350 research outputs found
Spectrum of antibacterial activity and mode of action of a novel tris-stilbene bacteriostatic compound
The spectrum of activity and mode of action of a novel antibacterial agent, 135C, was investigated using a range of microbiological and genomic approaches. Compound 135C was active against Gram-positive bacteria with MICs for Staphylococcus aureus ranging from 0.12–0.5 μg/ml. It was largely inactive against Gram-negative bacteria. The compound showed bacteriostatic activity in time-kill studies and did not elicit bacterial cell leakage or cell lysis. Checkerboard assays showed no synergy or antagonism when 135C was combined with a range of other antibacterials. Multi-step serial passage of four S. aureus isolates with increasing concentrations of 135C showed that resistance developed rapidly and was stable after drug-free passages. Minor differences in the fitness of 135C-resistant strains and parent wildtypes were evident by growth curves, but 135C-resistant strains did not show cross-resistance to other antibacterial agents. Genomic comparison of resistant and wildtype parent strains showed changes in genes encoding cell wall teichoic acids. 135C shows promising activity against Gram-positive bacteria but is currently limited by the rapid resistance development. Further studies are required to investigate the effects on cell wall teichoic acids and to determine whether the issue of resistance development can be overcome
Assembly of the Inner Perivitelline Layer, a Homo log of the Mammalian Zona Pellucida: An Immunohistochemical and Ultrastructural Study
The avian inner perivitelline layer (IPVL), a homologous structure to the mammalian zona pellucida, is deposited between the granulosa cells and the oocyte cell membrane during folliculogenesis. The glycoprotein meshwork of the IPVL forms a 3-dimensional matrix and possesses important functions in the fertilization process: it contributes to the binding of avian spermatozoa to the oocyte and induces acrosomal exocytosis. In contrast to the zona pellucida of mammals, the IPVL does not prevent the physiological polyspermy found in birds. Previous studies have shown that in the Japanese quail (Cotumix japonica) at least 5 glycoproteins are constituents of the IPVL (ZP1, ZP2, ZP3, ZP4, and ZPD). In this study, we investigated the spatiotennporal assembly pattern of the IPVL during folliculogenesis using immunohistochemical and ultrastructural methods. The obtained results clearly show that these glycoproteins are incorporated into the IPVL at distinct points during follicular development, supporting the hypothesis that ZP2 and ZP4 form a type of prematrix into which ZP1, ZP3, and ZPD are integrated at a later stage of development. Copyright (C) 2011 S. Karger AG, Base
Debris disk size distributions: steady state collisional evolution with P-R drag and other loss processes
We present a new scheme for determining the shape of the size distribution,
and its evolution, for collisional cascades of planetesimals undergoing
destructive collisions and loss processes like Poynting-Robertson drag. The
scheme treats the steady state portion of the cascade by equating mass loss and
gain in each size bin; the smallest particles are expected to reach steady
state on their collision timescale, while larger particles retain their
primordial distribution. For collision-dominated disks, steady state means that
mass loss rates in logarithmic size bins are independent of size. This
prescription reproduces the expected two phase size distribution, with ripples
above the blow-out size, and above the transition to gravity-dominated
planetesimal strength. The scheme also reproduces the expected evolution of
disk mass, and of dust mass, but is computationally much faster than evolving
distributions forward in time. For low-mass disks, P-R drag causes a turnover
at small sizes to a size distribution that is set by the redistribution
function (the mass distribution of fragments produced in collisions). Thus
information about the redistribution function may be recovered by measuring the
size distribution of particles undergoing loss by P-R drag, such as that traced
by particles accreted onto Earth. Although cross-sectional area drops with
1/age^2 in the PR-dominated regime, dust mass falls as 1/age^2.8, underlining
the importance of understanding which particle sizes contribute to an
observation when considering how disk detectability evolves. Other loss
processes are readily incorporated; we also discuss generalised power law loss
rates, dynamical depletion, realistic radiation forces and stellar wind drag.Comment: Accepted for publication by Celestial Mechanics and Dynamical
Astronomy (special issue on EXOPLANETS
Scenario of Accelerating Universe from the Phenomenological \Lambda- Models
Dark matter, the major component of the matter content of the Universe,
played a significant role at early stages during structure formation. But at
present the Universe is dark energy dominated as well as accelerating. Here,
the presence of dark energy has been established by including a time-dependent
term in the Einstein's field equations. This model is compatible with
the idea of an accelerating Universe so far as the value of the deceleration
parameter is concerned. Possibility of a change in sign of the deceleration
parameter is also discussed. The impact of considering the speed of light as
variable in the field equations has also been investigated by using a well
known time-dependent model.Comment: Latex, 9 pages, Major change
Reply to editorial and commentaries on Steele, Al-Mufti, Augustyn, Chandrajith, Coghlan, Coulson et al. (2018) "Cause of Cambrian explosion - Terrestrial or cosmic?"
No abstract availabl
Reply to commentary by R Duggleby (2019)
Duggleby (2018) has made a numerical analysis of some aspects of the wide range of phenomena we reviewed in Steele et al. (2018) and asserted " .that panspermia as proposed by Steele et al. (2018) is extremely implausible.” It seems to us that Duggleby has based his viewpoint on a quite narrow and specific model of Panspermia which he supposes to be active in the cosmos. Here we address both his conclusions and his numerical analysis. Our response therefore will be at two levels, his specific analysis and his general conclusions. In the specific section below we show that while Duggleby's numerical analysis appears in part correct it is, in the final analysis, quite irrelevant to Cosmic Panspermia. In the general response which follows we address his unsupported conclusion throughout his critique, namely that … " none of the examples mentioned by Steele et al. (2018) is decisive enough to allow no other explanation.
Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
Modelling thirty-day mortality in the acute respiratory distress syndrome (ARDS) in an adult ICU
Publisher's copy made available with the permission of the publisher © Australian Society of AnaesthetistsVariables predicting thirty-day outcome from Acute Respiratory Distress Syndrome (ARDS) were analysed using Cox regression structured for time-varying covariates. Over a three-year period, 1996-1998, consecutive patients with ARDS (bilateral chest X-ray opacities, PaO₂/FiO₂ ratio of <200 and an acute precipitating event) were identified using a prospective computerized data base in a university teaching hospital ICU. The cohort, 106 mechanically ventilated patients, was of mean (SD) age 63.5 (15.5) years and 37% were female. Primary lung injury occurred in 45% and 24% were postoperative. ICU-admission day APACHE II score was 25 (8); ARDS onset time from ICU admission was 1 day (median: range 0-16) and 30 day mortality was 41% (95% CI: 33%-51%). At ARDS onset, PaO₂/FiO₂ ratio was 92 (31), 81% had four-quadrant chest X-ray opacification and lung injury score was 2.75 (0.45). Average mechanical ventilator tidal volume was 10.3 ml/ predicted kg weight. Cox model mortality predictors (hazard ratio, 95% CI) were: APACHE II score, 1.15 (1.09-1.21); ARDS lag time (days), 0.72 (0.58-0.89); direct versus indirect injury, 2.89 (1.45-5.76); PaO₂/FiO₂ ratio, 0.98 (0.97-0.99); operative versus non-operative category, 0.24 (0.09-0.63). Time-varying effects were evident for PaO₂/FiO₂ ratio, operative versus non-operative category and ventilator tidal volume assessed as a categorical predictor with a cut-point of 8 ml/kg predicted weight (mean tidal volumes, 7.1 (1.9) vs 10.7 (1.6) ml/kg predicted weight). Thirty-day survival was improved for patients ventilated with lower tidal volumes. Survival predictors in ARDS were multifactorial and related to patient-injury-time interaction and level of mechanical ventilator tidal volume.J. L. Moran, P. J. Solomon, V. Fox, M. Salagaras, P. J. Williams, K. Quinlan, A. D. Berstenhttp://www.aaic.net.au/Article.asp?D=200332
From Clock Synchronization to Dark Matter as a Relativistic Inertial Effect
Lecture at BOSS2011 on relativistic metrology, on clock synchronization,
relativistic dynamics and non-inertial frames in Minkowski spacetime, on
relativistic atomic physics, on ADM canonical tetrad gravity in asymptotically
Minkowskian spacetimes, on the York canonical basis identifying the inertial
(gauge) and tidal degrees of freedom of the gravitational field, on the
Post-Minkowskian linearization in 3-orthogonal gauges, on the Post-Newtonian
limit of matter Hamilton equations, on the possibility to interpret dark matter
as a relativistic inertial effect connected with relativistic metrology (i.e.
clock synchronization) in Einstein GR.Comment: 90 pages. Lecture at BOSS201
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