The peculiarities of bronchopulmonary system under the influence of adverse ecological factors

This research gives an insight into the problem of atmospheric pollution composition and morbidity that affects the overall well-being and health state of children of Ust-Kamenogorsk. The work shows that age specific features of the respiratory system are characterized by increase in lung volume and bronchial patency trees. The specific methodology assisted to determine the increase in the patency of large and medium bronchi occurs more dynamic in boys than in girls. The bronchial obstruction was assessed by the methods of pneumotachometry with the help of “PneuMedics” (USA) device. The environmental factors of Ust-Kamenogorsk affect the state of the cardiorespiratory system of children, so level of contamination place of residence highly affects the general health state of the children at different age categories and anthropometric measurements. One of the important results was that children with growth and weight coefficient who have higher than the average value are characterized by a decrease of functional reserves of the bronchopulmonary system, and children with the coefficient lower than average, on the contrary, are characterized by decrease in the cardiovascular system

The level of the general reactivity of erythrocytes membranes in rats with combined action of infrasound

This study focuses on the level of the general reactivity of erythrocytes membranes in rats with combined action of infrasound. The effect on biological features was investigated through the experiments on the rats. Comparative analysis of immunological parameters, the permeability of erythrocyte membranes and the electrical conductivity of blood in rats under the effects of infrasound at a frequency of 15 Hz and intensity 11 dB through exposure time of 3-3600 seconds allowed proving that infrasound causes nonspecific changes at the cellular and subcellular levels of the organism. There was used many different technologies and methods. Based on the obtained results, direct indirect and combined effect of infrasound immunological parameters had clearly increased the total count of leukocyte index and all parameters of differential count of leukocytes as well as appearance immature products in the peripheral blood

The study biochemical indicates of blood rats at conditions acute hypoxia

In the experimental simulation hypoxia, the liver and rhinitis coincide with the discontinuation of rheological organs of the bones and organ lymph, which has diminished in shorter duration of the constriction, increasing the viscosity and thrombogenic processes in the blood and lymph. Unilateral hypoxic hypoxia (1.5 hrs) on liver tissue, decreased blood pressure, decreased intoxication levels in beds at 8%. %. The concentration of the total protein concentration and the elevation of the activation ALT and AST were investigated by the changes in the osmotic resistance of the erythrocytes and of urine. It has been established that experimental live animals with hypoxia, lymphatic aching, loss of general protein, high levels of alimentary tract and ALT, AST, abnormalities of rheological properties, and lymph nodes and morphophysiological condition of the lymphatic system

The effect of ecological factors on a cardiovascular system of children

This study provides examination of cardiovascular system of children who live in Ust-Kamenogorsk, mainly in its environmentally polluted areas as industrial zones and Ulbinsk region. The work focused on studying the age and gender characteristics of the cardio vascular system in children living in environmentally unfavorable conditions of Ust-Kamenogorsk. The study highlighted the role of contamination into the development of many diseases that affect cardiovascular system of children in various age categories. The features of cardiorespiratory system were evaluated by methods of pneumotachometry, variational pulsometry, electrocardiography, Holter daily monitoring of electrocardiography. There was detected a gradual increase in sympathetic regulation of heart rate in boys and humoral regulation in girls aged 9-12 years. It was shown that the resistance of children’s organism to the harmful environmental effects depend on their constitutional features. The increased level of stress also affects the well-being of children, as caused by the unfavourable conditions. The method of daily ECG monitoring revealed an increase in the frequency of elevation ST segment, to a lesser extent its depression, so the incidence of bradycardia is more often than tachycardia

Physiologo-morphological features of common wheat under the influence of helium-neon laser

The research work is devoted to researching the comparative influence of electromagnetic radiations with wavelengths on various physiological and morphological parameters of four common wheat Triticum aestivum L. cultivars from Kazakhstan and Egypt. The comprehensive studies concerning various Functional mechanisms of living organisms under the action of electromagnetic radiations is a problem of special priority in biophysics and photobiology, revealing the general and specific plant resistance features to a given radiation source. These are of special importance when electromagnetic irradiations are carried out using various wavelengths, expositions and power intensities. The reactivity responses of living organisms to the action of various physical radiations usually take a part both metabolically and anatomo-morphologically, that is directed mainly to maintaining homeostasis. The purpose was based to reveal the morphological and physiological indicators of seeds and plants of four common wheat Triticum aestivum L. in a comparative aspect before and after the influence of electromagnetic radiations with wavelengths of 400.0 - 800.0 nm. The specific seed reactions feature of Triticum aestivum L have been established after the influence of electromagnetic radiations applying expositions and wavelengths

Reaction of erythrocyte membranes of animals on direct effects of infrasound

This study focuses on the reaction of the membranes of erythrocytes based on the principles that use direct effects of infrasound. The effect on biological features was investigated through the experiments on the rats. The work determined the level of electrical conductivity of blood in rats during direct, indirect and combined effects of infrasound in vivo. There was found that infrasound stimulate the immune system and therefore the results recommend that caution is take place for people who are dealing with these low frequencies. There was used many different technologies and methods, including total differential count in order to consider all of the nuances of the observed object and the main goal. The exposure to infrasound caused increase in the electric conductivity of whole blood than the control in both types of exposure which indicates a large increase of the surface charge density of erythrocyte resulted from the formation of highly reactive species such as superoxide radicals. There were found some undesired biological effects, however further work could have decreased them to a lesser extent

Effects of Almaty city ecological factors on students blood indices

This research gives an insight into the main features of the cardiovascular system’s statistical and dynamic, as well as young local and foreign citizens in Almaty city adaptation indices. 1500 analysis of blood indices was carried out before and after the physical workload. Hematological research were carried out based on N. M. Mykolayiv method and included the erythrocytes and leukocytes count in peripheral blood vessel and absolute number of lymphocytes . Foreign students’ absolute amount of leukocytes and erythrocytes in the peripheral blood vessels and the phagocytic activity of neutrophil were different from local students’ indices

Curcumin and Carnosic Acid Cooperate to Inhibit Proliferation and Alter Mitochondrial Function of Metastatic Prostate Cancer Cells

Anticancer activities of plant polyphenols have been demonstrated in various models of neoplasia. However, evidence obtained in numerous in vitro studies indicates that proliferation arrest and/or killing of cancer cells require quite high micromolar concentrations of polyphenols that are difficult to reach in vivo and can also be (geno)toxic to at least some types of normal cells. The ability of certain polyphenols to synergize with one another at low concentrations can be used as a promising strategy to effectively treat human malignancies. We have recently reported that curcumin and carnosic acid applied at non-cytotoxic concentrations synergistically cooperate to induce massive apoptosis in acute myeloid leukemia cells, but not in normal hematopoietic and non-hematopoietic cells, via sustained cytosolic calcium overload. Here, we show that the two polyphenols can also synergistically suppress the growth of DU145 and PC-3 metastatic prostate cancer cell cultures. However, instead of cell killing, the combined treatment induced a marked inhibition of cell proliferation associated with G0/G1 cell cycle arrest. This was preceded by transient elevation of cytosolic calcium levels and prolonged dissipation of the mitochondrial membrane potential, without generating oxidative stress, and was associated with defective oxidative phosphorylation encompassing mitochondrial dysfunction. The above effects were concomitant with a significant downregulation of mRNA and protein expression of the oncogenic kinase SGK1, the mitochondria-hosted mTOR component. In addition, a moderate decrease in SGK1 phosphorylation at Ser422 was observed in polyphenol-treated cells. The mTOR inhibitor rapamycin produced a similar reduction in SGK1 mRNA and protein levels as well as phosphorylation. Collectively, our findings suggest that the combination of curcumin and carnosic acid at potentially bioavailable concentrations may effectively target different types of cancer cells by distinct modes of action. This and similar combinations merit further exploration as an anticancer modality

Properties of GABAergic Neurons Containing Calcium-Permeable Kainate and AMPA-Receptors

Calcium-permeable kainate and AMPA receptors (CP-KARs and CP-AMPARs), as well as NMDARs, play a pivotal role in plasticity and in regulating neurotransmitter release. Here we visualized in the mature hippocampal neuroglial cultures the neurons expressing CP-AMPARs and CP-KARs. These neurons were visualized by a characteristic fast sustained [Ca2+]i increase in response to the agonist of these receptors, domoic acid (DoA), and a selective agonist of GluK1-containing KARs, ATPA. Neurons from both subpopulations are GABAergic. The subpopulation of neurons expressing CP-AMPARs includes a larger percentage of calbindin-positive neurons (39.4 ± 6.0%) than the subpopulation of neurons expressing CP-KARs (14.2 ± 7.5% of CB+ neurons). In addition, we have shown for the first time that NH4Cl-induced depolarization faster induces an [Ca2+]i elevation in GABAergic neurons expressing CP-KARs and CP-AMPARs than in most glutamatergic neurons. CP-AMPARs antagonist, NASPM, increased the amplitude of the DoA-induced Ca2+ response in GABAergic neurons expressing CP-KARs, indicating that neurons expressing CP-AMPARs innervate GABAergic neurons expressing CP-KARs. We assume that CP-KARs in inhibitory neurons are involved in the mechanism of outstripping GABA release upon hyperexcitation

Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma.

Non-thermal atmospheric pressure plasma has attracted great interest due to its multiple potential biomedical applications with cancer treatment being among the most urgent. To realize the clinical potential of non-thermal plasma, the exact cellular and molecular mechanisms of plasma effects must be understood. This work aimed at studying the prostate cancer specific mechanisms of non-thermal plasma effects on energy metabolism as a central regulator of cell homeostasis and proliferation. It was found that cancer cells with higher metabolic rate initially are more resistant to plasma treated phosphate-buffered saline (PBS) since the respiratory and calcium sensitive signaling systems were not responsive to plasma exposure. However, dramatic decline of cancer oxidative phosphorylation developed over time resulted in significant progression of cell lethality. The normal prostate cells with low metabolic activity immediately responded to plasma treated PBS by suppression of respiratory functions and sustained elevation of cytosolic calcium. However, over time the normal cells start recovering their mitochondria functions, proliferate and restore the cell population. We found that the non-thermal plasma induced increase in intracellular ROS is of primarily non-mitochondrial origin. The discriminate non-thermal plasma effects hold a promise for clinical cancer intervention