2 research outputs found
Rejuvenation solution as an adjunct cold storage solution maintains physiological haemoglobin oxygen affinity during earlyâstorage period of red blood cells
BackgroundRed blood cell (RBC) units accumulate morphologic and metabolic lesions during storage before transfusion. Pyruvateâinosineâphosphateâadenine (PIPA) solutions (Rejuvesol, Biomet, Warsaw, IN) can be incubated with RBC units to mitigate storage lesions. This study proposes a PIPA treatment process, termed cold ârejuvenationâ, using Rejuvesol as an adjunct additive solution, to prevent biomechanical storage lesions while avoiding the 1 h PIPA incubation required with standard PIPA treatment. We compared the efficacy of cold to standard ârejuvenationâ in improving metabolic lesions that occur during cold storage of RBCs, without altering function.MethodsTwelve leucoreduced, Aâpositive RBC units were obtained. Each unit was aliquoted into either control (standard storage), washed (W), standard rejuvenation (SR) or cold rejuvenation (CR) groups, the latter two requiring washing. A volumeâadjusted dose of Rejuvesol was instilled into the CR group upon receipt (Day 3). After 15 days of storage, p50, RBC deformability, inâbag haemolysis and mechanical fragility were analysed. âAny treatmentâ is defined as W, SR and CR, with comparisons in reference to control.ResultsHigher p50s were seen in rejuvenated groups (>30 mmHg vs. <19 mmHg; P < 0·0001). Any treatment significantly increased elongation index (P = 0·034) but did not significantly increase inâbag haemolysis (P = 0·062). Mechanical fragility was not significantly different between groups (P = 0·055) at baseline, but the control (CTL) group was more fragile after 2 h in a cardiac bypass simulation than any treatment (P < 0·0001).ConclusionsThis study demonstrates that rejuvenation (standard or cold) prevents the leftward p50 shift of storage lesions without detrimental effect on RBC deformity, inâbag haemolysis or mechanical fragility.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/156162/2/vox12910_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/156162/1/vox12910.pd
Rejuvenation solution as an adjunct cold storage solution maintains physiological haemoglobin oxygen affinity during earlyâstorage period of red blood cells
BackgroundRed blood cell (RBC) units accumulate morphologic and metabolic lesions during storage before transfusion. Pyruvateâinosineâphosphateâadenine (PIPA) solutions (Rejuvesol, Biomet, Warsaw, IN) can be incubated with RBC units to mitigate storage lesions. This study proposes a PIPA treatment process, termed cold ârejuvenationâ, using Rejuvesol as an adjunct additive solution, to prevent biomechanical storage lesions while avoiding the 1 h PIPA incubation required with standard PIPA treatment. We compared the efficacy of cold to standard ârejuvenationâ in improving metabolic lesions that occur during cold storage of RBCs, without altering function.MethodsTwelve leucoreduced, Aâpositive RBC units were obtained. Each unit was aliquoted into either control (standard storage), washed (W), standard rejuvenation (SR) or cold rejuvenation (CR) groups, the latter two requiring washing. A volumeâadjusted dose of Rejuvesol was instilled into the CR group upon receipt (Day 3). After 15 days of storage, p50, RBC deformability, inâbag haemolysis and mechanical fragility were analysed. âAny treatmentâ is defined as W, SR and CR, with comparisons in reference to control.ResultsHigher p50s were seen in rejuvenated groups (>30 mmHg vs. <19 mmHg; P < 0·0001). Any treatment significantly increased elongation index (P = 0·034) but did not significantly increase inâbag haemolysis (P = 0·062). Mechanical fragility was not significantly different between groups (P = 0·055) at baseline, but the control (CTL) group was more fragile after 2 h in a cardiac bypass simulation than any treatment (P < 0·0001).ConclusionsThis study demonstrates that rejuvenation (standard or cold) prevents the leftward p50 shift of storage lesions without detrimental effect on RBC deformity, inâbag haemolysis or mechanical fragility.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/156162/2/vox12910_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/156162/1/vox12910.pd