Upstream-binding factor is sequestered into herpes simplex virus type 1 replication compartments

Previous reports have shown that adenovirus recruits nucleolar protein upstream-binding factor (UBF) into adenovirus DNA replication centres. Here, we report that despite having a different mode of viral DNA replication, herpes simplex virus type 1 (HSV-1) also recruits UBF into viral DNA replication centres. Moreover, as with adenovirus, enhanced green fluorescent protein-tagged fusion proteins of UBF inhibit viral DNA replication. We propose that UBF is recruited to the replication compartments to aid replication of HSV-1 DNA. In addition, this is a further example of the role of nucleolar components in viral life cycle

DATA REPORT

INTRODUCTION Ocean Drilling Program (ODP) Leg 116 cored the distal part of the Bengal Fan at three closely spaced sites (717-719). The recovered sediments consisted dominantly of turbidites that varied in thickness between a few centimeters and 2 m or more. A number of different facies have been identified in the sequence and are described by Stow et al. (this volume). Representative examples of these facies types were selected and sampled for grain-size analysis. The results of these analyses are tabulated in this data report. METHODS Sediment samples of approximately 2-g dry weight were placed in 50-cm 3 beakers and disaggregated in a solution of 10% Calgon. To assist in complete disaggregation of the sediment, an ultrasonic probe was used for approximately 2 min. on each sample. Suspended sample concentrations were of the order of 20 g/L. The prepared suspensions were stored in 150-cm 3 plastic bottles. The grain-size analyses were performed by laser diffraction using Malvern particle sizers, models 35OOD and 2600C. The principles of particle-size analysis by laser diffraction are described by The Malvern laser-sizers consist of a laser source, beam expander, sample chamber, focusing lens, ring detector, and a microcomputer. In this study each analysis used a 100-mm focal length focusing lens yielding data consisting of 15 size classes between 1.9 and 188 µm. A percentage of sample outside of this range above and below these limits is also given. The grain-size distribution was computed using the model-independent program option. The sample suspension is introduced into a small ultrasonic tank from which the suspension is continuously pumped through the sample chamber in the path of the laser. The main attraction of laser diffraction analysis for this study was the relatively small sample size required for analysis. Only 2-5 cm 3 of the prepared suspensions are required for each analysis. Several repeat analyses can therefore be performed even on very small original samples. An additional advantage is the speed with which analyses can be performed, generally about 10 min per sample. For many of the samples, duplicate or triplicate analyses were performed to test the reproducibility of the results. 1 Cochran, J. R., Stow, D.A.V., et al., 1990. Proc. ODP, Sci. Results, 116: College Station, TX (Ocean Drilling Program)

29. CONSOLIDATION CHARACTERISTICS AND PERMEABILITY OF BENGAL FAN SEDIMENTS DRILLED DURING LEG 116

ABSTRACT The state of consolidation of outer Bengal Fan deposits is closely related to their depositional history. Early Pleistocene sediments are underconsolidated, i.e., fabric strength is lower than under equilibrium with the present overburden stress, at depths greater than 60-80 mbsf. This may be due to rapid accumulation (> 15-20 cm/k.y.) and overlying low-permeability (< 10~7 cm/s) deposits. The underlying Pliocene sediments are overconsolidated, i.e., fabric strength is higher than in equilibrium with the present overburden stress; at Sites 717 and 719 the sediments are slightly overconsolidated, whereas at Site 718 the overconsolidation is significant. Overconsolidation is explained by erosion that occurred during the early Pleistocene for which a stratigraphic gap was recorded in the drilled cores. The eroded section is estimated to be in the range of 10-40 m at Sites 717 and 719, respectively, and 130-150 m at Site 718. Below 250-300 mbsf the most sediments are normally consolidated. The amount of erosion seems to be related to block rotation and uplift due to intraplate deformation

Drop Splashing on a Dry Smooth Surface

The corona splash due to the impact of a liquid drop on a smooth dry substrate is investigated with high speed photography. A striking phenomenon is observed: splashing can be completely suppressed by decreasing the pressure of the surrounding gas. The threshold pressure where a splash first occurs is measured as a function of the impact velocity and found to scale with the molecular weight of the gas and the viscosity of the liquid. Both experimental scaling relations support a model in which compressible effects in the gas are responsible for splashing in liquid solid impacts.Comment: 11 pages, 4 figure

The Cadiz Contourite Channel: Sandy contourites, bedforms and dynamic current interaction

The Cadiz Contourite Channel is the largest and most prominent contourite channel in the middle slope of the Gulf of Cadiz, and is known to channelise the southern branch of the Lower Core of Mediterranean Outflow Water (MOW) as it flows westwards from the Gibraltar Gateway. The channel lies in water depth between 650 and 1500 m, is 150 km long, 2–12 km wide, up to 120 m deep, and broadly s-shaped in plan view. It has several associated subparallel marginal channels and shorter spillover channel segments. Its geometry is controlled by the interaction of a strong bottom current with the seafloor morphology, affected by neotectonic deformation and diapiric intrusion. Bottom photographs and dredge hauls reveal a channel floor shaped by high-energy flow, in places with bare rock, boulders and gravel, and elsewhere covered with sandy contourites. The rocky substrate and derived clasts are formed of authigenic iron-rich carbonates, testifying the high degree of fluid escape from adjacent diapiric ridges and mud volcanoes. The sandy substrate shows a wide range of current-induced bedforms including small, straight-crested ripples, large sinuous sand waves and dunes (wavelength 3.5–5 m, height 0.3–0.9 m), weak surface lineation on sands, and aligned gravel stringers and deep erosive scours around large boulders. Bedform orientation indicates flows directed to the south/south-west (main channel) and west (spillover channel), which can be related to MOW bottom currents, and current velocities that vary between about 0.2 and 0.8 m s− 1, even in the same channel location. However, current vane orientation was clearly responding, at least in part, to tidal effects and periodicity in the Gulf of Cadiz at the time the photographs were taken. Maximum current velocities are achieved by a combination of barotropic and internal tides (probably generated at the continental slope) that reinforce the normal MOW flow. In addition, meteorologically-induced internal waves with periods shorter than tidal ones may exert an even greater influence on current intensity, especially when they occur at times of sudden changes of meteorological forcing. This effect further influences MOW variability. In all cases, the funnelling effect of the Cadiz Channel amplifies tidal or meteorologically-induced bottom currents

Selfsimilar solutions in a sector for a quasilinear parabolic equation

We study a two-point free boundary problem in a sector for a quasilinear parabolic equation. The boundary conditions are assumed to be spatially and temporally "self-similar" in a special way. We prove the existence, uniqueness and asymptotic stability of an expanding solution which is self-similar at discrete times. We also study the existence and uniqueness of a shrinking solution which is self-similar at discrete times.Comment: 23 page

Macrosystems ecology: Understanding ecological patterns and processes at continental scales

Macrosystems ecology is the study of diverse ecological phenomena at the scale of regions to continents and their interactions with phenomena at other scales. This emerging subdiscipline addresses ecological questions and environmental problems at these broad scales. Here, we describe this new field, show how it relates to modern ecological study, and highlight opportunities that stem from taking a macrosystems perspective. We present a hierarchical framework for investigating macrosystems at any level of ecological organization and in relation to broader and finer scales. Building on well-established theory and concepts from other subdisciplines of ecology, we identify feedbacks, linkages among distant regions, and interactions that cross scales of space and time as the most likely sources of unexpected and novel behaviors in macrosystems. We present three examples that highlight the importance of this multiscaled systems perspective for understanding the ecology of regions to continents

Evolution of the gulf of Cadiz margin and southwest Portugal contourite depositional system : Tectonic, sedimentary and paleoceanographic implications from IODP expedition 339

Acknowledgments This research used samples and data collected through the Integrated Ocean Drilling Program (IODP). The research was partially supported through the CTM 2008-06399-C04/MAR, CTM 2012-39599-C03, CGL2011-26493, CTM2012-38248, SA263U14, IGCP-619, INQUA 1204 and FWF P25831-N29 Projects. Some data were collected with 94-1090-C03-03 (FADO) and MAR-98-0209 (TASYO) Projects. Research was conducted in the framework of the Continental Margins Research Group of the Royal Holloway University of London, People and the Program (Marie Curie Actions) of the European Union's Seventh Framework Program FP7/2007-2013/ under REA Grant Agreement No. 290201 MEDGATE’. We are very grateful to REPSeOL, TGS–NOPEC, and the CSIC-Institut Jaume Almera (http://geodb.ictja.csic.es) for allowing us to use an unpublished seismic data from the Gulf of Cadiz. We thank J. Aguire (UGR, Spain) for comments and suggestions concerning the Pliocene and Quaternary outcrops, B. van den Berg (USAL) for organizing a thought-provoking field-trip to Cadiz, Spain in November, 2014, M. Ángel Caja, L. García Diego, and J. Tritlla (REPSOL) for provenance and diagenetic analysis of early Pliocene sandstones and debrites, and L.J. Lourens (Utrecht University) for providing us the eccentricity and 200-Kys glacio-eustatic sea-level curves included in the Figure 16. Both Prof. D.A.V. Stow (Heriot-Watt Univ., UK) and F.J. Hernández-Molina (RHUL, UK), as the main co-proponents of the IODP Proposal 644 and the co-chiefs of the IODP Exp. 339, thanks to IODP, Exp. IODP 339 Scientists; JR crew and technicians, as well as all people, institutions and companies involved in making IODP a success since 2003. Finally, we also thank the editor, Gert J. De Lange and the reviewers T. Mulder (Bourdeaux Univ.); D. Van Rooij (Ghent Univ) and J. Duarte (Monash Univ.) for their very positive and helpful feedback and discussions in publishing this research.Peer reviewedPublisher PD