Effects of nitrogen source and effective micro-organisms on vegetative growth of shrubs of selected grapefruit (Citrus paradisi Macf) cultivars

   Grapefruit (Citrus Paradisi Macf.) is one of the most important citrus fruits in the Sudan. It can be successfully grown throughout the country where there are suitable soils and sufficient water to sustain tree growth. The objectives of this study were to find out the effects of different nitrogen sources, effective microorganisms (EM) and their combinations on the vegetative growth of shrubs of selected grapefruit cultivars. A field experiment was carried out at Umbarona, Wad Medani, Gezira State, Sudan, latitude 14.38 N, longitude 33.48 E and altitude 409 masl, in June 2012. Treatments consisted of three grapefruit cultivars: Marsh Seedless, Red Blush and Shamber. Fertilizer treatments were urea (100%), chicken manure (100%), urea with chicken manure (50% each), urea with EM, chicken manure with EM, urea (50%)+ chicken manure (50%)+ EM and unfertilized control. All fertilizer treatments were applied at the rate of 43 kg N/ha. Treatments were arranged in a split- plot design with two replicates. Grapefruit cultivars were allotted to the main plots and fertilizer treatments to the sub-plots. Data were collected on plant height (cm), stem diameter (cm), number of new branches, length of new branches (cm) and diameter of new branches (cm). Results showed that Marsh Seedless cultivar had the most vigorous vegetative growth compared to the other two cultivars. The results showed that the combination of urea, chicken manure and EM significantly gave the highest vegetative growth values, followed by chicken manure with EM, urea with chicken manure, urea with EM, urea alone, chicken manure alone and the least values of growth parameters were recorded for the unfertilized control. It is recommended to grow Marsh Seedless grapefruit cultivar and fertilize it with a combination of urea, chicken manure and EM.         يعتبر القريب فروت من أهم ثمار الحمضيات في السودان ويمكن زراعته بنجاح في جميع أنحاء البلاد حيثما توفرت التربة المناسبة والمياه الكافية لنمو الأشجار. أجريت تجربة حقلية في ام بارونة، واد مدنى ولاية الجزيرة، السودان. تهدف هذه التجربة لمعرفة تأثير مصادر النتروجين المختلفة والكائنات الحية الدقيقة الفعالة ومزجها مع بعضها البعض على النمو الخضري لشجيرات أصناف منتخبة من القريب فروت. تضمنت المعاملات ثلاثة أصناف من القريب فروت وهي مارش  ورد بلش وشامبر وسبعة معاملات أسمدة وهي  اليوريا، وزرق الدواجن، يوريا مع زرق الدواجن، يوريا مع الكائنات الحية الدقيقة الفعالة، زرق الدواجن مع الكائنات الحية الدقيقة الفعالة، يوريا مع زرق الدواجن مع الكائنات الحية الدقيقة الفعالة والشاهد. تمت إضافة الأسمدة لكل المعاملات بمعدل 43 كجم نتروجين للهكتار. صممت التجارب بطريقة القطع المنشقة بحيث كانت أصناف القريب فروت هي القطع الرئيسة ومعاملات النتروجين هي القطع الثانوية. جمعت بيانات عن طول النبات (سم) وقطر الساق (سم) وعدد الأفرع الجديدة وطول الفرع (سم) وقطر الفرع (سم). أوضحت النتائج أن الصنف مارش أعطى أعلى نمو خضري مقارنة بالصنفين رد بلش وشامبر. الخليط بين سماد اليوريا وزرق الدواجن و الكائنات الحية الدقيقة الفعالة  أعطت أعلى معدل للنمو الخضري ويليه زرق الدواجن والكائنات الحية الدقيقة الفعالة ثم اليوريا والكائنات الحية الدقيقة الفعالة ثم اليوريا وزرق الدواجن ثم زرق الدواجن لوحده ثم اليوريا لوحده وأدنى معدلات  النمو كانت في الشاهد .  يوصى بزراعة الصنف قريب فروت مارش وتسميده بخليط من اليوريا وزرق الدواجن والكائنات الحية الدقيقة الفعالة

Some characteristics of the Sudanese bread (Kisra) supplement with fenugreek (Trigonella foenum-graecum L)

     In this study the chemical composition of two fenugreek seed types known locally as Sudanese and Ethiopian' types and sorghum kisras supplemented with 5%, 10% and 20% flour of the two fenugreek types after removal of the bitter taste, was determined* The two fenugreek seed types had shown slightly small differences in most of their chemical components, however, the Ethiopian type had slightly more protein (26.4+0.4 %) than that of the Sudanese type (25.5± 0.07 %). On the other hand, the Sudanese type contained more mucilage (23.5±0.04 %) than that of the Ethiopian type (22.6±0.010/0). The most significant effect of kisra supplementation was the increase in good quality protein by a range of 31-66%. It increased from 11.20% in the control kisra to the range of 14.70-18.64%. The crude fibre% of the control kisra (3.40%) increased in the supplemented kisras to the range of 4.54-5.30%. The ash% decreased from 2.42% in the control kisra, to the range of 2.20 - le20% in the supplemented kisras. The sensory analysis indicated that the fenugreek supplemented kisra was accepted by the panelists at the 5% level of fenugreek seed flour supplementation. The overall acceptance level of the different samples decreased with the increase in fenugreek seed flour used in supplementation

Doppler ultrasound of umbilical artery in prediction of fetal outcome in pregnancy induced hypertension Sudanese population

The pregnancy induced hypertension increase the fetal mortality and morbidity and the using of Doppler umbilical artery indices decrease the fetal mortality and morbidity however, there is few complete data about the most frequently altered Doppler US parameters to predict fetal outcome in pregnancy induced hypertension . Methods This ia cohort prospective study done in two hundred and six women of second and third trimester presenting to antenatal clinic in Soba University Hospital at the department of Obstetrics & Gynecology, in the fetus unit and critical pregnancy in the period From June 2008 to April 2013 to assess the Doppler indices of umbilical artery in pregnancy induced hypertension for prediction of prenatal outcome; 105 pregnancy induced hypertension patients and 101 women with uneventful pregnancies as normal control group included in this study . Baseline investigations and color Doppler of umbilical artery were done. Statistical analysis of data were done using SPSS, Receiver Operating Characteristic (ROC) curve analysis was performed and the area under the curve (AUC) used to determine sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of umbilical artery Doppler indices to predict fetal outcome.Results The study determine that there was significant difference in Doppler indices in PIH and control group ( p<0.01, the mean indices of umbilical artery is higher in PIH group compared with normal pregnancy group , the mean different of S/D ratio was 0.40, mean difference of RI was 0.06 and the mean different of PI index was 0.16, high percentage of adverse fetal outcome had been reported in in Pregnancy Induced Hypertension group than in control , which was more in absent and reversed flow velocity in umbilical artery in Pregnancy Induced Hypertension compared with group of Pregnancy Induced Hypertension with present end diastolic flow velocity. Systolic/Diastolic ratio was most accurate in predicting adverse outcome in pregnancy induced hypertension patients, followed by the Pulastility index then the Resistance index (75%, 66% and 57% respectively).ConclusionThis study concluded that pregnancy induced hypertension leads to worsen placental insufficiently, which appears on the higher Doppler indices of umbilical artery to PIH patients when compared with normal pregnancy. A low diastolic flow and higher indices characterized the pregnancies with abnormal outcomes. Doppler of the umbilical artery was useful to predict fetal well being in PIH patients, high percentage of adverse fetal outcome had been reported in absent and reversed end diastolic flow velocity in umbilical artery compared with group of present flow velocity

Production of Polysaccharides (Xanthan gum) by Xanthomonas campestris pv. Sesame

Many microorganisms and plants were found produce polysaccharides which are widely varying in their composition and structure. The extracellular polysaccharide produced by &nbsp;&nbsp;Xanthomonas campestris pv. campestris, is chemically analyzed and used industrially. However, only the xanthan gum which was ranking as the best among the ten polysaccharides was used commercially in large amounts. The xanthan gum has numerous applications in food and other industries. The present study was aiming to investigate the capacity of X; campestris pv. sesami for the production of xanthan gum, using culture media containing different carbon sources. The properties of the produced material were investigated (pH, viscosity, and chromatographic analysis using thin layer chromatography).&nbsp; The results showed that the weight of the product was increasing with time reaching about 9.8g at the tenth day compared to only 2.1g on the second day. The results of the effect of different carbon sources indicated that sucrose was the best giving 9.0 g in the 7th day compared to only 1.5g by raffinose. The pH was changing from 6.7 at the beginning of the experiment to 4.2 in the tenth day. However, the viscosity of the inoculated sucrose medium was found to increase from 2.4 unit at the second day to 9.7 units after ten days. Analysis on paper chromatography showed that the produced polysaccharide contains mannose, glucuronic acid, glucose, rhamnose but no ribose nor trehalose or fructose were detected. The results indicated that the investigated bacterium was producing large amounts of xanthan gum. Although, Xanthan gum was discovered in 1950s its substantial commercial production began in 1964 and in 1969, the USA, Food and Drug Administration authorized its use in food. Xanthan gum, now has numerous uses in food and other industries. Further studies should be carried on other isolates &nbsp;of the bacterium &nbsp;&nbsp;X. campestris pv. sesami and more chemical analysis are needed

Comparative Study Using WEKA for Red Blood Cells Classification

Red blood cells (RBC) are the most common types of blood cells and are the most intensively studied in cell biology. The lack of RBCs is a condition in which the amount of hemoglobin level is lower than normal and is referred to as "anemia". Abnormalities in RBCs will affect the exchange of oxygen. This paper presents a comparative study for various techniques for classifying the RBCs as normal, or abnormal (anemic) using WEKA. WEKA is an open source consists of different machine learning algorithms for data mining applications. The algorithm tested are Radial Basis Function neural network, Support vector machine, and K-Nearest Neighbors algorithm. Two sets of combined features were utilized for classification of blood cells images. The first set, exclusively consist of geometrical features, was used to identify whether the tested blood cell has a spherical shape or non-spherical cells. While the second set, consist mainly of textural features was used to recognize the types of the spherical cells. We have provided an evaluation based on applying these classification methods to our RBCs image dataset which were obtained from Serdang Hospital-alaysia, and measuring the accuracy of test results. The best achieved classification rates are 97%, 98%, and 79% for Support vector machines, Radial Basis Function neural network, and K-Nearest Neighbors algorithm respectively

Air born dust particles effects on microwave propagation in arid-area

Dust storms can degrade visibility and increase atmospheric attenuation. Therefore, microwave (MW) propagation is severely affected by dust storms in many parts of the world. Air-born dust particles may affect electromagnetic waves during a dust storm. In this paper air- born dust particles effects are studied based on measured visibility. Recent analytical and numerical models results are compared to the measured at 14 GHz. Consequently, measured attenuation is significantly greater than the predicted using recent analytical and numerical models. Dust storms can degrade visibility and increase atmospheric attenuation. Therefore, microwave (MW) propagation is severely affected by dust storms in many parts of the world. Air-born dust particles may affect electromagnetic waves during a dust storm. In this paper air- born dust particles effects are studied based on measured visibility. Recent analytical and numerical models results are compared to the measured at 14 GHz. Consequently, measured attenuation is significantly greater than the predicted using recent analytical and numerical models

Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes

Introduction: In recent years, there has been an exponential increase in the number of studies aiming to understand the biology of exosomes, as well as other extracellular vesicles. However, classification of membrane vesicles and the appropriate protocols for their isolation are still under intense discussion and investigation. When isolating vesicles, it is crucial to use systems that are able to separate them, to avoid cross-contamination. Method: EVs released from three different kinds of cell lines: HMC-1, TF-1 and BV-2 were isolated using two centrifugation-based protocols. In protocol 1, apoptotic bodies were collected at 2,000&#x00D7;g, followed by filtering the supernatant through 0.8 &#x00B5;m pores and pelleting of microvesicles at 12,200&#x00D7;g. In protocol 2, apoptotic bodies and microvesicles were collected together at 16,500&#x00D7;g, followed by filtering of the supernatant through 0.2 &#x00B5;m pores and pelleting of exosomes at 120,000&#x00D7;g. Extracellular vesicles were analyzed by transmission electron microscopy, flow cytometry and the RNA profiles were investigated using a Bioanalyzer&#x00AE;. Results: RNA profiles showed that ribosomal RNA was primary detectable in apoptotic bodies and smaller RNAs without prominent ribosomal RNA peaks in exosomes. In contrast, microvesicles contained little or no RNA except for microvesicles collected from TF-1 cell cultures. The different vesicle pellets showed highly different distribution of size, shape and electron density with typical apoptotic body, microvesicle and exosome characteristics when analyzed by transmission electron microscopy. Flow cytometry revealed the presence of CD63 and CD81 in all vesicles investigated, as well as CD9 except in the TF-1-derived vesicles, as these cells do not express CD9. Conclusions: Our results demonstrate that centrifugation-based protocols are simple and fast systems to distinguish subpopulations of extracellular vesicles. Different vesicles show different RNA profiles and morphological characteristics, but they are indistinguishable using CD63-coated beads for flow cytometry analysis

Screening of some sorghum genotypes for resistance to sorghum midge, Stenodiplosis (= Contarinia) sorghicola Coqillet (diptera: Cecidomyiidae) under gedarif rainfed conditions

Preliminary experiments were conducted at Northern area, Gedarif State during seasons of 2002-03, 2006-07.Where as advanced trials during 2008/2009 to 2012/2013 at northern and Southern areas. The objective was to evaluate selected sorghum genotypes for sorghum midge resistance. A total of 3000 accessions were obtained from Gene Bank Resources. Resistant genotype, DJ 6514 (Resistant Check already released in 2007) was obtained from International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). Result showed that the midge damage rating was significantly different among genotypes. The midge damage rating scores ranged between, 1.3- 8.6; 1.1- 9.2; 1.4- 9.0; 1.1 – 9.4; and 1.2- 9.2 for all seasons (2008/09; 2009/10; 2010/11; 2011/12 and 2012/13). However, the lowest midge damage rating was recorded by DJ 6514 (Resistant check), followed by P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Wad Baco; and Safra (1.4; 1.5; 1.5, 1.6 and 1.7), respectively. Genotypes, P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Wad Baco; Safra and Harerai showed lowest % yield loss and performed similar to the resistant check (14.2; 14.5; 14.5; 17.0 and 17.3%), respectively. The combined analysis showed that a significant difference was observed between genotypes. The genotypes were significantly different in panicle types, compact and semi-compact headed genotypes showed lower % glumes coverage (1.3- 4.5%), while semi-compact headed genotypes ranged between 5.5 – 7.8%. Genotypes, Wad Baco; P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Safra; Wad Ahmed; Harerai and Wad Akar showed the shortest glumes coverage (1.3; 1.3; 1.5; 1.5; 1.6; 1.7 and 1.8 %). Compact headed genotypes recorded lowest midge density (6.5 adults/ 5 heads), while the semi-compact headed genotypes were recorded 15.7 adults/ 5 heads compared with others types of heads

Differential Regulation of the PGC Family of Genes in a Mouse Model of Staphylococcus aureus Sepsis

The PGC family of transcriptional co-activators (PGC-1α [Ppargc1a], PGC-1β [Ppargc1b], and PRC [Pprc]) coordinates the upregulation of mitochondrial biogenesis, and Ppargc1a is known to be activated in response to mitochondrial damage in sepsis. Therefore, we postulated that the PGC family is regulated by the innate immune system. We investigated whether mitochondrial biogenesis and PGC gene expression are disrupted in an established model of Staphylococcus aureus sepsis both in mice with impaired innate immune function (TLR2−/− and TLR4−/−) and in wild-type controls. We found an early up-regulation of Ppargc1a and Ppargc1b post-infection (at 6 h) in WT mice, but the expression of both genes was concordantly dysregulated in TLR2−/− mice (no increase at 6 h) and in TLR4−/− mice (amplified at 6 h). However, the third family member, PRC, was regulated differently, and its expression increased significantly at 24 h in all three mouse strains (WT, TLR2−/−, and TLR4−/−). In silico analyses showed that Ppargc1a and Ppargc1b share binding sites for microRNA mmu-mir-202-3p. Thus, miRNA-mediated post-transcriptional mRNA degradation could account for the failure to increase the expression of both genes in TLR2−/− mice. The expression of mmu-mir-202-3p was measured by real-time PCR and found to be significantly increased in TLR2−/− but not in WT or TLR4−/− mice. In addition, it was found that mir-202-3p functionally decreases Ppargc1a mRNA in vitro. Thus, both innate immune signaling through the TLRs and mir-202-3p-mediated mRNA degradation are implicated in the co-regulation of Ppargc1a and Ppargc1b during inflammation. Moreover, the identification of mir-202-3p as a potential factor for Ppargc1a and Ppargc1b repression in acute inflammation may open new avenues for mitochondrial research and, potentially, therapy

First products of DNA marker-assisted selection in sorghum released for cultivation by farmers in sub-saharan Africa

Striga hermonthica (Del.) Benth. is the major biotic constraint to sorghum production. Its control is difficult and can only be achieved through integrated management strategies that depend mainly on host plant resistance and enhanced soil fertility. However, breeding for resistance is hampered by the complexity of host parasite interactions and lack of reliable screening methods. The invention of molecular markers has enhanced the effectiveness of breeding for resistance. Five genomic regions (QTLs) with linked markers associated with Striga resistance were mapped in sorghum variety N13 by [10]. In this study, to increase the efficiency of marker-assisted selection (MAS), 27 EST-SSR markers in close association with Striga resistance QTLs were also identified and mapped. Populations of backcross (BC3S4) derived from N13 (Striga resistant) X three farmer preferred sorghum cultivars: Tabat, Wad Ahmed and AG-8 (Striga susceptible) were generated. Thirty-one lines (BC3S4) with confirmed Striga field resistance were genotyped with foreground and background selection makers. Twenty resistant lines, with two or more major QTLs were selected for regional evaluation. Of these 10 lines were selected and advanced for multi-location testing, together with Wad Ahmed, Tabat, AG-8, N13, SRN39 and IS9830 as checks. Standard variety trials were conducted in Striga sick plots over three seasons (2009-2011) in Sudan, Gezira Research Station, Damazine, Sinnar, and Gedarif. Results revealed that four lines (T1BC3S4, AG6BC3S4, AG2BC3S4 and W2BC3S4) were Striga resistant and agronomically superior with yields ranging from 180% to 298% higher relative to their recurrent parents. This Striga resistance coupled with superior attributes of the recurrent parent (including very high yield potentials, high grain quality and drought tolerance) will provide adaptation and stability across a wide range of environments. These are the first products of DNA markerassisted selection (MAS) in sorghum released for cultivation by farmers in sub-Saharan Africa