Konvektivno sušenje parčića ploda batata (Ipomoea batatas (L.) Lam), zavisnost od predtretmana

The dried of pieces of sweet potatoes (Ipomoea batatas (L.) Lam) was carried in experimental convective cabinet drier with loess. This vegetable was rich in beta carotene and was not sufficiently in our countries. The dried process was carried at 55oC, and weight changes were measured every 15 minutes. Were performed three pretreatments: dried with pretreatment, blanched with the 1% vitamin C and 5% NaCl and without pretreatment. Blanched was carried in water at 60oC, for 3 minutes. The moisture content of raw sweet potato was 76.40%, but in dried material value from 16.90 to 19.59%, respectively. Rehydration values were measure of the quality of the dried process. The best results were obtained to dried small pieces of sweet potato without pretreatment, than rehydration was 83.37%.Sušenje parčića ploda batata (Ipomoea batatas (L.) Lam) ili slatkog krompira vršeno je u eksperimentalnoj konvektivnoj sušari sa lesama. Ova povrtarska biljka bogata je beta karotenom, a nedovoljno se koristi u našoj zemlji. Proces sušenja odvijao se na 55oC, a merena je promena mase na svakih 15 minuta. Rađena su tri predtretmana blanširanjem: bez dodataka (predtretman I), sa dodatkom 1% vitamina C (predtretman II), sa dodatkom 5% NaCl (predtretman III) i sušenje bez predtretmana (kontrola). Blanširanje je vršeno u destilovanoj vodi temperature 60oC za 3 minuta, sa i bez dodataka. Sadržaj vlage u sirovom plodu batatu bio je 76,40%, a u osušenom materijalu kretao se od 16,90 do 19,59 %. Stepen rehidratacije korišćen je kao mera kvaliteta procesa sušenja. Najbolji rezultati dobijeni su kod sušenja parčića ploda batata bez predtretmana, čiji je stepen rehidratacije bio 83,37%

Uticaj klimatskih uslova područja na prinos pšenice

The aim of this study was to analyze the influence of climatic conditions in the city of Leskovac, in the period from 2000 to 2008th year, the yield of wheat. This paper presents the average yield of wheat according to the mean monthly temperature and total precipitation during the vegetation period. So they separated two years ago (2003) when he achieved the lowest yield of wheat in the territory of Leskovac (2.4 t ha-1) and the 2004th years ago, when achieved the highest average yield in the period (3.7 t ha- 1). The average yield in the year with favorable schedule and rainfall, and average air temperature was higher by 1.3 t ha-1 of the unfavorable climatic conditions. Deficiency, poor distribution of rainfall, high average temperature, and temperature fluctuations during the growing negative impact on wheat yield. By choosing a variety that is more tolerant to drought, as well as quality and timely application of agro-technical measures can mitigate the adverse impact of climate conditions on the yield of wheat fields.Cilj ovih istraživanja je bio da se analizira uticaj klimatskih uslova na području grada Leskovca, u periodu od 2000.-2008. godine, na prinos pšenice. U radu su prikazani prosečni prinosi pšenice u zavisnosti od srednjih mesečnih temperatura i ukupnih padavina u periodu vegetacije pšenice. Tako su izdvojene dve godine (2003), kada je ostvaren najniži prinos pšenice na teritoriji Leskovca (2.4 t ha-1) i 2004. godina, kada je ostvaren najviši prosečan prinos u posmatranom periodu (3.7 t ha-1). Prosečan prinos u godini sa povoljnijim rasporedom i količinom padavina, kao i srednjom temperaturom vazduha bio je veći za 1.3 t ha-1 od godine sa nepovoljnim klimatskim prilikama. Nedostatak, loš raspored padavina, visoke prosečne temperature, i temperaturna kolebanja u toku vegetacije negativno utiču na prinos pšenice. Izborom sorte koja je tolerantnija prema suši, kao i kvalitetnom i pravovremenom primenom agrotehničkih mera može se ublažiti nepovoljan uticaj klimatskih prilika područja na prinos pšenice

SIGNIFICANCE OF THERAPY LENGTH WITH NORFLOXACIN TREATMENT OF LOWER URINARY TRACT INFECTION

The aim of the paper was to examine the significance of the therapy with Norfloxacin as mono therapy in the treatment of the lower urinary tract infection and to examine the effectiveness of this antibiotic nowadays as it has not been used for a long time (it was not available on our market).Eighty patients with the lower urinary tract infection were examined. Patients were divided into two groups according to the length of therapy and the age of patients. Before and after the therapy, some examinations were done: clinical check – up, urinary analysis (general check – up), KSL, SE, urea, creatinin, urinoculture, urinary tract EHO.There was no statistically significant difference in the number of patients with the lower urinary tract infections after 3 and 7 days of therapy with Norfloxacin. Often, the cause of this infection was Escherichia coli. The highest frequency of this infection was reported in the aged and female population. There was no statistically significant difference in the number of the patients with the lower urinary tract infection after the therapy with Norfloxacin in relation to the age.There were no differences in the effects of the medicine between the group whose therapy lasted for 3 days and the group whose therapy lasted for 7 days. There was no difference in the effects of the medicine without regard to the age of the patients, too

Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue

We present herein a case report style article on a rare advanced triple-negative breast cancer (TNBC) patient with 6-month disease-free interval, and 10-month overall survival. Our results demonstrate that the poor clinical outcome of this patient was associated with pronounced, more than fivefold higher, overexpression of both cFOS and TGF-beta 1 proteins in its metastatic nodal tissue extracts, when compared with the values of the two non-TNBC controls (with zero disease-free interval and overall survival). This original observation suggests, for the first time, that both the cFOS and TGF-beta 1 may be considered as a pair of biomarkers for an early assessment of poor prognosis for TNBC patients. The possible clinical implication of this observation is discussed

Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source

OBJECTIVE: To use cytoplasmic tissue extract as a new specimen source to quantify transforming growth factor beta 1 (TGF beta 1) protein in metastatic axillary lymph node tissue (ALNT) of breast cancer (BC) patients and to confirm the feasibility of this approach in a prospective pilot study on a subgroup of patients with invasive BC. STUDY DESIGN: The 6 selected malignant and autologous nonmalignant pairs of ALNT were fractionated, under special preanalytical, nonaggressive/nondenaturing conditions, to obtain respective cytoplasmic extracts for TGF beta 1 detection by the Quantikine (R and D Systems Inc., Minneapolis, Minnesota, U.S.A.) enzyme-linked immunosorbent assay kit. RESULTS: The data indicated a highly significant (r=0.973054) positive linear correlation between the TGF beta 1 concentration and total protein concentration in cytoplasmic extract of metastatic ALNT. The subsequent patients pilot study, performed strictly before any clinicopathologic factors were accessible, revealed significantly (p LT 0.01) elevated TGF beta 1 in malignant ALNT (median value: 1.05 ng/mg protein, range: 0.67-3.6 ng/mg protein, n=6) vs. autologous nonmalignant ALNT controls (median value: 0.48 ng/mg protein, range: 0.29-0.90 ng/mg protein, n=6). This elevation was correlated with the number of metastatic axillary lymph nodes with respect to the total and was consistent with an increase in size of tumor deposits in axillary lymph nodes. CONCLUSION: Our data provide for the first time suggestive evidence that the TGF beta 1 level in cytoplasmic extracts of metastatic ALNTs may be a promising bio-marker of invasiveness for BC patients. Confirmatory, large-scale studies are needed to evaluate possible implications of this putative biomarker in BC diagnosis and treatment. (Anal Quant Cytol Histol 2009;31:288-295

Elevated plasma TGF-beta(1) levels correlate with decreased survival of metastatic breast cancer patients

Background: The role of circulating TGF-beta(1) in prognosis of breast cancer (BC) was investigated with an intention to define TGF-beta(1)-dependent high risk and low risk subsets of patients. Methods: Fifty three BC patients of all clinical stages and 37 healthy donors (HD) were analyzed for plasma TGF-beta(1) by the T beta RII receptor-based Quantikine TGF-beta(1) ELISA kit. Results: The plasma TGF-beta(1) level of Stage I/II disease (median: 0.94 ng/ml; n=10)) remained close to HD (median: 1.30 ng/ml; n = 37; p GT 0.1). In contrast, Stage III/IV disease (median: 2.34 ng/ml; n=43) exhibited highly significant TGF-beta(1) elevation (p LT 0.001) relative to HD. Further analysis revealed that TGF-beta(1) increase was predominantly attributed to Stage IV, metastatic disease patients (Q3=4.23 ng/ml) rather than to the group Stage III/IV (Q3=3.58 ng/ml). Using the plasma TGF-beta(1) concentration of 3.00 ng/ml as the cut-off value, two subgroups of patients were formed. Overall 2-year survival of the first subgroup, having elevated plasma TGF-beta(1) ( GT 3.00 ng/ml; n=10), was 10%. This was significantly decreased (p LT 0.05) compared to 52% survival observed for the second subgroup of patients with plasma TGF beta(1) values close to HD ( LT 3.00 ng/ml, n=19). Conclusion: We have performed a pilot study to determine the relationship between overall survival and TGF-beta(1) concentration in the blood of metastatic breast cancer patients. The survival was significantly reduced in the patients with elevated plasma TGF-beta(1) levels compared to that of the patients with plasma TGF-beta(1) levels close to normal. We propose that plasma TGF-beta(1) concentration may be a new tumour marker attributed to the presence of metastatic BC cells that may be used in selection of metastatic BC patients with poor prognosis. (c) 2006 Elsevier B.V. All rights reserved

Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome

We report on characteristics of the first human cell line, PC-MDS, derived from a bone marrow of a patient with therapy-related myelodysplastic syndrome (t-MDS) who had no overt post-MDS leukemia. Classic cytology analyses, immunophenotyping, cytogenetic and molecular genetic procedures were used for characterization of the cell line. PC-MDS cells are positive for the expression of CD13, CD15, CD30, CD33, and CD45 antigen. Positive cytochemical staining and immunophenotype analyses indicated that PC-MDS cells have some characteristics of the early myeloid precursor cell. The karyotype analysis of PC-MDS cell line revealed various numerical and structural changes including those typically associated with t-MDS: del(5)(q13)[7], der(5)t(5;11)(p11;q11)[13], -7[6], del(7)(q31)[2], +20[3], -20[4]. Evaluation of methylation status in a promoter region of p 15, p 16 and MGMT genes showed biallelic hypermethylation pattern of 5 promoter region only in MGMT gene. PC-MDS is the first t-MDS derived cell line, and based on its immunological, cytogenetic and molecular characterization could be a new tool in evaluation of complex biology of MDS and a model for methylation studies. (c) 2007 Elsevier Ltd. All rights reserved