A Rapid Flexible Method for Determining Bile Lipids

A rapid flexible method has been developed for the quantitative determination of bile lipids In gallbladder and hepatic bile and duodenal aspirates. Quantification of bile salts involves separation of bile salt conjugates from one another and other bile lipids by thin layer chromatography. The separated salts are determined using 3-hydroxysteroid dehydrogenase and gas liquid chromatography. Cholesterol is determined in petroleum ether extracts of saponified bile by application of the Lieberman-Burchard reaction. Phospholipid phosphorus is determined in purified bile lipid extracts by oxidation followed by application of Bartlett\u27s modification of the Fiske- SubbaRow method

Effects of Tetracycline on Fecal Bile Acid Pool Composition in a Human: A Preliminary Report

We studied the effect of tetracycline on bile acid pool composition in a human subject. A dosage of 250 mg (qid) of tetracycline for one week completely eliminated deoxycholic and lithocholic acid from the pool. Tetracycline may be of value for limiting the build-up of lithocholic acid in patients who are treated with chenodeoxycholic acid to dissolve gallstones