1 research outputs found
PKC epsilon facilitates recovery of exocytosis after an exhausting stimulation
It has been well documented that protein kinase Cs (PKCs) play multifaceted roles in regulating exocytosis of neurotransmitters and hormones. But the isoform-specific PKC effects are still poorly elucidated mainly because of the large variety of PKC isoforms and the dubious specificity of the commonly used
pharmacological agents. In the present study, based on
overexpression of wild-type or dominant negative PKCĪµ, we demonstrate in neuroendocrine PC12 cells that PKCĪµ, but not PKCĪ±, facilitates recovery of exocytosis after an exhausting stimulation. Specifically, PKCĪµ mediates fast recovery of the extent of exocytosis in a phosphatidylinositol biphosphate-dependent manner, likely through enhancing the rate of vesicle delivery and reorganization of cortical actin network. In addition,
PKCĪµ promotes fast recovery of vesicle release kinetics
that is slowed after a strong stimulation. These experimental results may suggest a PKC-dependent mechanism relevant to the short-term plasticity of exocytosis in both neurons and neuroendocrine cells.Accepted versio