19 research outputs found
Combination of HPLCâorbitrapâMS/MS and network pharmacology to identify the antiâinflammatory phytochemicals in the coffee leaf extracts
Abstract In this study, we investigated the phytochemical compositions and the associated antiâinflammatory activity of coffee leaf fractions prepared by sequential solvent extraction using highâperformance liquid chromatographyâorbitrapâtandem mass spectrometry (HPLCâorbitrapâMS/MS) combined with network pharmacology. The results showed that the ethyl acetate fraction (EACâL) had the highest nitric oxide (NO), ABTS, and DPPH free radical scavenging abilities due to the higher concentrations of mangiferin, rutin, 3,5âdicaffeoylquinic acid (3,5âdiCQA), and 4,5âdiCQA. The extraction solvents had the greatest impact on the antiâinflammatory activity of coffee leaf fractions, whereas the processing method had the most significant effect on the antioxidant activity of these fractions. Untargeted metabolomics analysis using HPLCâorbitrapâMS/MS indicated that palmitic acid, 3,4âdihydroxybenzaldehyde, and caffeic acid may be involved in the antiâinflammatory activity of EACâL fraction obtained from fresh coffee leaves. On the other hand, processed coffee leaf fraction exhibited antiâinflammatory activity that was attributed to the presence of 9S,13Râ12âoxophytodienoic acid, pinocembrin, and quercetin, which have high degree values associated with the inflammation network. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment of network pharmacology analysis showed that these 35 differential compounds in the coffee leaf fractions affect cell transcription, apoptosis, phosphorylation, NO synthesis, phosphatidylinositide 3âkinasesâprotein kinase B (PI3KâAkt) signaling pathway, focal adhesion, hypoxiaâinducible factorâ1, hepatitis, cancer, and so on. This result indicated that coffee leaf extract may also function as an inhibitor for inflammationârelated cancers. The findings of our research are valuable in guiding the extraction of antiâinflammatory components from coffee leaves
Structural Approximate Reanalysis for Topological Modifications of Finite Element Systems
Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage via PI3K/Akt/eNOS Pathway
Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage via PI3K/Akt/eNOS Pathway
Danggui Sini decoction (DSD) is a traditional Chinese decoction, which is wildly applied and showed to be effective in ameliorating ischemia-related symptoms. However, the mechanisms of DSD action in ischemic damage remain to be fully clarified. Pancreatic islet endothelial cells are pivotal constituent of islet microvasculature, with high vulnerability to hypoxic injuries. Here, using MST1 cell, a pancreatic islet endothelial cell-line, as a model, we investigated the effects of DSD on hypoxia-stimulated endothelial cell lesions and its underlying mechanisms. We found that DSD-Containing Serum (DSD-CS), collected from DSD-treated rats, could efficiently protect MST1 survival and proliferation from Cobalt chloride (CoCl2) induced damage, including cell viability, proliferation, and tube formation. Furthermore, DSD-CS restored the activity of PI3K/Akt/eNOS signaling inhibited by CoCl2 in MST1 cells. The protective effect of DSD-CS could be blocked by the specific PI3K/Akt/eNOS inhibitor LY294002, suggesting that DSD-CS protection of MST1 cell survival from hypoxia was mediated by PI3K/Akt/eNOS pathway. In conclusion, DSD treatment protected MST1 survival from hypoxic injuries via PI3K/Akt/eNOS pathway, indicating its role in protecting microvascular endothelial cells