Polimorfizam CYP11B1 i PPARGC1A gena i njegov utjecaj na kontrolu reproduktivnih obilježja te procjenu uzgojne vrijednosti za proizvodnju mlijeka u prvoj laktaciji Bos indicus (deoni) goveda

In the present study, in Deoni, the dual purpose cattle breed of Southern India, maintained in a National Dairy Research Institute herd, Southern Regional Station, Bengaluru, molecular characterization of the putative exon 1 of CYP11B1 and putative intron 9 and 3’UTR of PPARGC1A genes was carried out in 146 animals using the PCR-RFLP technique. Three restriction enzymes, namely PstI, HaeIII and NheI, were used for digestion of the amplicons of the genes, respectively. In the putative exon 1 of the CYP11B1 gene, two genotypes, VV and VA, were detected with frequencies of 0.23 and 0.77, respectively. The frequencies of allele V and A in the population were found to be 0.62 and 0.38, respectively. The allelic frequencies of C and T types were observed as 0.63 and 0.37, with frequencies of CC, TC and TT genotypes as 0.38, 0.51 and 0.11 in the putative intron 9 of the PPARGC1A gene, respectively. Three genotypes, namely AA, AC and CC were detected in 3’UTR of the PPARGC1A gene, with respective frequencies of 0.75, 0.21 and 0.04. The allelic frequencies of A and C types were 0.86 and 0.14, respectively. The locus (c.1892+19T>C) in the putative intron 9 of the PPARGC1A gene was found to be significant (PC) genes. No significant (PC) u pretpostavljenom intronu 9 PPARGC1A gena utvrđen je statistički znakovit (PC). U istraženih goveda nije opažena znakovita (P<0,10) povezanost između lokusa gena i uzgojne vrijednosti za proizvodnju mlijeka u prvoj laktaciji