IDENTIFIKASI SENYAWA BIOAKTIF PADA KULIT IKAN SEMBILANG (Paraplotosus albilabris) DAN POTENSINYA SEBAGAI ANTIBAKTERI

Eel tail catfish is one of the aquatic organisms that contains bioactive compounds. These bioactive compounds have the potential to be developed into anticancer, antimicrobial, and antioxidant properties. The aim of this study was to extract bioactive compounds and measure antibacterial activity. Maceration using three different organic solvents (ethanol, n-hexane, and methanol) was used to remove the bioactive compounds on fish skin. Analysis indicators carried out were identification of bioactive compound, toxicity assay (Brine Sirmp Lethaly Test), and antibacterial activity (agar diffusion). The results of this study showed that ethanol and methanol were able to remove more bioactive compounds than n-hexane, the LC50 of fish skin extract from eel tail catfish (ethanol, methanol, and n-hexane) which was 451.42 ppm; 33853.22 ppm; 29513.84 ppm. The extraction with ethanol had the highest inhibition zone 93 mm for Staphylococcus aureus and 74 mm for Escherichia coli bacteria. It is suspected that the bioactive is classified as a polar bioactive compound.    Eel tail catfish is one of the aquatic organisms that contains bioactive compounds. These bioactive compounds have the potential to be developed into anticancer, antimicrobial, and antioxidant properties. The aim of this study was to extract bioactive compounds and measure antibacterial activity. Maceration using three different organic solvents (ethanol, n-hexane, and methanol) was used to remove the bioactive compounds on fish skin. Analysis indicators carried out were identification of bioactive compound, toxicity assay (Brine Sirmp Lethaly Test), and antibacterial activity (agar diffusion). The results of this study showed that ethanol and methanol were able to remove more bioactive compounds than n-hexane, the LC50 of fish skin extract from eel tail catfish (ethanol, methanol, and n-hexane) which was 451.42 ppm; 33853.22 ppm; 29513.84 ppm. The extraction with ethanol had the highest inhibition zone 93 mm for Staphylococcus aureus and 74 mm for Escherichia coli bacteria. It is suspected that the bioactive is classified as a polar bioactive compound.   &nbsp

Pengaruh Lama Presto dan Konsentrasi Natrium Bikarbonat (NaHCO3) terhadap Karakteristik Tepung Tulang Ikan Tenggiri (Scomberomorus commerson)

Most fish processing industries only use fish meat as raw material for industry. Whereas fish bones are contain high mineral content needed by the human body and can be utilized as high calcium fishbone meal. Fishbone meal in this reaseach was made from waste from the fishery industries in Tanjungpinang, Riau Islands. Mackerel fishbone meal was made in several steps consist of preparation of raw materials, boiling of fishbones, washing and cleaning of the bones, presto processing of fish bones with the addition of sodium bicarbonate (NaHCO3), drying, siege and sieving. The purpose of this study is to determine the effect of presto duration and concentration of NaHCO3. This research was conducted by the factorial randomized block design method (RAKF) with two factors: duration of presto (2 hours (P1) and 4 hours (P2)) and concentration of NaHCO3 (0% (N1), 1% (N2) and 2% (N3)). The results showed that the duration of presto treatment and the concentration of sodium bicarbonate (NaHCO3) had a significant effect on the 5% level of yield but not significant on calsium content. The best results showed on 2 hours presto with addition NaHCO3 1%. The best treatment was used as a sample to measure the chemical characteristics of mackerel fish bone meal. Fish bone meal obtained 4.86% water content, 69.84% ash content, 3.72% protein content, 17.21% fat content, 12.56% phosphorus content, and 0.46% crude fiber. The chemical content of fishbone meal has been appropriate with Indonesian Nasional Standard

Komposisi Kimia Hidrolisat Protein Jeroan Ikan dengan Konsep Autolisis Menggunakan Enzim Internal pada Ikan

Penelitian ini bertujuan untuk menganalisis komposisi asam amino yang terkandung pada hidrolisat protein jerona ikan yang di autolisis menggunakan enzim internal pada ikan. Hidrolisis protein jeroan ikan optimum terjadi pada suhu 60°C dengan waktu 4 jam. Hasil analisis menunjukkan bahwa kadar protein hidrolisat protein jeroan ikan sebesar 54,36%. Hidrolisat jeroan ikan yang diautolisis menggunakan enzim internal memiliki kandungan sebesar 18,12%.  Proses defatting sangat dibutuhkan untuk menurunkan kandungan lemak pada bahan baku sehingga proses hidrolisis bisa berjalan dengan baik. Hidrolisat protein jeroan ikan mengandung beberapa jenis asam amino seperti histidin, treonin, prolin, tirosin,leusin, asam aspartat, lisin, glisin, argini, alanin, valin, isoleusin, fenilalanin, asam glutamat dan serin. Kadar asam amino yang paling tinggi adalah asam glutamat yang diikuti oleh leusin. Asam amino yang paling rendah adalah tirosi

PEMANFAATAN TERIPANG PASIR (Holothuria scabra) SEBAGAI MINUMAN FUNGSIONAL

Research on the use of sea cucumber (Holothuria scabra) is a functional drink has been carried out. The purpose of this study was to find out the proximate analysis of fresh sea cucumber meat, to determine the best formulation of sea cucumber functional drinks, find out the antioxidative activity and identification of saponin from functional drinks. The best formulation of sand cucumber (H. scabra)functional drinks based on organoleptic tests is MFT1 sea cucumber functional drinks(20% seacucumber, 20% lemongrass, 20% sugar, 20% rosella flower, 10% citrus, and10% ginger). The functional drink has positive saponins. The antioxidant activity contained in these functional drinks is very weak because the IC50 value is greater than 200 ppm which is 1838 ppm.Penelitian tentang pemanfaatan teripang pasir (Holothuria scabra) sebagai minuman fungsional telah dilakukan bertujuan untuk mengetahui analisis proksimat daging teripang segar, untuk mendapatkan formulasi terbaik dari minuman fungsional teripang pasir, mengetahui analisis antioksidan dan analisis saponin dari minuman fungsional. Formulasi minuman fungsional teripang pasir (H. scabra)terbaik berdasarkan uji organoleptik adalah minuman fungsional teripang MFT1 (20% teripang, 20% serai, 20% gula, 20% bunga rosella, 10% jeruk, dan 10% jahe). Minuman fungsional tersebut positif mengandung saponin. Aktivitas antioksidan yang terdapat dalam minuman fungsional tersebut adalahsangat lemah, karena nilai IC50-nya lebih besar dari 200 ppm yaitu 1838ppm

EFEK PENAMBAHAN EKSTRAK RUMPUT LAUT Eucheuma Cottonii PADA PEMBUATAN ES BATU TERHADAP KEMUNDURAN MUTU KERANG LOKAN (Geloina erosa) DAN KERANG DARAH (Anadara granosa)

Phylum molusca is a group of animals that are soft-bodied and have no spine (invertebrates). One of the phylum molusca is a class of Bivalvia that is generally in the form of lateral symmetry, a type of bivalvia that is often found in the water of Riau Islands is Lokan shells (Geloina erosa) and Darah shells (Anadara granosa) that live in tidal areas. The purpose of this study is to find out if the addition of water ice cubes resulting from the extraction of seaweed Echeuma Cottonii is effective for lokan shells and blood shells. Based on the results of the study obtained for the meat rendment of each shell is 85.39% in lokan shells and 84.27% in darah shells. Organoleptic results showed a more effective treatment of shellfish treatment with the addition of regular ice cubes, Water Holding Capacity (WHC) for the treatment of water ice cube extract binding or absorbing more water than regular ice cube treatment. The second pH of shellfish from three treatments had a value below 7, the lowest acidic pH obtained treatment with seaweed ice cubes

UJI TOKSISITAS EKSTRAK DAUN BERUWAS LAUT (Scaevola taccada) DENGAN METODE BRINE SHRIMP LETHALITY TEST(BSLT)

Beach Naupaka leaves (Scaevola taccada) originating from the coast of Tanjung Siambang, Riau Islands are thought to have secondary metabolites. This study aims to determine the level of toxicity and active compounds in young leaves and old leaves with marine characteristics. Extraction of active compounds is done in two ways, namely extraction without using solvents to produce crude extracts and extraction using chlorofrom solvents to produce crude extracts of chloroform. Each extract was tested for toxicity against Artemia salina Leach shrimp larvae. The results showed that the toxicity of crude extracts from beach naupaka leaves against shrimp larvae of Artemia salina Leach obtained LC50 values, namely crude extracts of young leaves and old leaves of beach naupaka amounted to 6006.20 ppm and 59841.16 ppm. Whereas crude chloroform extracts of young and old beach naupaka leaves are 410204.10 ppm and 7153.19 ppm. Phytochemical test results show that crude extract and crude extract of chloroform are steroids and reducing sugars. This result was reinforced by TLC using the best eluent, for crude extracts young leaves of eluent chloroform  produced 7 spots with an Rf value of 0.088-0.213. Crude extract old leaves of eluent chloroform produced 7 spots with an Rf value of 0.169-0.263. Crude extract of chloroform young leaves of eluent metanol:chloroform (1:1) produced 10 spots with an Rf value of 0.025-0.275. And crude chloroform extract old leaves of eluent chloroform  produced 11 spots with an Rf value of 0.006-0.281.Daun beruwas laut (Scaevola taccada) yang berasal dari pantai Tanjung Siambang, Kepulauan Riau diduga memiliki senyawa-senyawa metabolit sekunder. Senyawa-senyawa tersebut kemungkinan merupakan senyawa bioaktif yang dapat digunakan dalam dunia farmakologi. Penelitian ini bertujuan untuk mengetahui tingkat toksisitas dan senyawa aktif pada daun muda dan daun tua beruwas laut. Ekstraksi senyawa aktif dilakukan dengan dua cara yaitu ekstraksi tanpa menggunakan pelarut menghasilkan ekstrak kasar dan ekstraksi menggunakan pelarut klorofrom menghasilkan ekstrak kasar kloroform. Masing-masing ekstrak diuji toksisitasnya terhadap larva udang Artemia salinaLeach. Hasil penelitian menunjukkan bahwa hasil uji toksisitas ekstrak kasar daun beruwas laut terhadap larva udang Artemia salinaLeach diperoleh nilai LC50 yaitu ekstrak kasar daun muda dan daun tua beruwas laut sebesar 6006,20 ppm dan 59841,16 ppm. Sedangkan ekstrak kasar kloroform daun muda dan daun tua beruwas laut sebesar 410204,10 ppm dan 7153,19 ppm. Hasil uji fitokimia menunjukkan bahawa ekstrak kasar dan ekstrak kasar kloroform adalah steroid dan gula pereduksi. Hasil ini diperkuat dengan KLT menggunakan eluen terbaik, untuk ekstrak kasar daun muda uluen kloroform menghasilkan 7 spot dengan nilai Rf 0,088-0,213. Ekstrak kasar daun tua uluen kloroform menghasilkan 7 spot dengan nilai Rf 0,169-0,263. Ekstrak kasar kloroform daun muda uluen metanol:kloroform (1:1) menghasilkan 10 spot dengan nilai Rf 0,025-0,275. Dan Ekstrak kasar kloroform daun tua uluen kloroform menghasilkan 11 spot dengan nilai Rf 0,006-0,281

PENGARUH PEREBUSAN TERHADAP KARAKTERISTIK ASAM AMINO DAN LOGAM BERAT PADA DAGING KEONG BAKAU (Telescopium telescopium)

Amino acids are important constituents of proteins in the body. Heavy metal is a metal element with a density of more than 5 g / cm3. this study aims to determine the yield of mangrove snail (Telescopium telescopium), proximate composition, characteristics of amino acids and heavy metals (Pb, Cd and Hg) fresh and boiled mangrove snails from Tanjung Unggat Coastal Tanjungpinang City. The study began with sampling mangrove snails, morphometric measurements, body yield (shells, meat and innards) and analysis of chemical content, amino acids and heavy metals in mangrove snail meat. The results of the proximate research, the highest value was found in fresh mangrove snail water content which was equal to 78.58%. The highest amino acid content in mangrove snails was found in glutamic acid at 25.121 Heavy metals Pb, Cd and Hg were not detected in mangrove snail meat.Asam amino merupakan unsur penting penyusunprotein dalam tubuh. Logam berat adalah unsur logam dengan massa jenis lebih dari 5 g/cm3 . Tujuan penelitian ini adalah untuk menentukan rendemen keong bakau (Telescopium telescopium), komposisi proksimat, karakteristik asam amino dan logam berat (Pb, Cddan Hg) keong bakau segar dan rebus dari perairan Tanjung Unggat Kota Tanjungpinang. Penelitian ini diawali dengan pengambilan sampel keong bakau, pengukuran morfometrik, rendemen (cangkang, daging dan jeroan), analisis kandungan kimia, asam amino dan logam berat pada daging keong bakau. Berdasarkan hasil penelitian proksimat nilai tertinggi terdapat pada kadar air keong bakau yaitu sebesar 78,58%. Kandungan asam amino tertinggi pada keong bakau terdapat pada asam glutamat sebesar 25,121 Kandungan logam berat Pb, Cd dan Hg tidak terdeteksi pada daging keong bakau

ANALISIS KANDUNGAN VITAMIN DAN MINERAL DARI BUAH BERUWAS LAUT(Scaevola taccada)

The purpose of this study was to determine the content of vitamins, minerals, proximate, and food fibres from Beruwas Laut (Scaevola taccada). This research was conducted descriptively. The results of the research analyzed concluded that the Beruwas Laut (Scaevola taccada) was water content (80.994 %), ash content (0.318 %), lipid content (0.573 %), protein content (0.312 %), and carbohydrate (17.803 %) while the vitamin content in the Beruwas Laut (Scaevola taccada) namely vitamin A (12.415 SI), vitamin B12 (0.025 SI), vitamin E (0.130 SI), vitamin C (22.578 SI) and mineral content in the Beruwas Laut (Scaevola taccada) namely Magnesium (4.326 %), Iron (1.321 %), Calcium (28.775 %), Potassium (116.228 %), Phosphorus (8.519 %), Iodine (13.733 %). Based on the results of the study it can be concluded that the analysis of the content of vitamins and minerals in the Beruwas Laut (Scaevola taccada) contains vitamin C and mineral content of Potassium, the highest research results.  Tujuan penelitian ini untuk mengetahui kandungan vitamin, mineral, proksimat, dan serat pangan dari buah beruwas laut(Scaevola taccada).Penelitian ini dilakukan secara deskriptif. Hasil penelitian yang telah dianalisis menyimpulkan bahwa pada buah beruwas laut(Scaevola taccada) yaitu kadar air (80.994 %), kadar abu (0.318 %), kadar lemak (0.573 %), kadar protein(0.312 %), dan karbohidrat (17.803 %) sedangkan kandungan vitamin pada buah beruwas laut(Scaevola taccada) yaitu vitamin A (12.415SI), vitamin B12 (0.02mg), vitamin E (0.130mg), vitamin C (22.578 mg) dan kandungan mineral pada buah beruwas laut (Scaevola taccada) yaitu magnesium (4.326 mg), besi (1.321 mg), kalsium (28.775 mg), kalium (116.228 mg), fosfor (8.519 mg), iodium (13.733 mg). Berdasarkan hasil penelitian dapat disimpulkan analisis kandungan vitamin dan mineral pada buah beruwas laut(Scaevola taccada) terdapat kandungan vitamin C dan kandungan mineral Kalium yang hasil penelitiannya paling tinggi

Characterization of Jelly Candy from Seaweed (Gelidium sp.) and Gelatin

Jelly candy was formulated with additional Gelidium and gelatin. Gelidium and gelatin contain components that can improve the texture. The objective of this study was to know the chemical quality from Gelidium sp. jelly formulation. Three treatments GA (content of Gelidium was 63,2%), GB (content of Gelidium was 61,80%) and GC (content of Gelidium was 60,4%) were analyzed to see their chemical quality. The highest moisture content was obtained at GA treatment (19,13%). The highest ash content was obtained at GC treatment (0,45%). The highest sugar content obtained at GB treatment (55,22%). The highest Protein content obtained at GC treatment (21,89%). The lead (Pb) unidentified in a product after formulation

SOSIALISASI PENGOLAHAN MINUMAN FUNGSIONAL DARI TERIPANG DENGAN MENERAPKAN TEKNOLOGI THERMAL DI KAMPUNG MADUNG KELURAHAN KAMPUNG BUGIS KOTA TANJUNGPINANG PROVINSI KEPULAUAN RIAU

Kepulauan Riau merupakan salah satu Provinsi yang mengedepankan sektor perikanan sebagai salah satu ujung tombak perekonomiannya. Industri pada sektor tersebut masih didominasi oleh industri kecil menengah (IKM) yang umumnya bergerak pada produk olahan hasil perikanan. UMRAH sebagai salah satu Universitas di Provinsi Kepulauan Riau memiliki kewajiban dalam membantu mengembangkan industri kecil menengah (IKM) tersebut dengan kegiatan pengabdian kepada masyarakat. Adapun mitra yang dipilih merupakan kelompok masyarakat pengolah produk perikanan yang berada di sekitar kampus FIKP UMRAH. Pada dasarnya program ini merupakan bentuk kegiatan solutif dari pihak kampus kepada mitra sebagai masyarakat sasaran. Adapun permasalahan utama pada mitra yaitu masih kurangnya pengetahuan tentang diversifikasi produk pangan dari pengolahan teripang sehingga hanya dijual dalam bentuk kering, padahal hal ini sangat berguna untuk meningkatkan nilai ekonomis dari produk. Solusi utama adalah sosialisasi pengolahan minuman fungsional teripang dengan penerapan teknologi thermal pada kemasannya sehingga menghasilkan produk yang bernilai ekonomis dan lebih awet dalam penyimpanan. Metode program ini dibagi menjadi beberapa tahapan yaitu penentuan kader, diskusi atau tanya jawab kepada masyarakat sasaran yang akan memberikan kesempatan kepada MItra untuk melakukan proses pemahaman materi atau menyampaikan gagasan dan permasalahan yang terkait dengan diversifikasi produk olahan teripang,  sosialisasi praktik pengolahan minuman fungsional dari teripang, pembagian buku saku sebagai pengetahuan dasar tentang penganekaragaman produk olahan hasil perikanan. Tujuan pengabdian ini adalah meningkatkan pengetahuan tentang penganekaragaman produk hasil perikanan dari teripang dengan menggunakan teknologi thermal pada kemasannya. Hasil pengabdian yang ingin dicapai adalah khalayak sasaran dapat mengolah teripang menjadi minuman fungsional dan menerapkannya pada industri/kelompokny