131 research outputs found
Examining the Relationship Between Clinical Judgment and Nursing Actions in Prelicensure Students
Faculty frequently use the Lasater Clinical Judgment Rubric (LCJR) to identify students’ clinical judgment; however, it is unclear whether LCJR scores relate to appropriate nursing action. In a pilot study consisting of senior-level nursing students (N = 22), participants were scored on the LCJR and anticipated nursing actions, and results were analyzed using simple linear regression. There was a statistically significant correlation (r = 0.364) between clinical judgment and nursing action. Results suggest that total LCJR scores may be an indicator of the completion of indicated nursing action
Development of Imaging Protocols for Neural Progenitor Cell Xenotransplantation
Adult hippocampal progenitor cells (AHPCs) can become three different cell types: neurons, oligodendrocytes, and astrocytes. The goal of this study is to determine whether external or internal factors are more prevalent in regulating cell differentiation, and if AHPCs can differentiate into alternative cell types under appropriate conditions. The AHPCs were transplanted into zebrafish blastulas. The blastula is a permissive environment for transplanted cells to grow and differentiate. The embryos were whole-mount imaged after immunohistochemistry to determine cell localization and type. The embryos were also sectioned to acquire more detailed images. A novel protocol was designed in which test embryos were immunolabeled, whole-mount imaged, and cryosectioned at three time points. The labeling was maintained in the vasculature in in both whole and sectioned embryos. An in vitro experiment was performed to observe if AHPCs maintain homogeneity over time, ensuring that the same cell type was transplanted in each experiment. Immunocytochemistry at the same three time points demonstrated that AHPCs maintain their progenitor cell identity in culture. The techniques established here will be used to determine the factors affecting cell differentiation. The manipulation of these factors to produce desired cell phenotypes may then be used to promote regeneration and repair
A Capsule of Some Unique Events in the History of the Iowa State University Veterinary College
A timeline of notable events in the history of the College of Veterinary Medicine
Exploring the Pursuit of Doctoral Education by Nurses Seeking or Intending to Stay in Faculty Roles
The purpose of this study was to describe the factors influencing the pursuit and completion of doctoral education by nurses intending to seek or retain faculty roles. Traditionally, doctoral education evolved to focus on the preparation of nurses to conduct scientific research, primarily through the doctor of philosophy programs. Most recently, the doctor of nursing practice degree emerged and was designed for advanced practice nurses to be clinical leaders who translate research into practice and policy. Nurses who pursue doctoral education in order to assume or maintain faculty roles must choose between these degrees if they desire a doctorate within the discipline; however, factors influencing their decisions and the intended outcomes of their choice are not clear. During this study, 548 nurses (current students or recent graduates of doctoral programs) completed a comprehensive survey to generate critical evidence about the factors influencing the choices made. Principal findings are related to the issues of time, money, and program selection. These findings can be used to develop strategies to increase enrollment and, therefore, the number of doctorally prepared faculty who are specifically prepared to excel as nursing faculty
Development of Imaging Protocols for Neural Progenitor Cell Xenotransplantation
Adult hippocampal progenitor cells (AHPCs) can become three different cell types: neurons, oligodendrocytes, and astrocytes. The goal of this study is to determine whether external or internal factors are more prevalent in regulating cell differentiation, and if AHPCs can differentiate into alternative cell types under appropriate conditions. The AHPCs were transplanted into zebrafish blastulas. The blastula is a permissive environment for transplanted cells to grow and differentiate. The embryos were whole-mount imaged after immunohistochemistry to determine cell localization and type. The embryos were also sectioned to acquire more detailed images. A novel protocol was designed in which test embryos were immunolabeled, whole-mount imaged, and cryosectioned at three time points. The labeling was maintained in the vasculature in in both whole and sectioned embryos. An in vitro experiment was performed to observe if AHPCs maintain homogeneity over time, ensuring that the same cell type was transplanted in each experiment. Immunocytochemistry at the same three time points demonstrated that AHPCs maintain their progenitor cell identity in culture. The techniques established here will be used to determine the factors affecting cell differentiation. The manipulation of these factors to produce desired cell phenotypes may then be used to promote regeneration and repair.</p
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