RR Lyrae stars in the inner LMC: Where did they form?

RR Lyrae stars (RRLS) belong to population II and are generally used as a tracer of the host galaxy halo. The surface as well as vertical distribution of RRLS in the inner Large Magellanic Cloud (LMC) are studied to understand whether these stars are actually formed in the halo. RRLS identified by the OGLE III survey are used to estimate their number density distribution. The scale-height of their distribution is estimated using extinction corrected average magnitudes of ab type stars. The density distribution mimics the bar, confirming results in the literature. The distribution of their scale height indicates that there may be two populations, one with smaller scale-height, very similar to the red clump stars and the other, much larger. The distribution of the reddening-corrected magnitude along the minor axis shows variation, suggesting an inclination. The inclination is estimated to be i = 31.3 (3.5) degrees, very similar to the inclination of the disk. Thus, the RRLS in the inner LMC mimic the bar and inclination of the disk, suggesting that a major fraction of RRLS is formed in the disk of the LMC. The results indicate that the RRLS in the inner LMC trace the disk and probably the inner halo. They do not trace the extended metal-poor halo of the LMC. We suggest that a major star formation event happened in the LMC at 10-12 Gyrs ago, resulting in the formation of most of the inner RRLS, as well as probably the globular clusters, inner halo and the disk of the LMC.Comment: A&A Letters (in press

Disk of the Small Magellanic Cloud as traced by Cepheids

The structure and evolution of the disk of the Small Magellanic Cloud (SMC) are traced by studying the Cepheids. We aim to estimate the orientation measurements of the disk, such as the inclination and the position angle of the line of nodes, and the depth of the disk. We used the V and I band photometric data of the fundamental and first-overtone Cepheids from the Optical Gravitational Lensing Experiment survey. The period-luminosity relations were used to estimate the relative distance and reddening of each Cepheid. A weighted least-square plane fitting method was then applied to estimate the structural parameters. The line-of-sight depth and then the orientation corrected depth or thickness of the disk were estimated from the relative distance measurements. The period-age-colour relation of Cepheids were used to derive the age of the Cepheids. A break in the PL relations of the fundamental-mode and first-overtone Cepheids at P ~ 2.95 days and P ~ 1 day are observed. An inclination of 64$^o$.4$\pm$0$^o$.7 and a PA$_{lon}$=155$^o$.3$\pm$6$^o$.3 are obtained from the full sample. A reddening map of the SMC disk is also presented. The orientation-corrected depth or thickness of the SMC disk is found to be 1.76 $\pm$ 0.6 kpc. The scale height is estimated to be 0.82 $\pm$ 0.3 kpc. The age distribution of Cepheids matches the SMC cluster age distribution. The radial variation of the disk parameters mildly indicate structures/disturbances in the inner SMC (0.5 $<$ r $<$ 2.5 degree). Some of the Cepheids found in front of the fitted plane in the eastern regions are possibly the youngest tidally stripped counterpart of the H {{\sc i}} gas of the Magellanic Bridge. The Cepheids behind the fitted plane are most likely the population in the Counter Bridge predicted in recent numerical simulations. Different scenarios for the origin of the extra-planar Cepheids are also discussed.Comment: Accepted for publication in the journal Astronomy & Astrophysics, 18 pages, 18 figure

Structure of the Large Magellanic Cloud from the Near Infrared magnitudes of Red Clump stars

The structural parameters, like the inclination, i and the position angle of the line of nodes (PA_lon) of the disk of the Large Magellanic Cloud (LMC) are estimated using the JH photometric data of red clump stars from the Infrared Survey Facility - Magellanic Cloud Point Source Catalog (IRSF-MCPSC). The observed LMC region is divided into several sub-regions and stars in each region are cross identified with the optically identified red clump stars to obtain the near infrared magnitudes. The peak values of H magnitude and (J-H) colour of the observed red clump distribution are obtained by fitting a profile to the distributions and also by taking the average value of magnitude and colour of the red clump stars in the bin with largest number. Then the dereddened peak H0 magnitude of the red clump stars in each sub-region is obtained. The RA, Dec and relative distance from the center of each sub-region are converted into x, y & z Cartesian coordinates. A weighted least square plane fitting method is applied to this x,y,z data to estimate the structural parameters of the LMC disk. A reddening map based on (J-H) colour of the RC stars is presented. When the peaks of the red clump distribution were identified by averaging, an inclination of 25.7 +/- 1.6 and PA_lon = 141.5 +/- 4.5 were obtained. We estimate a distance modulus of 18.47 +/- 0.1 mag to the LMC. Extra-planar features which are in front as well as behind the fitted plane are identified which match with the optically identified extra-planar features. The bar of the LMC is found to be part of the disk within 500 pc. The estimates of the structural parameters are found to be independent of the photometric bands used for the analysis. We find that the inner disk, within 3.0, is less inclined and has larger value of PA_lon when compared to the outer disk.Comment: 13 pages, 16 figures, Accepted for publication in the journal Astronomy & Astrophysic

Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis

<p>Abstract</p> <p>Background</p> <p>Obg is a highly conserved GTP-binding protein that has homologues in bacteria, archaea and eukaryotes. In bacteria, Obg proteins are essential for growth, and they participate in spore formation, stress adaptation, ribosome assembly and chromosomal partitioning. This study was undertaken to investigate the biochemical and physiological characteristics of Obg in <it>Mycobacterium tuberculosis</it>, which causes tuberculosis in humans.</p> <p>Results</p> <p>We overexpressed <it>M. tuberculosis </it>Obg in <it>Escherichia coli </it>and then purified the protein. This protein binds to, hydrolyzes and is phosphorylated with GTP. An anti-Obg antiserum, raised against the purified Obg, detects a 55 kDa protein in immunoblots of <it>M. tuberculosis </it>extracts. Immunoblotting also discloses that cultured <it>M. tuberculosis </it>cells contain increased amounts of Obg in the late log phase and in the stationary phase. Obg is also associated with ribosomes in <it>M</it>. <it>tuberculosis</it>, and it is distributed to all three ribosomal fractions (30 S, 50 S and 70 S). Finally, yeast two-hybrid analysis reveals that Obg interacts with the stress protein UsfX, indicating that <it>M. tuberculosis </it>Obg, like other bacterial Obgs, is a stress related protein.</p> <p>Conclusions</p> <p>Although its GTP-hydrolyzing and phosphorylating activities resemble those of other bacterial Obg homologues, <it>M. tuberculosis </it>Obg differs from them in these respects: (a) preferential association with the bacterial membrane; (b) association with all three ribosomal subunits, and (c) binding to the stress protein UsfX, rather than to RelA. Generation of mutant alleles of Obg of <it>M. tuberculosis</it>, and their characterization in vivo, may provide additional insights regarding its role in this important human pathogen.</p

Pulsating stars in the VMC survey

The VISTA survey of the Magellanic Clouds system (VMC) began observations in 2009 and since then, it has collected multi-epoch data at Ks and in addition multi-band data in Y and J for a wide range of stellar populations across the Magellanic system. Among them are pulsating variable stars: Cepheids, RR Lyrae, and asymptotic giant branch stars that represent useful tracers of the host system geometry.Comment: 8 pages, 7 figures, proceeding contribution of invited presentation at "Wide-field variability surveys: a 21st-century perspective", San Pedro de Atacama (Chile