研究科地域環境科学専攻(主査)教授 田中 千尋, 教授 本田 与一, 准教授 刑部 正博学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDGA

Calorimetric and spectroscopic evidence of chain-melting in smectic E and smectic A phases of 4-Alkyl-4′-isothiocyanatobiphenyl (nTCB)

To confirm the molten state of the alkyl chain in soft crystalline phase, smectic E (SmE) phase, thermodynamic and spectroscopic analyses were performed on 4-n-alkyl-4′-isothiocyanatobiphenyl (nTCB, n: the number of carbon atoms in the alkyl group). DSC results of 11TCB and 12TCB, having extra smectic A phase besides smectic E phase, show that their chain-length dependence of entropies of transition ($\Delta _{trs}S$) from the ordered crystalline (OC) phase to the SmE phase matches the trend found for nTCB (n = 4–10), while no chain-length dependence is observed in $\Delta _{trs}S$ at the SmE-to-SmA and SmA-to-isotropic liquid (IL) phase transitions in 11TCB and 12TCB. Temperature dependences of FT-IR spectra of six compounds (n = 2, 3, 5, 8, 10, and 12) were recorded. The CH stretching modes of the chain exhibited more pronounced change at the transition from the OC to the SmE phase than at the transition from the SmE phase to the IL or SmA phase. These results indicate that the alkyl chain is molten in the SmE phase as in IL. The disordering process of nTCB molecules from the OC to IL via anisotropic mesophases is discussed in terms of entropy