A comprehensive study on the role of the Yersinia pestis virulence markers in an animal model of pneumonic plague

We determined the role of Yersinia pestis virulence markers in an animal model of pneumonic plague. Eleven strains of Y. pestis were characterized using PCR assays to detect the presence of known virulence genes both encoded by the three plasmids as well as chromosomal markers. The virulence of all Y. pestis strains was compared in a mouse model for pneumonic plague. The presence of all known virulence genes correlated completely with virulence in the Balb/c mouse model. Strains which lacked HmsF initially exhibited visible signs of disease whereas all other strains (except wild-type strains) did not exhibit any disease signs. Forty-eight hours post-infection, mice which had received HmsF– strains regained body mass and were able to control infection; those infected with strains possessing a full complement of virulence genes suffered from fatal disease. The bacterial loads observed in the lung and other tissues reflected the observed clinical signs as did the cytokine changes measured in these animals. We can conclude that all known virulence genes are required for the establishment of pneumonic plague in mammalian animal models, the role of HmsF being of particular importance in disease progression

United States Geological Survey Report 213

From abstract: The Bulloch group of uranium claims are in T. 40 S., R. 9 we, Kane County, Utah. The past production has been 8.5 tons of submarginal ore submitted to the Marysvale purchasing depot of the Atomic Energy Commission in 1950; this shipment assayed 0.16 percent U308, Uranium is finely disseminated in clay, carbonaceous clay, carbonized wood fragments, iron-oxide concretions, petrified logs, sandstone, and conglomerates of the lower part of"the Dakota sandstone and upper part of the Summerville formation. Small quantities of carnotite, autunite, and torbernite are exposed in the conglomerate and sandstone on Lynn Nos. 2 and 3 claims

Tiger Eye No. 1 uranium prospect, Sevier County, Utah /

Missing Figure 1, Index map, Tiger Eye No. 1 claim, Sevier County, Utah; and Figure 2, Uranium prospect, Tiger Eye No. 1 claim, Sevier County, Utah ."December 1950."Submitted to U.S. Atomic Energy Commission, Raw Materials Operations.Mode of access: Internet